본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of cellular physiology v.233 no.10, 2018년, pp.7036 - 7046  

Isolation and identification of chemotherapy‐enriched sphere‐forming cells from a patient with gastric cancer

Bagheri, Vahid (School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran ) ; Memar, Bahram (Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ) ; Behzadi, Ramezan (Msc North research Center‐Pasture Institute of Iran, North Research Center‐Pasture Institute of Iran, Amol, Iran ) ; Aliakbarian, Mohsen (Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ) ; Jangjoo, Ali (Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ) ; Bahar, Mostafa Mehrabi (Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ) ; Talebi, Samaneh (Immunology Research Center, Mashhad U ) ; Gholamin, Mehran ; Abbaszadegan, Mohammad R. ;
  • 초록  

    Gastric cancer (GC) is the third and fifth cause of cancer‐associated mortality for men and women throughout the world, respectively. Despite the use of surgery and chemotherapy for GC therapy, there are no efficient therapeutic protocols for it to date. Cancer stem cells (CSCs) due to their pivotal role in tumor initiation, growth, progression, invasion, distant metastasis, recurrence and resistance to anticancer drugs are very appealing targets for cancer therapies. Here, we isolated and identified CSCs from a chemotherapy‐treated patient. Small subpopulation of dissociated cells after tissue digestion formed spheroid colonies in serum‐free media under the non‐adherent condition. These spheroid colonies differentiated into epithelial like cells in serum‐containing medium. Few sphere‐forming cells carried CD44 and CD54 markers overexpressed DLL4 that is responsible for tumor growth and angiogenesis. Subcutaneous injections of sphere‐forming cells in different passages conferred tumorigenicity in nude mice. Sphere‐forming cells upregulated CD44 polymorphisms CD44v3, ‐v6, and ‐v8 ‐10, stemness factors OCT4, SOX2, SALL4 and Cripto‐1, self‐renewal molecules IHh, Wnt, β‐catenin and BMI1, and epithelial mesenchymal transition (EMT) markers Twist1 and Snail1 in vitro and in vivo. Moreover, these cells similar to sphere‐forming cells isolated from a chemotherapy‐free patient expressed Oct‐4 and β‐catenin proteins. However, the Twist1 protein was only expressed by sphere‐forming cells derived from the chemotherapy‐treated patient. Thus, these cells have all the characteristics of stationary and migratory CSCs, including tumorigenicity, self‐renewal, pluripotency, invasion and metastasis. Taken together, targeting chemotherapy‐enriched CSCs as chemo‐resistance cells observed in GC patients can provide more effective therapeutic strategies compared to untreated patients.


  • 주제어

    cancer stem cells .   chemotherapy resistance .   sphere .   sphere‐forming cells.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기