본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Mutation research. Fundamental and molecular mechanisms of mutagenesis v.808, 2018년, pp.1 - 9   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage

Aybastıer, Önder (University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059 Bursa, Turkey ) ; Dawbaa, Sam (University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059 Bursa, Turkey ) ; Demir, Cevdet (University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059 Bursa, Turkey ) ; Akgün, Oğuzhan (University of Uludag, Faculty of Science and Arts, Department of Biology, 16059 Bursa, Turkey ) ; Ulukaya, Engin (University of İstinye, Faculty of Medicine, Department of Medical Biochemistry, İstanbul, Turkey ) ; Arı, Ferda (University of Uludag, Faculty of Science and Arts, Department of Biology, 16059 Bursa, Turkey ) ;
  • 초록  

    Abstract Lung cancer has a high treatment cost and poor prognosis in comparison to other types of cancers. This work was involved in studying oxidative DNA base damage inhibition. Accordingly, standard carvacrol, thymol, thymoquinone with water and water-methanol extract of thyme ( Origanum vulgare L. subsp . hirtum (link.) Ietswaart), thyme oil and thyme water were prepared and investigated for their efficacy to inhibit DNA oxidative damage formed by H 2 O 2 in malignant lung cells (A549). The antioxidant capacity by ABTS assay was 271.73 ± 11.45 mg trolox equivalent/mL for thyme oil. HPLC analysis was carried out to determine the contents of different thyme extracts, results showing the presence of carvacrol, thymol, protocatechuic acid, caffeic acid, epicatechin and rosmarinic acid in water and water-methanol extracts while only carvacrol and thymol were found in thyme oil and thyme water. After DNA isolation from the cultured cells, the formed oxidative induced DNA damage products were analysed using GC–MS/MS. It was proven that the antioxidants in the cell culture media have succeeded to inhibit oxidative DNA base damage. Thymoquinone was shown to be the best protectant antioxidant among other antioxidants against the formation of oxidative DNA damage, whereas water-methanol extract of thyme was the best among the plant-sourced samples. Thymoquinone and thyme water-methanol extract were investigated for their efficacy on cultured healthy lung cells (BEAS-2B), and it was proven that they are efficient in protection against the oxidation of DNA of healthy lung cells too. Highlights Antioxidants and plant extracts were investigated for preventing DNA damage. Oxidative damage to DNA was studied in healthy and malignant lung cells. Carvacrol, thymol, thymoquinone and thyme extracts were the targeted objects. Antioxidants particularly thymoquinone impeded the DNA damage products formation. Thyme extracts particularly water-methanol extract impeded the damage too. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Oxidative DNA damage .   Antioxidant .   Thyme .   Lung cancer .   GC–MS/MS.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기