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Ecotoxicology and environmental safety v.147, 2018년, pp.266 - 274   SCI SCIE
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Soil properties influence kinetics of soil acid phosphatase in response to arsenic toxicity

Wang, Ziquan (College of Natural Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Yangling, 712100 Shaanxi, China ) ; Tan, Xiangping (College of Natural Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Yangling, 712100 Shaanxi, China ) ; Lu, Guannan (College of Natural Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Yangling, 712100 Shaanxi, China ) ; Liu, Yanju (Global Centre for Environmental Research, The Faculty of Science and Information Technology, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia ) ; Naidu, Ravi (Global Centre for Environmental Research, The Faculty of Science and Information Technology, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia ) ; He, Wenxiang (College of Natural Resources and Environment ) ;
  • 초록  

    Abstract Soil phosphatase, which plays an important role in phosphorus cycling, is strongly inhibited by Arsenic (As). However, the inhibition mechanism in kinetics is not adequately investigated. In this study, we investigated the kinetic characteristics of soil acid phosphatase (ACP) in 14 soils with varied properties, and also explored how kinetic properties of soil ACP changed with different spiked As concentrations. The results showed that the Michaelis constant ( K m ) and maximum reaction velocity ( V max ) values of soil ACP ranged from 1.18 to 3.77mM and 0.025–0.133mMh −1 in uncontaminated soils. The kinetic parameters of soil ACP in different soils changed differently with As contamination. The K m remained unchanged and V max decreased with increase of As concentration in most acid and neutral soils, indicating a noncompetitive inhibition mechanism. However, in alkaline soils, the K m increased linearly and V max decreased with increase of As concentration, indicating a mixed inhibition mechanism that include competitive and noncompetitive. The competitive inhibition constant ( K ic ) and noncompetitive inhibition constant ( K iu ) varied among soils and ranged from 0.38 to 3.65mM and 0.84–7.43mM respectively. The inhibitory effect of As on soil ACP was mostly affected by soil organic matter and cation exchange capacity. Those factors influenced the combination of As with enzyme, which resulted in a difference of As toxicity to soil ACP. Catalytic efficiency ( V max / K m ) of soil ACP was a sensitive kinetic parameter to assess the ecological risks of soil As contamination. Highlights pH and amorphous Fe explained 50.8–66.1% and 29.8–43.8% of soil ACP V max , K m and V max / K m variations across soils; The type of As inhibition on soil ACP was noncompetitive or mixed inhibition mainly depend on soil types; Soil organic matter and cation exchange capacity were major controlling factors affecting As inhibitory effect on soil ACP. Catalytic efficiency ( V max / K m ) could sensitively indicate the toxicity of As to soil ACP.


  • 주제어

    Arsenic .   Soil acid phosphatase .   Soil property .   Inhibition constant .   Noncompetitive inhibition.  

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