본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Phycological research v.66 no.3, 2018년, pp.199 - 208   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Characterization of Porphyra lucasii pigment mutant induced by gamma irradiation

Lee, Hak‐Jyung (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, South Korea ) ; Choi, Jong‐il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, South Korea ) ; Kim, Gwang H. (Department of Biology, Kongju National University, Kongju, South Korea ) ;
  • 초록  

    The present study describes the isolation of a pigment mutant strain of Porphyra lucasii, which was obtained using gamma irradiation. Gametophytic blades of this seaweed were irradiated with gamma rays at doses of 500 and 1000Gy. After irradiation, red color was observed in the blades exposed to the 500Gy dose. Single somatic cells from these irradiated blades were isolated from archespores and then cultured to obtain pigment mutant blades that are referred to as 500GR in this investigation. Evaluation of genetic variations in these blades was performed using random amplification of polymorphic DNA (RAPD) analysis. Chlorophyll content, antioxidant activity, and total phenolic content were found to be higher in 500GR than in the wild type (WT) algal strain. Protein extracts obtained from the blades were examined using 2D gel electrophoresis; changes in the proteome were finally analyzed using MS/MS. A total of 216 proteins were detected. From these, nine proteins were over-expressed in the pigment spots in 500GR as compared to WT. These results indicate that the over-expressed proteins are either antioxidant enzymes or photosynthesis-related proteins.


  • 주제어

    antioxidant enzymes .   gamma irradiation .   pigment mutant .   Porphyra lucasii .   proteomic analysis.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기