본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Molecular and cellular neurosciences v.85, 2017년, pp.19 - 28   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

The expression of pluripotency and neuronal differentiation markers under the influence of electromagnetic field and nitric oxide

Haghighat, Nazanin (Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran ) ; Abdolmaleki, Parviz (Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran ) ; Parnian, Javad (Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran ) ; Behmanesh, Mehrdad (Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran ) ;
  • 초록  

    Abstract Nitric oxide (NO) is a diatomic free radical compound that as a secondary messenger contributes to cell physiological functions and its variations influence proteins activity and triggering intracellular signaling cascades. Low frequency electromagnetic field (EMF) alters the cell biology such as cell differentiation by targeting the plasma membrane and entering force to the ions and small electrical ligands. The effect of these chemical (NO) and physical (EMF) factors on the expression of the stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells (BMSC) was investigated. The cells were treated with low (50micromolar) and high (1mM) concentrations of Deta-NO as a NO donor molecule and 50Hz low frequency EMF. The expression of pluripotency and neuronal differentiation genes and proteins was investigated using real time qPCR and Immunocytochemistry techniques. The simultaneous treatment of EMF with NO (1mM) led to the down-regulation of stemness markers expression and up-regulation of neuronal differentiation markers expression. Cell proliferation decreased and cell morphology changed which caused the majority of cells obtains neuronal protein markers in their cytoplasm. The decrease in the expression of neuronal differentiation Nestin and DCX markers without any change in the expression of pluripotency Oct4 marker (treated with low concentration of NO) indicates protection of stemness state in these cells. Treatment with NO demonstrated a double behavior. NO low concentration helped the cells protect the stemness state but NO high concentration plus EMF pushed cells into differentiation pathway. Highlights BMSCs have different behaviors in low and high concentrations of NO. Low concentration of NO helped to protect of pluripotency state of BMSCs. High concentration of NO plus electromagnetic field led to express the neuronal differentiation markers in more cells. EMF helped polarization of cells so more cells achieved morphology like neurons. Differentiation of BMSCs into neuronal cells was facilitated by simultaneous treatment of EMF and NO (1mM).


  • 주제어

    DCX .   Differentiation .   Electromagnetic field .   Immunocytochemistry .   Nanog .   Nestin .   Nitric oxide .   Oct4 .   Proliferation .   Retinoic acid .   Real time qPCR.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기