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Fish & shellfish immunology v.79, 2018년, pp.1 - 10   SCI SCIE
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In vitro effects of Origanum vulgare leaf extracts on gilthead seabream (Sparus aurata L.) leucocytes, cytotoxic, bactericidal and antioxidant activities

Beltrán, José (Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain ) ; María García (Università ) ; Espinosa, Cristóbal (degli Studi di Palermo, Dipartimento di Scienze della terra e del Mare DiSTeM, Laboratorio di Biochimica Marina ed Ecotossicologia, Via G. Barlotta 4, 91100 Trapani, Italy ) ; Guardiola, Francisco A. (Fish Nutrition & Immunobiology Group, Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixoes, Av. General Norton de Matos s/n, 4450-208 Porto, Portugal ) ; Esteban, M. Ángeles (Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain ) ;
  • 초록  

    Abstract Origanum vulgare is a well-known medicinal plant that has been used since ancient times as an additive in foods and cosmetic preparations. The possible application of O. vulgare extracts in fish was assessed by using gilthead seabream ( Sparus aurata ) as a marine fish model due to its importance in aquaculture. The in vitro effects of aqueous and ethanolic leaf extracts of O. vulgare were tested in order to observe any immunostimulant, cytotoxic, bactericidal or antioxidant properties. The results showed that medium or high concentration of aqueous extracts and low concentrations of ethanolic extract, increased head kidney leucocyte activities as well as the number of SAF-1 cells. However, moderate to high concentrations of ethanolic extracts decreased both leucocyte activities and the number of viable SAF-1 cells, suggesting some possible toxic effect towards them. Only the highest concentration of the aqueous extract and medium to high concentrations of the ethanolic extracts showed cytotoxic activity against the tumor PLHC-1 cell line. Bactericidal activity was only detected against Vibrio harveyi, V. anguillarum and Photobacterium damselae when using the highest concentration of aqueous extract and moderate to high concentrations of ethanolic extract. Finally, both plant extracts presented antioxidant activity particularly the aqueous extract. Overall, the results suggest that both extracts (when used at the appropriate concentration) have immunostimulant, cytotoxic, bactericidal and antioxidant properties, making O. vulgare an interesting candidate for incorporation as additive in functional diets for farmed fish. Highlights Oregano aqueous and ethanolic extracts had immunostimulant activities on gilthead seabream HK leucocytes. Aqueous and ethanolic extracts had cytotoxic activities on SAF-1 and PHLC-1 cell lines. Ethanolic extracts had more bactericidal activity than aqueous ones. Aqueous extracts had more antioxidant activity than ethanolic extracts. Graphical abstract [DISPLAY OMISSION]


  • 주제어

    Origanum vulgare .   Leave extracts .   Immunostimulants .   Cytotoxicity .   Bactericidal activity .   Antioxidants .   Gilthead seabream (Sparus aurata L.   ) .   Teleosts .   Aquaculture.  

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