본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Analytica chimica acta : an international journal devoted to all branches of analytical chemistry v.994, 2017년, pp.49 - 55   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry

Lee, Hsun (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Lai, Yin-Hung (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Ou, Yu-Meng (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Tsao, Chien-Wei (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Jheng, Ya-Jin (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Kuo, Shu-Yun (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ; Chang, Huan-Tsung (Chemistry Department, National Taiwan University, Taipei 106, Taiwan, ROC ) ; Wang, Yi-Sheng (Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC ) ;
  • 초록  

    Abstract Carbohydrate analysis is challenging due to lack of sensitive detection and efficient separation methods. Although matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a sensitive tool, the low ionization efficiency of carbohydrates makes mass analyses inefficient. This work systematically examines the correlation between MALDI-MS sensitivity and carbohydrate sample morphology. Depending on the properties of the matrix used, the morphology changes through sample recrystallization after drying or imposition of hydrodynamic flows during droplet drying. Observation shows that amorphous solids and finer crystals offer higher carbohydrate sensitivity and spatial homogeneity than larger crystals. Clear evidences of an inverse correlation between sensitivity and crystal size are obtained when various kinds of carbohydrates are mixed with different matrixes. Similar experiments on proteins and peptides showed a negative or negligible effect. The result serves as a general guideline for improving efficiency in routine carbohydrate analysis. Highlights Carbohydrate sensitivity systematically increases as MALDI crystal size decrease. Methanol recrystallization efficiently produces fine and amorphous sample crystal to provide enhanced sensitivity. Accelerated Marangoni flow precisely controls sample crystal size for sensitivity optimization. The improvement is effective for various carbohydrates and matrixes. Methanol recrystallization and accelerated Marangoni flow do not change protein sensitivity. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Carbohydrate .   Sample morphology .   Crystalline structure .   Sensitivity .   Matrix-assisted laser desorption/ionization.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기