Hydrogen sulfide synthesis in native Saccharomyces cerevisiae strains during alcoholic fermentations
Abstract In order to diminish the undesirable impact of hydrogen sulfide (H 2 S) on wine, H 2 S synthesis was evaluated at phenotypic and transcriptional levels in 16 strains of Saccharomyces cerevisiae , which comprised 12 natural isolates, three commercial strains, and one laboratory heterozygote. Strain-dependent and multi-gene participation traits were evident, and high gene activity did not necessarily elevated H 2 S levels. When the variation of gene expression was analyzed between fermentation stages in each strain, similarities among some strains related to H 2 S formation. UCD522 and seven strains with low H 2 S production were grouped together based on cluster analysis and fold-change analysis. They displayed a negative relationship between activity of MET17 , HOM2 , SER33 and CYS4 and H 2 S formation, suggesting the role of biosynthesis of sulfur-containing amino acids. In the CECLFE1225, CECLFE1226 and UCD819 strains, transcriptional variation in MET3 , MET5 and MET10 might account for the changes in H 2 S amount. High levels of HOM2 and SER33 expression were implicated with the H 2 S phenotype of CECGM1 (H 2 S-free strain). MET1 may be a key gene in sulfide biosynthesis owing to its involvement in almost all strains. This study furthers the understanding of H 2 S formation in different S. cerevisiae strains and the industrial application of natural isolates. Highlights H 2 S synthesis in native S. cerevisiae strains was tested for industrial interest. H 2 S formation showed traits of strain-dependent and multi-gene participation. Synthesis of S-containing amino acid affected H 2 S formation in seven native strains. MET3 , MET5 and MET10 might account for H 2 S amount changes in two native strains.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기