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Nature chemistry v.10 no.6, 2018년, pp.631 - 637   SCI SCIE
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I-motif DNA structures are formed in the nuclei of human cells

Zeraati, Mahdi (Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia ) ; Langley, David B. (St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Kensington, Sydney, New South Wales, Australia ) ; Schofield, Peter (Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia ) ; Moye, Aaron L. (Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia ) ; Rouet, Romain (Children's Medical Research Institute, University of Sydney, Westmead, Sydney, New South Wales, Australia ) ; Hughes, William E. (Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia ) ; Bryan, Tracy M. (Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia ) ; Dinger, Marcel E. (St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Kensington, Sydney, New South Wal ) ; Christ, Daniel ;
  • 초록  

    Human genome function is underpinned by the primary storage of genetic information in canonical B-form DNA, with a second layer of DNA structure providing regulatory control. I-motif structures are thought to form in cytosine-rich regions of the genome and to have regulatory functions; however, in vivo evidence for the existence of such structures has so far remained elusive. Here we report the generation and characterization of an antibody fragment (iMab) that recognizes i-motif structures with high selectivity and affinity, enabling the detection of i-motifs in the nuclei of human cells. We demonstrate that the in vivo formation of such structures is cell-cycle and pH dependent. Furthermore, we provide evidence that i-motif structures are formed in regulatory regions of the human genome, including promoters and telomeric regions. Our results support the notion that i-motif structures provide key regulatory roles in the genome.


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