본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Microbial pathogenesis v.112, 2017년, pp.164 - 170   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Analysis of resistance genes in pan-resistant Myroides odoratimimus clinical strain PR63039 using whole genome sequencing

Ming, De-song (Department of Clinical Diagnostics, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian 362000, China ) ; Chen, Qing-qing (Department of Clinical Diagnostics, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian 362000, China ) ; Chen, Xiao-tin (Department of Clinical Diagnostics, Quanzhou First Hospital affiliated to Fujian Medical University, Quanzhou, Fujian 362000, China ) ;
  • 초록  

    Abstract To clarify the antibiotic resistance mechanisms of Myroides odoratimimus , pan-resistant M. odoratimimus strain PR63039 was isolated and its genome sequenced and analyzed. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method, and the Phoenix-100 Automated Microbiology System with a NMIC/ID-4 panel including aminoglycosides, β-lactams, polypeptides, quinolones, sulfonamides, chloramphenicols, and tetracyclines. Single-molecule real-time whole genome sequencing was conducted using the PacBio RSII system, and genome annotation was performed using RAST and IMG ER. To characterize the genome features, a number of databases and software programs, including GC-Profile, CG viewer, the VFDB database, ISfinder, RADB, CARD, ResFinder, and PHAST, were used. M. odoratimimus isolate PR63039 was resistant to almost all antibiotics tested, suggesting pan-drug resistance. The genome consisted of a 4,366,950-bp chromosome and a 90,798-bp plasmid (p63039), which contained a large number of resistance genes and virulence factors. The distribution of the resistance genes was distinctive, and a resistance region, designated MY63039-RR, was identified. RAST analysis indicated that 108 of the annotated genes were potentially involved in virulence, disease, and defense, all of which could be associated with resistance and pathogenicity. Prophage analysis also identified two incomplete prophages in the genome of M. odoratimimus PR63039. Multiple antibiotic-resistance genes were identified, including those associated with resistance to tetracycline ( tetX ), macrolides ( ereB , cfrA , lasE ), sulfonamides ( sul2, sul3 ), β-lactams ( bla MUS-1 , bla TUS-1 , bla SFB-1 , bla SLB-1 , bla OXA-209 , bla OXA-347 ), and chloramphenicol ( cat ). Further, the presence of 18 antibiotic efflux pump-encoding resistance genes, including acrB , acrD , acrF , adeB , adeG , adeJ , amrB , ceoB , cmeB , mdsB , mexB , mexD , mexF , mtrD , smeE , mdtF , macB , likely accounts for the observed quinolone resistance of strain PR63039. To the best of our knowledge, this is the first report of the presence of the bla SFB-1 , bla SLB-1 , bla OXA-209 , bla OXA-347 , and tetX resistance genes in M. odoratimimus. Highlights We determined the complete genome sequence of a clinical pan-resistant M. odoratimimus strain PR63039. The M.odoratimimus genome consists of chromosome (GenBank no.CP013690 and no. CP013691) and a plasmid p630. The M.odoratimimus genome contains many resistance genes, including beta-lactamase, tetX , EreB , cfrA, lasE , sul2 , sul3 , cat. This is the first report of presence of the SFB-1, SLB-1, OXA-209, OXA-347, tetX resistance genes in M. odoratimimus.


  • 주제어

    Myroides odoratimimus .   Whole genome sequencing .   Resistance .   Quinolones.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기