DNA-templated Au nanoclusters and MnO2 sheets: a label-free and universal fluorescence biosensing platform
Abstract A label-free and universal fluorescence biosensing paltform has been constructed for probing and recognizing biomolecular interactions based on the excellent fluorescent properties of poly(adenine) (poly A) DNA templated-Au nanoclusters (DNA-AuNCs) and the good quenching ability of MnO 2 sheets. Due to the high quenching efficiency of MnO 2 sheets, the luminescence of DNA-AuNCs was decreased. Because single-stranded DNA-AuNCs was adsorbed onto the surface of MnO 2 sheets through the physical absorption behavior between nucleobases of single-stranded DNA and the basal plane of MnO 2 sheets. When the target DNA was introduced, the DNA-AuNCs could hybridize with the target DNA and form double-stranded duplex DNA structures, which resulting in the desorption of DNA-AuNCs from the surface of MnO 2 sheets. Thus, the fluorescence signal of system was recovered. Similarly, in the presence of target substrate, aptamer-substrate complexes formed and resulted in the luminescence of DNA-AuNCs. By exploring DNA-AuNCs as signal reporter and MnO 2 sheets as the quencher, this strategy could avoid the complex labelling process of the DNA probe and offer the advantages of simplicity and cost efficiency. Highlights We developed a new fluorescence sensing platform for probing biomolecular. Low-cost MnO 2 sheets were used as novel fluorescent quencher. DNA templated AuNCs were utilized for fluorescent indicator and molecular recognition. This strategy was free of any fluorescence dye label and other complex design. The combination of DNA-AuNCs and MnO 2 sheets brings novel opportunities in bioanalysis. Graphical abstract A novel fluorescent biosensing paltform has been developed for probing and recognizing biomolecular interactions on the basis of the excellent fluorescent properties of DNA-AuNCs and the good quenching ability of MnO 2 sheets. [DISPLAY OMISSION]
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