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Ecotoxicology and environmental safety v.147, 2018년, pp.1056 - 1064   SCI SCIE
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Genes coding for transporters showed a rapid and sharp increase in their expression in response to lead, in the aquatic fern (Salvinia minima Baker)

Leal-Alvarado, D.A. (Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná ) ; Estrella-Maldonado, H. (de Hidalgo, 97200 Mérida, Yucatán, Mexico ) ; Sáenz-Carbonell, L. (Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná ) ; Ramírez-Prado, J.H. (de Hidalgo, 97200 Mérida, Yucatán, Mexico ) ; Zapata-Pérez, Omar (Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná ) ; Santamaría, J.M. (de Hidalgo, 97200 Mérida, Yucatán, Mexico ) ;
  • 초록  

    Abstract Salvinia minima was assessed for its ability to accumulate lead (Pb) by exposing it to concentrations of 40μM Pb(NO 3 ) 2 during 24h. At the same time, the expression levels were quantified, of four genes coding for transporters: SmABCC (ABCC-MRP), SmATPase (ATPase-P3A), SmNhaD (Type-Na + /H + ) and SmABCG (ABCG-WBC). In the absence of lead, S. minima had very low expression of those genes, when plants were exposed to the metal however, those genes showed a rapid (in just three hours or less) and sharp increase (up to 60 times) in their expression, particularly the SmNhaD (Type-Na + /H + ) gene. This sharp increase in expression levels of the genes studied, occurred at the same time that the plant accumulated the highest content of lead in its tissues. The first two genes, are apparently implicated in detoxification and lead accumulation mechanisms, while the other two genes are apparently involved in maintaining cell balance (homeostatic control) and membrane integrity. Our results confirmed that S. minima is efficient for phytoremediation of water bodies contaminated by lead, as it is efficient in accumulating this metal in its tissues (bioconcentration factor; BCF) values greater than 1000, in short times of exposure. More importantly, our data on the expression profiles of four genes coding for transporters, represent a first sight scenario of the molecular basis for understanding the different mechanism of detoxification, apparently present in this aquatic fern. Highlights We confirmed the high capacity of S. minima Baker to uptake and accumulate Pb, in its tissues. We characterize four genes that codify for transporters in response to Pb exposure in S. minima Baker. Those genes were classified using Gene Onthology and phylogenetic tree analysis. Gene expression levels were quantified by qRT-PCR at different times during a 48h exposure to 40uM of Pb(NO 3 ) 2 . Our results contribute to the understanding of the molecular basis of the high capacity of this fern, to uptake and tolerate Pb.


  • 주제어

    Lead uptake .   Accumulation .   Bioconcentration factor (BCF) .   Gene expression .   Salvinia minima.  

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