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Macromolecular bioscience v.18 no.7, 2018년, pp.1700390 -   

TGF‐β1‐Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

Böck, Thomas (Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Str. 6, 97080, Würzburg, Germany ) ; Schill, Verena (Department of Functional Materials for Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany ) ; Krähnke, Martin (Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Str. 6, 97080, Würzburg, Germany ) ; Steinert, Andre F. (Orthopedic Center for Musculoskeletal Research, University of Würzburg, Brettreichstr. 11, 97074, Würzburg, Germany ) ; Tessmar, Jörg (Department of Functional Materials for Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany ) ; Blunk, Torsten (Department of Trauma, Hand, Plasti ) ; Groll, Jürgen ;
  • 초록  

    Abstract In cartilage regeneration, the biomimetic functionalization of hydrogels with growth factors is a promising approach to improve the in vivo performance and furthermore the clinical potential of these materials. In order to achieve this without compromising network properties, multifunctional linear poly(glycidol) acrylate (PG‐Acr) is synthesized and utilized as crosslinker for hydrogel formation with thiol‐functionalized hyaluronic acid via Michael‐type addition. As proof‐of‐principle for a bioactivation, transforming growth factor‐beta 1 (TGF‐β1) is covalently bound to PG‐Acr via Traut's reagent which does not compromise the hydrogel gelation and swelling behavior. Human mesenchymal stromal cells (MSCs) embedded within these bioactive hydrogels show a distinct dose‐dependent chondrogenesis. Covalent incorporation of TGF‐β1 significantly enhances the chondrogenic differentiation of MSCs compared to hydrogels with supplemented noncovalently bound TGF‐β1. The observed chondrogenic response is similar to standard cell culture with TGF‐β1 addition with each medium change. In general, multifunctional PG‐Acr offers the opportunity to introduce a range of biomimetic modifications (peptides, growth factors) into hydrogels and, thus, appears as an attractive potential material for various applications in regenerative medicine.


  • 주제어

    chondrogenesis .   hyaluronic acid .   mesenchymal stromal cells .   poly(glycidol) .   TGF‐β1.  

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