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Myocardial infarction-induced hippocampal microtubule damage by cardiac originating microRNA-1 in mice

Sun, Lin-Lin (Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China ) ; Duan, Ming-Jing (Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China ) ; Ma, Ji-Chao (Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China ) ; Xu, Ling (Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China ) ; Mao, Meng (Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China ; ) ; Biddyut, Das ; Wang, Qin ; Yang, Chao ; Zhang, Shuai ; Xu, Yi ; Yang, Lin ; Tian, You ; Liu, Ying ; Xia, Sheng-Nan ; Li, Ke-Xin ; Jin, Zhuo ; Xiong, Qiaojie ; Ai, Jing ;
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    Abstract Cardiovascular diseases are risk factors for dementia, but the mechanisms remain elusive. Here, we report that myocardial infarction (MI) generated by the ligation of the left coronary artery (LCA) could lead to increased miR-1 levels in the hippocampus and blood with neuronal microtubule damage and decreased TPPP/p25 protein expression in the hippocampus. These changes could be prevented by a knockdown of miR-1 using hippocampal stereotaxic injections of anti- miR-1 oligonucleotide fragments carried by a lentivirus vector (lenti-pre-AMO- miR-1 ). TPPP/p25 protein was downregulated by miR-1 overexpression, upregulated by miR-1 inhibition, and unchanged by binding-site mutations or miR-masks, indicating that the TPPP/p25 gene was a potential target for miR-1 . Additionally, the pharmacological inhibition of sphingomyelinase by GW4869 to inhibit exosome generation in the heart significantly attenuated the increased miR-1 levels in the hippocampi of transgenic (Tg) and MI mice. Collectively, the present study demonstrates that MI could directly lead to neuronal microtubule damage independent of MI-induced chronic brain hypoperfusion but involving the overexpression of miR-1 in the hippocampus that was transported by exosomes from infarcted hearts. This study reveals a novel insight into the molecular mechanisms of heart-to-brain communication at the miRNA level. Highlights Myocardial infarction leads to miR-1 overexpression in hippocampus. Exosome transports miR-1 from the heart to brain. Myocardial infarction induces neuronal microtubule damage in hippocampus. miR-1 results neuronal microtubule damage by downregulating TPPP/p25 protein. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    MicroRNA-1 .   Myocardial infarction .   Brain .   Neuronal microtubule .   TPPP/p25 .   Exosome.  

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