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The Plant journal : for cell and molecular biology v.95 no.3, 2018년, pp.516 - 528   SCI SCIE
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LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce (Lactuca sativa L.)

Chen, Zijing (Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193, China ) ; Zhao, Wensheng (Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193, China ) ; Ge, Danfeng (Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 201602, China ) ; Han, Yingyan (Plant Science and Technology College, Beijing University of Agriculture/New Technological Laboratory in Agriculture Application in Beijing, Beijing, 102206, China ) ; Ning, Kang (Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193, ) ; Luo, Chen ; Wang, Shenglin ; Liu, Renyi ; Zhang, Xiaolan ; Wang, Qian ;
  • 초록  

    Summary Lettuce ( Lactuca sativa L.) is one of the most economically important vegetables. The floral transition in lettuce is accelerated under high temperatures, which can significantly decrease yields. However, the molecular mechanism underlying the floral transition in lettuce is poorly known. Using laser capture microdissection coupled with RNA sequencing, we isolated shoot apical meristem cells from the bolting‐sensitive lettuce line S39 at four critical stages of development. Subsequently, we screened specifically for the flowering‐related gene LsSOC1 during the floral transition through comparative transcriptomic analysis. Molecular biology, developmental biology, and biochemical tools were combined to investigate the biological function of LsSOC1 in lettuce. LsSOC1 knockdown by RNA interference resulted in a significant delay in the timing of bolting and insensitivity to high temperature, which indicated that LsSOC1 functions as an activator during heat‐promoted bolting in lettuce. We determined that two heat shock transcription factors, HsfA1e and HsfA4c , bound to the promoter of LsSOC1 to confirm that LsSOC1 played an important role in heat‐promoted bolting. This study indicates that LsSOC1 plays a crucial role in the heat‐promoted bolting process in lettuce. Further investigation of LsSOC1 may be useful for clarification of the bolting mechanism in lettuce.


    Significance Statement The SOC1 homologous gene from lettuce, LsSOC1 , has a specific function in heat‐promoting bolting. Two heat‐shock transcription factors, LsHsfA1e and LsHsfA4c , bind to the LsSOC1 promoter to activate bolting.


  • 주제어

    lettuce .   laser capture microdissection .   RNA‐seq .   LsSOC1 .   heat‐promoted.  

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