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Metabolic engineering v.44, 2017년, pp.108 - 116   SCIE
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Tat proteins as novel thylakoid membrane anchors organize a biosynthetic pathway in chloroplasts and increase product yield 5-fold

Henriques de Jesus, Maria Perestrello Ramos (Copenhagen Plant Science Center, Center for Synthetic Biology “bioSYNergy”, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark ) ; Zygadlo Nielsen, Agnieszka (Copenhagen Plant Science Center, Center for Synthetic Biology “bioSYNergy”, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark ) ; Busck Mellor, Silas (Copenhagen Plant Science Center, Center for Synthetic Biology “bioSYNergy”, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark ) ; Matthes, Annemarie (Copenhagen Plant Science Center, Center for Synthetic Biology “bioSYNergy”, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark ) ; Burow, Meike (DynaMo Center, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark ) ; Robinson, Colin (School of Biosciences, ) ; Erik Jensen, Poul ;
  • 초록  

    Abstract Photosynthesis drives the production of ATP and NADPH, and acts as a source of carbon for primary metabolism. NADPH is also used in the production of many natural bioactive compounds. These are usually synthesized in low quantities and are often difficult to produce by chemical synthesis due to their complex structures. Some of the crucial enzymes catalyzing their biosynthesis are the cytochromes P450 (P450s) situated in the endoplasmic reticulum (ER), powered by electron transfers from NADPH. Dhurrin is a cyanogenic glucoside and its biosynthesis involves a dynamic metabolon formed by two P450s, a UDP-glucosyltransferase (UGT) and a P450 oxidoreductase (POR). Its biosynthetic pathway has been relocated to the chloroplast where ferredoxin, reduced through the photosynthetic electron transport chain, serves as an efficient electron donor to the P450s, bypassing the involvement of POR. Nevertheless, translocation of the pathway from the ER to the chloroplast creates other difficulties, such as the loss of metabolon formation and intermediate diversion into other metabolic pathways. We show here that co-localization of these enzymes in the thylakoid membrane leads to a significant increase in product formation, with a concomitant decrease in off-pathway intermediates. This was achieved by exchanging the membrane anchors of the dhurrin pathway enzymes to components of the Twin-arginine translocation pathway, TatB and TatC, which have self-assembly properties. Consequently, we show 5-fold increased titers of dhurrin and a decrease in the amounts of intermediates and side products in Nicotiana benthamiana . Further, results suggest that targeting the UGT to the membrane is a key factor to achieve efficient substrate channeling. Highlights Fusion of Tat proteins to the enzymes of the dhurrin pathway in N. benthamiana leads to efficient substrate channeling. Cytochrome P450s are functional after exchange of membrane anchors with components of the Tat pathway. Targeting the UGT to the membrane is a key factor in substrate channeling. The Tat-fusions leads to a 5-fold increase in dhurrin production and decrease in intermediates and side-products.


  • 주제어

    Substrate channeling .   Twin-arginine translocation pathway .   Dhurrin .   Fusion proteins .   Light-driven .   Chloroplast.  

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