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Sensors and actuators. B, Chemical v.259, 2018년, pp.402 - 410   SCI SCIE SCOPUS
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Encapsulated cholesterol oxidase in metal-organic framework and biomimetic Ag nanocluster decorated MoS2 nanosheets for sensitive detection of cholesterol

Hassanzadeh, Javad (Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran ) ; Khataee, Alireza (Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran ) ; Eskandari, Habibeh (Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran ) ;
  • 초록  

    Abstract Herein, a nano-porous metal-organic-framework (MOF) was applied as a safe host for cholesterol oxidase (ChOx) enzyme to improve its stability and implement an efficient oxidation process for substrate molecules. Subsequently, a fluorometric method based on the conversion of non-fluorescent terephthalic acid (TA) to 2-Hydroxyterephthalate with a great fluorescence emission was used for fluorescence detection of the generated H 2 O 2 . On the other hand, Ag nanocluster (AgNC) decorated MoS 2 nanosheets (MoS 2 -NS) nanocomposite as a new mimetic peroxidase catalyst was exploited in TA-H 2 O 2 reaction. The synthesized enzyme encapsulated MOF and AgNC/MoS 2 -NS nanocomposites were characterized using scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction and some other analyzing techniques. The results showed a high porous MOF with a high capacity to uptake ChOx enzymes, improving their catalytic activity. The ChOx-MOF hybrid displayed good efficiency for oxidation of cholesterol. Also, an improved peroxidase-like activity was discovered for AgNC/MoS 2 -NS composite, in comparison with each of the constituents or natural peroxidase enzyme (HRP). As a result, the performance of designed cholesterol probe based on ChOx-MOF and peroxidase-like AgNC/MoS 2 -NS, was improved significantly, in which it presented a dynamic linear range of 0.06 μM–15 μM with a detection limit (3S) of 0.03 μM. The studied method showed a good selectivity and sensitivity for practical determination of cholesterol and its potential application for cholesterol detection in human blood samples. Highlights Use of porous metal-organic-framework (MOF) as a safe host for cholesterol oxidase. High stability of enzymes in MOF pores and good efficiency for cholesterol oxidation. Reaction of created H 2 O 2 and terephthalic acid to form fluorescent hydroxyterephthalate. Use of Ag nanocluster decorated MoS 2 nanosheets hybrid as a new peroxidase catalyst. High sensitive cholesterol probe based on ChOx/MOF and new peroxidase-like catalyst. Graphical abstract Cholesterol oxidase encapsulated in MOF-5 and subsequent H 2 O 2 -terephthalic acid reaction in the presence of Ag nanocluster/MoS 2 nanosheets catalyst was used for cholesterol determination. [DISPLAY OMISSION]


  • 주제어

    Non-enzyme mimetic .   Enzyme capsulated MOF .   MoS2 nanosheets .   Ag nanocluster .   Composite .   Cholesterol.  

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