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Biochemical and biophysical research communications v.494 no.3/4, 2017년, pp.621 - 625   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Crystal structures of Aflatoxin-oxidase from Armillariella tabescens reveal a dual activity enzyme

Xu, Tingting (School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China ); Xie, Chunfang (National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, 510632, China ); Yao, Dongsheng (National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, 510632, China ); Zhou, Cong-Zhao (School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China ); Liu, Jinsong (State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China );
  • 초록  

    Abstract Aflatoxin-oxidase (AFO), a newly discovered oxidase isolated from Armillariella tabescens , was reported to perform aflatoxin B1 (AFB1) detoxification through breaking the bisfuran ring of AFB1. However, based on sequence alignment, we found that AFO shares high sequence identities with dipeptidyl peptidase III (DPP III) family members. To understand the functions of AFO, we determined its crystal structures in the absence and presence of zinc, copper ion, and employed HPLC to test if AFO could cleave the substrates of DPP III. Our structures reveal that AFO contains the classic DPP III activity center and the HPLC results further confirm that AFO possesses the dipeptidyl peptidase activity. Therefore, AFO should belong to DPP III family. Interestingly, unlike reported classic DPP III structure that has a large domain movement upon substrate binding, the AFO structures all adopt the closed conformation, independent of substrate binding. This conformation characteristic of AFO may be related to its enzyme activities. Taken together, our results demonstrate that AFO is a dual activity enzyme with both aflatoxin-oxidase and dipeptidyl peptidase activities and its unique conformation feature expands our understanding on the mode of reaction for this enzyme family. Highlights We determined the crystal structure of Aflatoxin-oxidase (AFO). AFO belongs to the DPP III family and possesses dipeptidyl peptidase activity. AFO has a unique conformation characteristic. AFO is a dual activity enzyme.


  • 주제어

    Crystal structure .   Aflatoxin-oxidase .   Dipeptidyl peptidase III.  

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