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Microbial pathogenesis v.112, 2017년, pp.76 - 82   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

In vivo safety evaluation of antibacterial silver chloride nanoparticles from Streptomyces exfoliatus ICN25 in zebrafish embryos

Iniyan, Appadurai Muthamil (International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari Dist 629502, TN, India ) ; Kannan, Rajaretinam Rajesh (Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, TN, India ) ; Joseph, Francis-Joseph Rosemary Sharmila (International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari Dist 629502, TN, India ) ; Mary, Thankaraj Rajam Jabila (Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, TN, India ) ; Rajasekar, Mani (Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, TN, India ) ; Sumy, Puthenpurayil Chellappan (International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technol ) ; Rabel, Arul Maximus ; Ramachandran, Dasnamoorthy ; Vincent, Samuel Gnana Prakash ;
  • 초록  

    Abstract Silver chloride nanoparticles were synthesized from the cell-free culture supernatant of Streptomyces strain using green synthesis approach with good yield. The nanoparticles were characterized by UV-Vis, IR, SEM, AFM and XRD techniques. These nanoparticles exhibited broad spectrum of antibacterial activity towards Methicillin-resistant Staphylococcus aureus , Methicillin sensitive S. aureus , Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia at ≤ 2 μg/ml minimal inhibitory concentrations. In vivo bioassay in nanoparticles treated zebrafish embryos exhibited 16 μg/ml dose as maximal cardiac safety concentration and further increases in concentration revealed adverse effects such as pericardial bulging, mouth protrudation, hemorrhage and yolk sac elongation. The less toxicity of nanoparticles treated embryos in terms of cardiac assessment and lethality analysis was observed. The dose below 5 μg/ml is concluded as an in vitro and in vivo therapeutic dose. The properties of this biosynthesized nanoparticle suggest a path towards developing antibiotic nanoparticles that are likely to avoid development of multidrug resistance. Highlights Antibacterial silver chloride nanoparticles were synthesized from Streptomyces strain using green synthesis approach. In vivo bioassay in zebrafish embryos were studied and cardiac safety concentration was reported. The biosynthesized nanoparticles showed potent cytotoxicity towards HeLa and SiHa Cancer cell lines.


  • 주제어

    Nano-biotechnology .   Silver chloride nanoparticle .   Streptomyces .   Zebrafish .   Cardiac assessment .   MTT assay.  

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