본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Stem cell reports v.10 no.2, 2018년, pp.461 - 476   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells

Ngondo, Richard Patryk    (Swiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, Zurich, Switzerland   ); Cirera-Salinas, Daniel    (Swiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, Zurich, Switzerland   ); Yu, Jian    (Swiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, Zurich, Switzerland   ); Wischnewski, Harry    (Swiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, Zurich, Switzerland   ); Bodak, Maxime    (Swiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, Zurich, Switzerland   ); Vandormael-Pournin, Sandrine    (Institut Pasteur, CNRS, Unité  ); Geiselmann, Anna    (de Génétique Fonctionnelle de la Souris, UMR 3738, Department of Developmental & Stem Cell Biology, 25 rue du docteur Roux, 75015 Paris Cedex, France   ); Wettstein, Rahel    (Institut Pasteur, CNRS, Unité  ); Luitz, Janina    (de Génétique Fonctionnelle de la Souris, UMR 3738, Department of Developmental & Stem Cell Biology, 25 rue du docteur Roux, 75015 Paris Cedex, France   ); Cohen-Tannoudji, Michel    (Swiss Federal Institute of Technology Zurich  ); Ciaudo, Constance  
  • 초록  

    Summary In mouse, although four Argonaute (AGO) proteins with partly overlapping functions in small-RNA pathways exist, only Ago2 deficiency causes embryonic lethality. To investigate the role of AGO2 during mouse early development, we generated Ago2 -deficient mouse embryonic stem cells (mESCs) and performed a detailed characterization of their differentiation potential. Ago2 disruption caused a global reduction of microRNAs, which resulted in the misregulation of only a limited number of transcripts. We demonstrated, both in vivo and in vitro, that AGO2 is dispensable for the embryonic germ-layer formation. However, Ago2 -deficient mESCs showed a specific defect during conversion into extra-embryonic endoderm cells. We proved that this defect is cell autonomous and can be rescued by both a catalytically active and an inactive Ago2, but not by Ago2 deprived of its RNA binding capacity or by Ago1 overexpression. Overall, our results suggest a role for AGO2 in stem cell differentiation. Highlights • Ago2 deletion strongly affects microRNA but not mRNA levels in mESCs • AGO2 is dispensable for mESC self-renewal and formation of embryonic germ layers • AGO2 but not AGO1 is required for GATA6 expression during XEN conversion of mESCs • AGO2 is essential for the in vitro expression of extra-embryonic endoderm genes Ngondo et al. report that Ago2 is required for the extra-embryonic endoderm (ExEn) differentiation in vitro . While AGO2 is dispensable for proper embryo formation, its deletion in mESCs causes a decreased induction of ExEn genes, leading to an impaired differentiation. This defect is cell autonomous and involves AGO2 small-RNA binding capacity.


  • 주제어

    Argonaute 2 .   mouse embryonic stem cells .   extra-embryonic endoderm .   XEN cells .   miRNA .   differentiation .   transcriptomics.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기