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Microbial pathogenesis v.112, 2017년, pp.209 - 214   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Evaluation of immunological responses to recombinant Porin A protein (rPoA) from native strains of Neisseria meningitidis serogroups A and B using OMV as an adjuvant in BALB/c mice

Afrough, Parviz (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ; Bouzari, Saeid (Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran ) ; Mousavi, Seyed Fazlollah (Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran ) ; Asadi Karam, Mohammad Reza (Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran ) ; Vaziri, Farzam (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ; Fateh, Abolfazl (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ; Behrouzi, Ava (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ; Malekan, Mohammadali (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ; Siadat, Seyed Davar (Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran ) ;
  • 초록  

    Abstract Neisseria meningitidis is one of the main causes of sepsis and meningitis, which are two serious life-threatening diseases in both children and adolescents. Porin A ( porA ) from both serogroup A and B were cloned into the pET28a plasmid and expressed in E. coli BL21 (DE3). The protein was expressed in Escherichia coli BL21 (DE3) and confirmed by SDS-PAGE and Western blot analysis. BALB/c mice were subcutaneously injected three times with 25 μg of the recombinant PorA. Specific total IgG antibodies and isotypes were evaluated using ELISA assay. Opsonophagocytic assay (OPA) and Serum Bactericidal assay (SBA) were performed. Results showed that vaccinated mice exhibited higher levels of anti-Porin A (p N. meningitidis was opsonized with immunized-mice sera, and compared to non-immunized mice, immunized mice displayed significantly increased phagocytic uptake and effective intracellular killing. In this study, serogroup B N. meningitidis OMV of strain CSBPI G-245 and complete and incomplete Freund's adjuvant were used. Results demonstrated that Porin A could be a valuable target for the development of immunotherapeutic strategies against N. meningitidis . Highlights This study designed new construct as a candidate vaccine against Neisseria meningitides . Our results were shown high significance of IgG, IgG1 and especially IgG2a. Finally, protective properties against infections confirmed by opsonophagocytosis assay and bactericidal assay. Our results showed Por (A + B) mixed with OMV as an adjuvant may be mentioned as the candidate vaccine against infections caused by Neisseria meningitidis.


  • 주제어

    Meningococcal .   PorA .   OMV .   Vaccine .   Immunological responses.  

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