본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Sensors and actuators. B, Chemical v.259, 2018년, pp.809 - 815   SCI SCIE SCOPUS
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Alkaline phosphatase detection using electrochemical impedance of anti-alkaline phosphatase antibody (Ab354) functionalized silicon-nanowire-forest in phosphate buffer solution

Mintz Hemed, Nofar (Dept. of Physical Electronics, Eng. Faculty, and the Univ. Res. Inst. for Nano Science and Nano-Technologies, Tel-Aviv University, Ramat-Aviv 69978, Israel ) ; Convertino, Annalisa (Istituto per la Microelettronica e i Microsistemi C.N.R.-Area della Ricerca di Roma, via del Fosso del Cavaliere 100, I-00133 Roma, Italy ) ; Shacham-Diamand, Yosi (Dept. of Physical Electronics, Eng. Faculty, and the Univ. Res. Inst. for Nano Science and Nano-Technologies, Tel-Aviv University, Ramat-Aviv 69978, Israel ) ;

  • 초록  

    Abstract This article presents an electrochemical impedance study on the detection of Alkaline Phosphatase (ALP) in phosphate buffer saline (PBS) solution by using anti-ALP functionalized Si nanowires (SiNWs). Electrochemical impedance was measured using a three-electrode cell where the working electrode was a very dense and highly disordered array of Si NWs, e.g. SiNW-forest. This array was grown using plasma enhanced chemical vapor deposition (PECVD) at 350 °C. The NW surface was modified by immobilizing the anti-ALP. A very efficient detection of very low ALP concentrations in PBS solution, ranging from 0.03 U/L to 0.3 U/L (1 nM–10 nM respectively), as well as a high selectivity due to the antibody/antigen interaction were demonstrated. The remarkable sensitivity and selectivity achieved through the combination of SiNW-forest combined electrochemical impedance spectroscopy suggest an effective strategy for real time and quantitative sensing of ALP. Highlights This study presents an electrochemical method for Alkaline Phosphatase (ALP) detection based on anti-ALP functionalized Si nanowire platform. The ability to sense between different concentrations of ALP has been demonstrated. The detection limit of 1 nM is one of thelowest demonstrated by electrochemical methods and the lowest demonstrated by impedancespectroscopy. Control and specificity measurements prove that blocking exists, and excellent specificity was achieved. Highly reproducibility of the system was achieved.


  • 주제어

    Si nanowire .   Si nanowire-forest .   Electrochemical impedance spectroscopy .   Biosensor .   Alkaline phosphatase.  

 활용도 분석

  • 상세보기

    amChart 영역

  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기