The pathogenicity and biological features of Santee-Cooper Ranaviruses isolated from Chinese perch and snakehead fish
Abstract Ranavirus has become a noticeable threat to both farmed and natural populations of fish and amphibians. Herein, we reported that 3 strains of novel viruses, designated as ScRIV-GM-20150902, CmRIV-XT-20150917 and ScRIV-ZS-20151201, were isolated from diseased Chinese perch and snakehead fish in China. Efficient propagation of these isolates were determined in Chinese perch brain (CPB) cell line by the means of cytopathic effect observation, PCR amplification and electron microscopy observation. And their viral titers in CPB cells reached 10 8.13 TCID 50 ml −1 , 10 7.71 TCID 50 ml −1 and 10 7.94 TCID 50 ml −1 , respectively. While the challenge experiment results showed that 3 isolates resulted in 100% mortality of Chinese perch after virus infection. Electron microscopy analysis showed that two kinds of viral inclusion bodies (intracytoplasmic and intranuclear inclusion body) were observed in infected CPB cells. Sequence alignment and phylogenetic analysis of major capsid protein gene sequences of isolates revealed that these isolates belonged to the species Santee-Cooper Ranavirus. Highlights Three novel ranaviruses were isolated and viral titers were determined in CPB cell lines. These isolates are belonging to Santee-Cooper ranaviruses by MCP genes sequences analysis. Two kinds of virus inclusion bodies were observed in infected CPB cells.
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