본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Sensors and actuators. B, Chemical v.259, 2018년, pp.573 - 579   SCI SCIE SCOPUS
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Cross-priming amplification-based lateral flow strip as a novel tool for rapid on-site detection of wild-type pseudorabies virus

Meng, Xing-Yu (Corresponding author. ) ; Gao, Yao ; Zhang, Huawei ; Luo, Yuzi ; Sun, Yuan ; Qiu, Hua-Ji ;
  • 초록  

    Abstract Pseudorabies (PR), also called as Aujeszky’s disease, is an important infectious disease of pigs and other mammals caused by pseudorabies virus (PRV). This paper presents a novel assay based on cross-priming amplification in combination with gold nanoparticle lateral flow strip (CAP-strip) for rapid and on-site detection of wild-type PRV. In the assay, FITC and biotin were labeled at the 5′-ends of the two probes, respectively, resulting in amplified products labeled with either FITC or biotin at the 5′-end. Then the amplified products (biotin-dsDNA-FITC), as novel targets, were recognized by standardized strips. The assay had excellent visualization results from 2.0 × 10 2 to 2.0 × 10 8 copies of PRV and the detection limit was determined to be 200 copies. No cross-reactivity was observed with several common porcine viruses. When testing a total of 293 field swine samples, the agreement between the CPA-strip assay and a triplex real-time PCR or virus isolation were 100% (293/293) or 97.3% (285/293). Collectively, the assay is specific, sensitive and convenient that enables rapid, on-site diagnosis of PRV infections. Highlights Cross-priming amplification-based lateral-flow strip was developed for detection of wild-type pseudorabies virus. The assay combined the sensitivity of cross-priming amplification with the convenience of the strip. The labeled amplicons were detected by standardized strips. The assay was able to detect as low as 200 copies of the virus. The assay can be used for rapid, on-site diagnosis of pseudorabies.


  • 주제어

    Cross-priming amplification .   Lateral-flow strip .   On-site detection .   Pseudorabies virus.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기