Silencing of a Kazal-type serine proteinase inhibitor SPIPm2 from Penaeus monodon affects YHV susceptibility and hemocyte homeostasis
Abstract In shrimp, the Kazal-type serine proteinase inhibitors (KPIs) are involved in host innate immune defense system against pathogenic microorganisms. A five-Kazal-domain SPI Pm 2 is the most abundant KPIs in the black tiger shrimp Penaeus monodon and up-regulated in response to yellow head virus (YHV) infection. In this study, the role of SPI Pm 2 in YHV infection was investigated. The expression of SPI Pm 2 in hemocytes, gill and heart from 48-h YHV-infected shrimp was increased. The expression of SPI Pm 2 in hemocytes was significantly increased after 12 h of infection and gradually increased higher afterwards. Silencing of SPIPm2 by dsRNA interference resulted in the increased expression of different apoptosis-related genes, the increased expression of transcriptional factors of antimicrobial synthesis pathways, the reduction of circulating hemocytes in the shrimp hemolymph, and the increased susceptibility of the silenced shrimp to YHV infection. The activities of caspase-3 and caspase-7 in the hemocytes of SPIPm2 -silenced shrimp was also increased by 5.32-fold as compared with those of the control shrimp. The results suggested that the SPI Pm 2 was involved in the hemocyte homeostasis. Highlights The SPIPm2 gene is up-regulated in response to YHV infection. The SPIPm2 -silencing results in up-regulation of and apoptosis-related genes. The SPIPm2 silencing results in a decrease in the number of circulating hemocytes. The caspase activities are increased in SPIPm2 -silenced shrimp. The SPIPm2 -silenced shrimp are more sensitive to YHV.
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