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Biochemical and biophysical research communications v.494 no.3/4, 2017년, pp.634 - 640   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Investigation on temperature-induce conformational change of immobilized β 2 adrenergic receptor

Gao, Xiaokang (School of Pharmaceutical Sciences, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei Provincial Technology and Research Center for Comprehensive Development of Medicinal Herbs, Hubei University of Medicine, Shiyan 442000, Hubei, China ) ; Yang, Lingjian (College of Life Science, Northwest University, Xi'an 710069, China ) ; Li, Qian (College of Life Science, Northwest University, Xi'an 710069, China ) ; An, Yuxin (College of Life Science, Northwest University, Xi'an 710069, China ) ; Liao, Sha (College of Life Science, Northwest University, Xi'an 710069, China ) ; Gao, Haiyang (College of Life Science, Northwest University, Xi'an 710069, China ) ; Zhao, Xinfeng (College of Life Science, Northwest University, Xi'an 710069, China ) ; Bian, Liujiao (College of Life Science, Northwest University, Xi'an 710069, China ) ; Zheng, Xiaohui (College of Life Science, Northwest University, Xi'an 710069, China ) ;
  • 초록  

    Abstract The β 2 adrenergic receptor ( β 2 -AR) is a prototypical family A G protein-coupled receptor (GPCR) and an excellent model system for studying the mechanism of GPCR activation. Purified β 2 -AR was immobilized on macroporous silica gel to obtain liquid chromatographic stationary phase. The resulting phase was packed into a stainless steel column (4.6 × 50 mm, 7 μm) and used for on-line chromatographic system. When column oven temperature increased from 20.0 °C to 40.0 °C, uncomplete separate chromatographic peaks of ephedrine and pseudoephedrine as receptor conformational probe were gradually merged into one peak, meanwhile retention time and resolution of the probes were reduced correspondingly, which suggested that temperature could regulate protein conformation. Temperature-induced conformational change of immobilized β 2 -AR, especially changes at higher temperatures, indicated that constructed receptor chromatography could simulate fever disease state of human body and clarify receptor conformation change at pathological condition. At the same time this study could also provide new ideas for screening active components in pathological conditions. Highlights Temperature could regulate protein conformational. The receptor chromatography can simulate the pathological conditions of human fever.


  • 주제어

    β 2 adrenergic receptor .   Temperature-induced .   Protein conformation .   Receptor chromatography .   Ephedrine and pseudoephedrine.  

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