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Placenta v.60, 2017년, pp.1 - 8   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Vitamin D attenuates sphingosine-1-phosphate (S1P)-mediated inhibition of extravillous trophoblast migration

Westwood, Melissa (Maternal and Fetal Health Research Centre, Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK ) ; Al-Saghir, Khiria (Maternal and Fetal Health Research Centre, Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK ) ; Finn-Sell, Sarah (Maternal and Fetal Health Research Centre, Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK ) ; Tan, Cherlyn (Maternal and Fetal Health Research Centre, Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK ) ; Cowley, Elizabeth (Maternal and Fetal H ) ; Berneau, Stéphane ; Adlam, Daman ; Johnstone, Edward D. ;
  • 초록  

    Abstract Introduction Failure of trophoblast invasion and remodelling of maternal blood vessels leads to the pregnancy complication pre-eclampsia (PE). In other systems, the sphingolipid, sphingosine-1-phosphate (S1P), controls cell migration therefore this study determined its effect on extravillous trophoblast (EVT) function. Methods A transwell migration system was used to assess the behaviour of three trophoblast cell lines, Swan-71, SGHPL-4, and JEG3, and primary human trophoblasts in the presence or absence of S1P, S1P pathway inhibitors and 1,25(OH) 2 D 3 . QPCR and immunolocalisation were used to demonstrate EVT S1P receptor expression. Results EVTs express S1P receptors 1, 2 and 3. S1P inhibited EVT migration. This effect was abolished in the presence of the specific S1PR2 inhibitor, JTE-013 (p 2 D 3 for 48 or 72 h reduces S1PR2 (4-fold; 2 D 3 (p Discussion This study demonstrates that although EVT express three S1P receptor isoforms, S1P predominantly signals through S1PR2/Gα 12/13 to activate Rho and thereby acts as potent inhibitor of EVT migration. Importantly, expression of S1PR2, and therefore S1P function, can be down-regulated by vitamin D. Our data suggest that vitamin D deficiency, which is known to be associated with PE, may contribute to the impaired trophoblast migration that underlies this condition. Highlights Sphingosine-1-Phosphate (S1P) controls extravillous trophoblast (EVT) migration. Control of migration is exerted through Sphingosine-1-Phosphate receptor 2 (S1PR2). Vitamin D attenuates the expression of S1PR2 and therefore promotes EVT migration in the presence of S1P.


  • 주제어

    S1P .   Vitamin D .   GPCR .   Trophoblast .   Migration .   Lipid signalling .   Placenta .   Pre-eclampsia.  

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