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Sensors and actuators. B, Chemical v.259, 2018년, pp.307 - 315   SCI SCIE SCOPUS
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Biocompatible silica nanoparticles conjugated with azidocoumarin for trace level detection and visualization of endogenous H2S in PC3 cells

Rajalakshmi, Kanagaraj (Green City Technology Institute, Korea Institute of Science & Technology, Hwarang-ro 14-gil 5 SeongBuk-gu, Seoul 02792, Republic of Korea ) ; Nam, Yun-Sik (Advanced Analysis Center, Korea Institute of Science & Technology, Hwarang-ro 14-gil 5 SeongBuk-gu, Seoul 02792, Republic of Korea ) ; Youg, Sumin (Green City Technology Institute, Korea Institute of Science & Technology, Hwarang-ro 14-gil 5 SeongBuk-gu, Seoul 02792, Republic of Korea ) ; Selvaraj, Muthusamy (Green City Technology Institute, Korea Institute of Science & Technology, Hwarang-ro 14-gil 5 SeongBuk-gu, Seoul 02792, Republic of Korea ) ; Lee, Kang-Bong (Green City Technology Institute, Korea Institute of Science & Technology, Hwarang-ro 14-gil 5 SeongBuk-gu, Seoul 02792, Republic of Korea ) ;
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    Abstract The silica nanoparticles (SiO 2 NPs) conjugated with azidocoumarin (Cy-N 3 ) was applied for the selective and sensitive H 2 S detection, even when H 2 S was present in 1000-fold excess of other bio thiols and ions. In the silica nanoprobes, azide in the Cy-N 3 group acted as the recognition element, and the probe was biocompatible. The nanoprobe displayed blue emission at 456 nm and excitation at 367 nm. After introducing H 2 S into the probe solution, the fluorescent intensity at 456 nm increased linearly as a function of H 2 S concentration (correlation coefficient, 0.9970), and the limit of detection was 6 nM. The enhanced fluorescent emission intensity was due to H 2 S-mediated reduction of the electron-withdrawing azide into electron-donating amine. The SiO 2 NPs@Cy-N 3 and its reaction products with H 2 S (SiO 2 NPs@Cy-NH 2 ) were characterized by 1 H NMR and TEM. The probe also showed low cytotoxicity and could trace endogenous H 2 S in physiological pH. We therefore then successfully utilized it to detect H 2 S in living PC3 cells. The probe was also successfully employed for detection of H 2 S gas using Whatman papers coated with SiO 2 NPs@Cy-N 3 . Highlights Biocompatible chemosensor for H 2 S detection was developed using silica nanoparticles. H 2 S detection was achieved even in 1000-fold excess of other interferents. The lowest LOD was 6 nM under physiological conditions. The probe was successfully applied for H 2 S detection in live PC3 cells. The paper strip probe coated with silica nanoparticles successfully detected H 2 S. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Silica nanoparticle .   Azidocoumarin .   H2S in living cells .   H2S assay .   Selective H2S detection.  

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