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Biochemical and biophysical research communications v.494 no.3/4, 2017년, pp.663 - 667   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

PYK2 mediates BzATP-induced extracellular matrix proteins synthesis

Torigoe, Go (Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan ) ; Nagao, Mayu (Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan ) ; Tanabe, Natsuko (Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan ) ; Manaka, Soichiro (Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan ) ; Kariya, Taro (Department of Orthodontics, Nihon University School of Dentistry, Tokyo, Japan ) ; Kawato, Takayuki (Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan ) ; Sekino, Jumpei (Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan ) ; Kato, Shunichiro (Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan ) ; Maeno, Masao (Nihon University, Tokyo, Japan ) ; Suzuki, Naoto (Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan ) ; Shimizu, Noriyoshi (Department of Orthodontics, Nihon University School of Dentistry, Tokyo, Japan ) ;
  • 초록  

    Abstract Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli. Furthermore, activation of PYK2 also was a response to integrin by mechanical stimuli. Extracellular matrix protein (ECMP)s, which are important factors for bone formation are expressed by osteoblasts. However, the effect of the interaction of 2’(3)- Ο -(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP), which is the agonist of the mechanosensitive receptor P2X7, with PYK2 on ECMP production is poorly understood. Thus, our purpose was to investigate the effects of PYK2 on BzATP-induced ECMP production in osteoblasts. BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression. These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation.


  • 주제어

    PYK2 .   BzATP .   P2X7 .   Mechanical stimuli .   Extracellular matrix proteins.  

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