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Journal of colloid and interface science v.512, 2018년, pp.14 - 20   SCI SCIE
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Adhesion force sensing and activation of a membrane-bound sensor to activate nisin efflux pumps in Staphylococcus aureus under mechanical and chemical stresses

Carniello, Vera    (Corresponding author at: Department of Biomedical Engineering (FB-40), University Medical Center Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands.  ); Harapanahalli, Akshay K.   Busscher, Henk J.   van der Mei, Henny C.  
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    Abstract Nisin-associated-sensitivity-response-regulator (NsaRS) in Staphylococcus aureus is important for its adhesion to surfaces and resistance against antibiotics, like nisin. NsaRS consists of an intra-membrane-located sensor NsaS and a cytoplasmatically-located response-regulator NsaR, which becomes activated upon receiving phosphate groups from the intra-membrane-located sensor. Hypothesis The intra-membrane location of the NsaS sensor leads us to hypothesize that the two-component NsaRS system not only senses “chemical” (nisin) but also “mechanical” (adhesion) stresses to modulate efflux of antibiotics from the cytoplasm. Experiments NsaS sensor and NsaAB efflux pump transcript levels in S. aureus SH1000 adhering to surfaces exerting different adhesion forces were compared, in presence and absence of nisin. Adhesion forces were measured using single-bacterial contact probe atomic force microscopy. Findings Gene expression became largest when staphylococci experienced strong adhesion forces combined with nisin-presence and the two-component NsaRS response to antibiotics was enhanced at a stronger adhesion force. This confirms that the intra-membrane-located sensor NsaS senses both chemical and mechanical stresses to modulate antibiotic clearance through the NsaAB efflux pump. This finding creates better understanding of the antibiotic resistance of bacteria adhering to surfaces and, in the fight against antibiotic-resistant pathogens, may aid development of advanced biomaterials on which bacterial efflux pumps are not activated. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Nisin .   NsaRS response-regulator .   Antibiotic susceptibility .   Biofilms .   Cell wall deformation .   Surface sensing .   Adhesion force .   Atomic force microscopy .   Gene expression.  

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