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Sensors and actuators. B, Chemical v.259, 2018년, pp.162 - 169   SCI SCIE SCOPUS
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Plasmonic ELISA for naked-eye detection of ochratoxin A based on the tyramine-H2O2 amplification system

Liang, Yi (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China ) ; Huang, Xiaolin (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China ) ; Chen, Xirui (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China ) ; Zhang, Wenjing (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China ) ; Ping, Guo (Jiangxi Food Inspection and Testing Research Institute, Nanchang, 330096, PR China ) ; Xiong, Yonghua (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China ) ;
  • 초록  

    Abstract A novel direct competitive plasmonic enzyme-linked immunosorbent assay (dc-pELISA) was applied to detect ochratoxin A (OTA) with naked eyes. In this assay, horseradish peroxidase (HRP) + hydrogen peroxide (H 2 O 2 ) + tyramine (TYR)-induced gold nanoparticle (AuNP) aggregation was considered as a signal output; AuNP aggregation could be triggered through the phenol polymerization of TYR, which was induced by hydroxyl radicals from HRP-catalyzed H 2 O 2 ; OTA-labeled catalase (CAT) was used as a competing antigen to consume H 2 O 2 . Owing to the combined advantages of ultrahigh CAT catalytic activity for H 2 O 2 and dual-color responses (red and blue) generated through AuNP aggregation, the proposed method was highly sensitive and thus could be employed with naked eyes to detect OTA qualitatively with a cut-off limit of 150 pg/mL. Our method also demonstrated a good dynamic linear range (12.5–150 pg/mL) for quantitative OTA determination with a reliable correlation coefficient of R 2 = 0.992, a half-maximal inhibitory concentration of 84.75 pg/mL, and a detection limit of 17.8 pg/mL. In brief, this newly-designed technique is considerably suitable for high-throughput screening detection or point-of-care diagnostics in resource-constrained regions because of the easy readout of results by naked eyes without the use of advanced detection equipment. Highlights HRP + H 2 O 2 + TYR-induced AuNP aggregation was firstly considered as a signal output of dc-pELISA for OTA detection. The cut-off limit of developed dc-pELISA was achieved at 150 pg/mL by naked eye. This proposed strategy is considerably suitable for high-throughput screening detection or point-of-care diagnostics. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Immunoassay .   Ochratoxin A .   Hydrogen peroxide .   Gold nanoparticle aggregation .   Tyramine.  

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