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Diamond and related materials v.81, 2018년, pp.118 - 126   SCI SCIE
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Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces

Zakarienė, Gintarė (Department of Food Safety and Quality, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania ) ; Novoslavskij, Aleksandr (Department of Food Safety and Quality, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania ) ; Meškinis, Šarūnas (Institute of Materials Science, Kaunas University of Technology, K. Barsausko Str. 59, LT-51423 Kaunas, Lithuania ) ; Vasiliauskas, Andrius (Institute of Materials Science, Kaunas University of Technology, K. Barsausko Str. 59, LT-51423 Kaunas, Lithuania ) ; Tamulevičienė, Asta (Institute of Materials Science, Kaunas University of Technology, K. Barsausko Str. 59, LT-51423 Kaunas, Lithuania ) ; Tamulevičius, Sigitas (Institute of Materials Science, Kaunas University of Technology, K. Barsausko Str. 59, LT-51423 Kaunas, Lithuania ) ; Alter, Thomas (Institute of Food Safety and Food Hygiene, Freie Universitaet Berlin, Königsweg 69, 14163 Berlin, Germany ) ; Malakauskas, Mindaugas (Department of Food Safety and Quality, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kauna ) ;
  • 초록  

    Abstract The effect of thin (5nm) and thick (40nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C . jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22at.% Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06log 10 CFU/ml after 15min and 3.61log 10 CFU/ml after 30min on DLC:Ag coated silicon wafers in comparison to control samples (P≤0.05). L. monocytogenes was not detected on DLC:Ag samples after 24h of exposure (P≤0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments. Highlights Culturable C. jejuni and L. monocytogenes numbers are reduced by DLC:Ag surfaces. DLC:Ag surfaces induce the formation of VBNC form of C. jejuni and L. monocytogenes . DLC:Ag could reduce cross-contamination risk for C. jejuni during food preparation. DLC:Ag antimicrobial surface could reduce L. monocytogenes persistence. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Campylobacter jejuni .   Listeria monocytogenes .   DLC Ag nanocomposites.  

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