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Journal of cellular physiology v.233 no.10, 2018년, pp.6921 - 6928  

Notch activation promotes osteoblast mineralization by inhibition of apoptosis

Xu, Ying (Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China ) ; Shu, Bing (Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China ) ; Tian, Ye (Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, China ) ; Chelly, Marjorie (Department of Orthopaedic Surgery, Louisiana State University Health Sciences Center, Shreveport, Louisiana ) ; Morandi, Massimo M. (Department of Orthopaedic Surgery, Louisiana State University Health Sciences Center, Shreveport, Louisiana ) ; Barton, Shane (Department of Orthopaedic Surgery, Louisiana State University Health Sciences Center, Shreveport, Louisiana ) ; Shang, Xifu (Department of Orthopaedic Surgery, Anhui Provincial Hospital, Hefei, Anhui, China ) ; Dong, Yufeng (Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China ) ;
  • 초록  

    Notch activator Jagged1 (JAG1) plays a critical role in the regulation of osteoblast differentiation and bone metabolism. In this study, JAG1‐induced osteoblast proliferation, differentiation, and mineralization has been analyzed in primary osteoblasts for up to 7 days. Alkaline phosphatase and Alizarin red staining showed an enhanced osteoblast maturation and mineralization in JAG1 treated cells, as well as higher mRNA levels of late osteoblast differentiation markers. In contrast, Notch inhibitor DAPT and deletion of Runx2 totally blocked JAG1 effects on osteoblast mineralization. Flow cytometry data further showed a significantly higher cell proliferation in early stages of culture at day 3, and lower levels of osteoblast apoptosis in late stages of culture at day 7. More importantly, activation of anti‐apoptotic factor BCL‐2 was enhanced, while pro‐apoptotic factor Caspase3 was reduced in JAG1 treated osteoblasts. Therefore, we conclude that cell mineralization is enhanced via anti‐apoptotic actions of Notch signaling within the osteoblast cells.


  • 주제어

    apoptosis .   notch signaling .   osteoblast differentiation.  

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