본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Soci v.24 no.3, 2018년, pp.450 - 458   SCI SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Glycan distribution and density in native skin's stratum corneum

Danzberger, J. (Center for Advanced Bioanalysis GmbH, Linz, Austria ) ; Donovan, M. (L'Oréal Research and Innovation, Aulnay sous Bois, France ) ; Rankl, C. (RECENDT‐Research Center for Non‐Destructive Testing GmbH, Linz, Austria ) ; Zhu, R. (Institute for Biophysics, Johannes Kepler University Linz, Linz, Austria ) ; Vicic, S. (L'Oréal Research and Innovation, Aulnay sous Bois, France ) ; Baltenneck, C. (L'Oréal Research and Innovation, Aulnay sous Bois, France ) ; Enea, R. (L'Oréal Research and Innovation, Aulnay sous Bois, France ) ; Hinterdorfer, P. (Center for Advanced Bioanalysis GmbH, Linz, Austria ) ; Luengo, G. S. (L'Oréal Research and Innovation, Aulnay sous Bois, France ) ;
  • 초록  

    Abstract Background The glycosylation of proteins on the surface of corneocytes is believed to play an important role in cellular adhesion in the stratum corneum (SC) of human skin. Mapping with accuracy the localization of glycans on the surface of corneocytes through traditional methods of immunohistochemistry and electron microscopy remains a challenging task as both approaches lack enough resolution or need to be performed in high vacuum conditions. Materials and methods We used an advanced mode of atomic force microscope (AFM), with simultaneous topography and recognition imaging to investigate the distribution of glycans on native (no chemical preparation) stripped samples of human SC. The AFM cantilever tips were functionalized with anti‐heparan sulfate antibody and the lectin wheat germ agglutinin (WGA) which binds specifically to N‐acetyl glucosamine and sialic acid. Results From the recognition imaging, we observed the presence of the sulfated glycosaminoglycan, heparan sulfate, and the glycans recognized by WGA on the surface of SC corneocytes in their native state. These glycans were found associated with bead‐like domains which represent corneodesmosomes in the SC layers. Glycan density was calculated to be ~1200 molecules/μm 2 in lower layers of SC compared to an important decrease, (~106 molecules/μm 2 ) closer to the surface due probably to corneodesmosome degradation. Conclusion Glycan spatial distribution and degradation is first observed on the surface of SC in native conditions and at high resolution. The method used can be extended to precisely localize the presence of other macromolecules on the surface of skin or other tissues where the maintenance of its native state is required.


  • 주제어

    antibody .   atomic force microscopy .   heparan sulfate .   molecular recognition .   stratum corneum .   topography and recognition imaging .   wheat germ agglutinin.  

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기