Lethal and sublethal effects of the pirimicarb-based formulation Aficida® on Boana pulchella (DumEril and Bibron, 1841) tadpoles (Anura, Hylidae)
Abstract Acute lethal and sublethal toxicity of the pirimicarb-based commercial formulation Aficida ? were evaluated on Boana pulchella tadpoles. Whereas mortality was used as end point for lethality, frequency of micronuclei and other nuclear abnormalities as well as alterations in the frequency of erythroblasts in circulating blood as biomarkers for genotoxicity and cytotoxicity, respectively. Swimming, growth, developmental and morphological abnormalities were also employed as sublethal end points. Results show that the species is within the 13th percentile of the distribution of acute sensitivity of species to pirimicarb for aquatic vertebrates. Results revealed values of 23.78 and 101.45mg/L pirimicarb as LC50 96h for GS25 and GS36 tadpoles, respectively. The most evident effects were related with the swimming activity with NOEC and LOEC values within the 0.005–0.39mg/L pirimicarb concentration range. Aficida ? induced DNA damage at the chromosomal level by increasing micronuclei frequency and other nuclear abnormalities, i.e. , lobbed and notched nuclei and binucleated cells. Cellular cytotoxicity was found after Aficida ? treatment. The presence of abdominal oedemas in exposed organisms and thus flotation response of organisms could be proposed as a new sensitive exposure parameter. The multiple end point assessment approach used allowed a complete understanding the multi level of effects occurring by exposure to pirimicarb, at least in B. pulchella . Highlights Pirimicarb-based formulation Aficida ? jeopardizes anuran tadpoles. Acute lethal and sublethal toxicity of Aficida ? were evaluated on Boana pulchella . The LC50 96h for GS25 and GS36 tadpoles were 60.95 and 260.02mg/L Aficida ? , respectively. Flotation of organisms is proposed as a new sensitive biomarker. Aficida ? induced DNA damage at the chromosomal level.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기