Effects of copper sulphate (CuSO4) elicitation on the chemical constitution of volatile compounds and the in vitro development of Basil
Abstract Basil produces important compounds for the industrial manufacture of medicines, cosmetics, and food that are obtained by conventional production techniques. In vitro cultivation has contributed to the increasing reliability of the production, and efficient isolation, of target compounds independent of seasonal factors. We sought to assess the effects of copper sulfate on seedling growth and the elicitation of chemical constituents of the essential oil of basil leaves cultivated in vitro . Basil seedlings were grown in Murashige and Skoog (MS) medium enriched with 25 and 75 μM CuSO 4 , as well as in control treatments. Essential oils obtained from the hydrodistillation of dried leaves were subjected to GC–MS analysis. The GC–MS and PCA analyses indicated the presence of two predominant volatile groups: Phenylpropanoids (77.81%) in the 25 μM CuSO 4 treatment, with eugenol and methyl eugenol as major components, with industrial and pharmacological importance; and Monoterpenes (69.72%), in the 75 μMCuSO 4 treatment and control, subdivided into monoterpene hydrocarbons (19.36%) and oxygenated monoterpenes (50.36%), with 1,8 cineole and linalool as major components.MS medium enriched with 25 μM CuSO 4 resulted in seedlings with longer and more numerous leaves, which would be important for oil extraction. That Cu concentration probably affected the phenylpropanoid metabolic pathway, which is triggered mainly under plant stress situations. Highlights The use of 25 μM CuSO 4 clearly increased the production of phenylpropanoids (77.81%). The use of 25 μM CuSO 4 clearly increased the components methyl eugenol (41.05%) and eugenol (36.37%). Some compounds were only encountered in the essential oil of basil leaves cultivated with 25 μM CuSO 4 . The addition of CuSO 4 25 μm to the MS medium promoted increased shoot height and greater numbers of leaves.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.
NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.
- 이 논문과 함께 출판된 논문 + 더보기