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Codon usage revisited: Lack of correlation between codon usage and the number of tRNA genes in enterobacteria

Rojas, Joaquín (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) ; Castillo, Gabriel (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) ; Leiva, Lorenzo Eugenio (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) ; Elgamal, Sara (Department of Microbiology and The Center for RNA Biology, Ohio State University, Columbus, OH, 43210, USA ) ; Orellana, Omar (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) ; Ibba, Michael (Department of Microbiology and The Center for RNA Biology, Ohio State University, Columbus, OH, 43210, USA ) ; Katz, Assaf (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) ;
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    Abstract It is widely believed that if a high number of genes are found for any tRNA in a rapidly replicating bacteria, then the cytoplasmic levels of that tRNA will be high and an open reading frame containing a higher frequency of the complementary codon will be translated faster. This idea is based on correlations between the number of tRNA genes, tRNA concentration and the frequency of codon usage observed in a limited number of strains as well as from the fact that artificially changing the number of tRNA genes alters translation efficiency and consequently the amount of properly folded protein synthesized. tRNA gene number may greatly vary in a genome due to duplications, deletions and lateral transfer which in turn would alter the levels and functionality of many proteins. Such changes are potentially deleterious for fitness and as a result it is expected that changes in tRNA gene numbers should be accompanied by a modification of the frequency of codon usage. In contrast to this model, when comparing the number of tRNA genes and the frequency of codon usage of several Salmonella enterica and Escherichia coli strains we found that changes in the number of tRNA genes are not correlated to changes in codon usage. Furthermore, these changes are not correlated with a change in the efficiency of codon translation. These results suggest that once a genome gains or loses tRNA genes, it responds by modulating the concentrations of tRNAs rather than modifying its frequency of codon usage. Highlights Average number of tRNA genes correlate with codon usage in highly expressed genes. Changes in the number of tRNA genes do not correlate to codon usage. Altered tRNA gene copy numbers do not correlate to changes of codon translation. Our data indicates that tRNA gene copies adapt to codon usage, but not the contrary.


  • 주제어

    tRNA .   Codon usage .   Efficiency of translation .   Enterobacteria .   Salmonella enterica .   Escherichia coli.  

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