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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072, 2018년, pp.397 - 404   SCI SCIE
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Multivariate optimization of solvent bar microextraction combined with HPLC-UV for determination of trace amounts of vincristine in biological fluids

Kiani, Mahsa (Active Pharmaceutical Ingredients Research Center (APIRC), Pharmaceutical Sciences Research Center, Medicinal Chemistry Department, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran-Iran ) ; Qomi, Mahnaz (Active Pharmaceutical Ingredients Research Center (APIRC), Pharmaceutical Sciences Research Center, Medicinal Chemistry Department, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran-Iran ) ; Hashemian, Farshad (Clinical Pharmacy Department, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran ) ; Rajabi, Mehdi (Clinical Pharmacy Department, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran ) ;
  • 초록  

    Abstract In the current work, an efficient method named solvent bar microextraction-high performance liquid chromatography-UV detection (HPLC-UV) was developed for preconcentration and determining the trace amount of vincristine (VCR) in biological samples such as plasma and urine. Briefly, VCR was extracted from an aqueous sample with pH 10.7 (donor phase) into 1-octanol as the supported liquid membrane (SLM) which is inserted into the pores of the hollow fiber and followed by back extraction into an aqueous receiving phase (pH=3.1). Studying the factors affecting the extraction performance in order to achieve a high extraction efficiency, requires the design of experiments (DOE) approach. In this regards, diverse factors’ effects including the pH value of donor and acceptor phases, extraction time, extraction temperature, stirring rate and salt content of the donor phase were considered. The optimum experimental condition was as following: pH of the source phase, 10.7; pH of the receiving phase, 3.1; stirring rate, 1000rpm; extraction temperature, 51°C; extraction time, 60min and 11.3% w/v NaCl in the sample solution. Under the optimal; extraction condition, a favorable preconcentration factor equal to 98.5 was achieved. The linearity range was obtained in the domain of 0.05–5mgL −1 . The limits of detection and quantification were 0.015 and 0.05mgL −1 . Within-day and between-day RSDs of the proposed SBME method were 4.1% and 12.5%, respectively. Finally, the applicability of the implemented SBME method was evaluated by the extraction and quantification of VCR from biological samples such as urine and plasma and satisfactory results were obtained. Highlights Trace amounts of vincristine were determined by SBME-HPLC-UV method. A multivariate optimization approach was applied to optimize extraction conditions. SBME method was simple, fast, and high efficient providing low LOD and high PF. The feasibility of the method was confirmed by analyzing plasma and urine samples.


  • 주제어

    Solvent bar microextraction .   Vincristine .   Biological fluids .   Preconcentration .   Design of experiment.  

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