본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Metabolic engineering v.44, 2017년, pp.273 - 283   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Engineering photosynthetic production of L-lysine

Korosh, Travis C.    (Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States   ); Markley, Andrew L.    (Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States   ); Clark, Ryan L.    (Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States   ); McGinley, Laura L.    (Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States   ); McMahon, Katherine D.    (Environmental Chemistry and Technology Program, University of Wisconsin-Madison, Madison, WI 53706, United States   ); Pfleger, Brian F.    (Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States  );
  • 초록  

    Abstract L -lysine and other amino acids are commonly produced through fermentation using strains of heterotrophic bacteria such as Corynebacterium glutamicum . Given the large amount of sugar this process consumes, direct photosynthetic production is intriguing alternative. In this study, we report the development of a cyanobacterium, Synechococcus sp. strain PCC 7002, capable of producing L -lysine with CO 2 as the sole carbon-source. We found that heterologous expression of a lysine transporter was required to excrete lysine and avoid intracellular accumulation that correlated with poor fitness. Simultaneous expression of a feedback inhibition resistant aspartate kinase and lysine transporter were sufficient for high productivities, but this was also met with a decreased chlorophyll content and reduced growth rates. Increasing the reductant supply by using NH 4 + , a more reduced nitrogen source relative to NO 3 - , resulted in a two-fold increase in productivity directing 18% of fixed carbon to lysine. Given this advantage, we demonstrated lysine production from media formulated with a municipal wastewater treatment sidestream as a nutrient source for increased economic and environmental sustainability. Based on our results, we project that Synechococcus sp. strain PCC 7002 could produce lysine at areal productivities approaching that of sugar cane to lysine via fermentation using non-agricultural lands and low-cost feedstocks. Highlights Engineered lysine pathway of Synechococcus sp. strain PCC 7002 for secretion. Lysine exporter and deregulated aspartate kinase were the key steps. Upwards of 18% of fixed carbon was sent to lysine. Gravity belt filtrate from a municipal wastewater plant can be used as a N-source.


  • 주제어

    Cyanobacteria .   Synechococcus sp.   PCC 7002 .   Lysine .   Amino acids .   Metabolic engineering.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기