본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Phytochemistry v.149, 2018년, pp.116 - 122   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Biochemical characterization of microbial type terpene synthases in two closely related species of hornworts, Anthoceros punctatus and Anthoceros agrestis

Xiong, Wangdan (Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA ) ; Fu, Jianyu (Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China ) ; Köllner, Tobias G. (Department of Biochemistry, Max Planck Institute for Chemical Ecology, Germany ) ; Chen, Xinlu (Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA ) ; Jia, Qidong (Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA ) ; Guo, Haobo (College of Engineering & Computer Science, SimCenter, University of Tennessee, Chattanooga, TN 37403, USA ) ; Qian, Ping (Shandong Agricultural University, Chemistry and Material Science Faculty, Tai'an 271018, Shandong, China ) ; Guo, Hong (Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA ) ; Wu, Guojiang (South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China ) ; Chen, Feng (Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA ) ;
  • 초록  

    Abstract Microbial terpene synthase-like ( MTPSL ) genes are a type of terpene synthase genes only recently identified in plants. In contrast to typical plant terpene synthase genes, which are ubiquitous in land plants, MTPSL genes appear to occur only in nonseed plants. Our knowledge of catalytic functions of MTPSLs is very limited. Here we report biochemical characterization of the enzymes encoded by MTPSL genes from two closely related species of hornworts, Anthoceros punctatus and Anthoceros agrestis . Seven full-length MTPSL genes were identified in A. punctatus ( ApMTPSL1 - 7 ) based on the analysis of its genome sequence. Using homology-based cloning, the apparent orthologs for six of the ApMTPSL genes, except ApMTPSL2 , were cloned from A. agrestis . They were designated AaMTPSL1, 3–7 . The coding sequences for each of the 13 Anthoceros MTPSL genes were cloned into a protein expression vector. Escherichia coli -expressed recombinant MTPSLs from hornworts were assayed for terpene synthase activities. Six ApMTPSLs and five AaMTPSLs, except for ApMTPSL5 and AaMTPSL5, showed catalytic activities with one or more isoprenyl diphosphate substrates. All functional MTPSLs exhibited sesquiterpene synthase activities. In contrast, only ApMTPSL7 and AaMTPSL7 showed monoterpene synthase activity and only ApMTPSL2, ApMTPSL6 and AaMTPSL6 showed diterpene synthase activity. Most MTPSLs from Anthoceros contain uncanonical aspartate-rich motif in the form of either ‘DDxxxD’ or ‘DDxxx’. Homology-based structural modeling analysis of ApMTPSL1 and ApMTPSL7, which contain ‘DDxxxD’ and ‘DDxxx’ motif, respectively, showed that ‘DDxxxD’ and ‘DDxxx’ motifs are localized in the similar positions as the canonical ‘DDxxD’ motif in known terpene synthases. To further understand the role of individual aspartate residues in the motifs, ApMTPSL1 and ApMTPSL7 were selected as two representatives for site-directed mutagenesis studies. No activities were detected when any of the conserved aspartic acid was mutated into alanine. This study provides new information about the catalytic functions of MTPSLs and the functionality of their uncanonical aspartate-rich motifs, and builds a knowledge base for studying the biological importance of MTPSL genes and their terpene products in nonseed plants. Highlights Hornworts Anthocerus punctatus and Anthocerus agrestis contain MTPSL genes. Most Anthocerus MTPSL genes encode active terpene synthases. The uncanonical aspartate-rich motifs in MTPSLs are important for catalysis. Graphical abstract The hornworts Anthoceros punctatus and Anthoceros agrestis each contains a small family of microbial-terpene synthase like ( MTPSL ) genes. Recombinant proteins expressed in E. coli from most Anthoceros MTPSL genes showed sesquiterpene synthase activities, converting farnesyl diphosphate to a few to nearly 20 sesquiterpenes. [DISPLAY OMISSION]


  • 주제어

    Anthoceros punctatus .   Anthoceros agrestis .   Anthocerotaceae .   MTPSL .   Specialized metabolism .   Nonseed plants.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기