본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression

Wang, Yuebing (Department of Biochemistry, School of Medicine, Nankai University, Tianjin 300071, China ) ; Shao, Guoqiang (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Wu, Jianping (Department of Urology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Cui, Can (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Zang, Shimin (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Qiu, Fan (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Jia, Ruipeng (Department of Urology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Wang, Zizheng (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ; Wang, Feng (Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China ) ;
  • 초록  

    Objective. To synthesize 68 Ga-Glu-urea-Lys(Ahx)-HBED-CC ( 68 Ga-PSMA-11) with a synthesis module and investigate PET-CT imaging to monitor PSMA expression during prostate cancer (PCa) progression and tumor growth in mice bearing subcutaneous PCa xenografts. Method. The radiochemical purity and stability of 68 Ga-PSMA-11 were determined via radio-HPLC. The PCa cell lines of different PSMA expression levels (PC3, VCAP±, CWR22RV1+, and LNCaP++) were selected to mimic the PCa progression. 68 Ga-PSMA-11 biodistribution was studied by dissection method and in vivo imaging with micro PET-CT. The expression levels of PSMA in tumor cells and tissues were analyzed by immunofluorescence, flow cytometry, and western blot. The correlation between PSMA expression and radio-uptake was also evaluated. 2-PMPA preadministration served as a block group. Results. The radiochemical purity of 68 Ga-PSMA-11 was 99.6 ± 0.1% and stable in vitro for 2 h. The equilibrium binding constant (Kd) of 68 Ga-PSMA-11 to LNCaP, CWR22Rv1, PC-3, and VCAP cells was 4.3 ± 0.8 nM, 16.4 ± 1.3 nM, 225.3 ± 20.8 nM, and 125.6 ± 13.1 nM, respectively. Results of tumor uptake (% ID and % ID/g or % ID/cm 3 ) of 68 Ga-PSMA-11 in biodistribution and micro PET imaging were LNCaP > CWR22RV1 > PC-3 and VCAP due to different PSMA expression levels. It was confirmed by flow cytometry, western blot, and immunofluorescence. Tumor uptake (% ID/cm 3 ) of 68 Ga-PSMA-11 increased with the tumor anatomical volume in quadratic polynomial fashion and reached the peak (when tumor volume was 0.5 cm 3 ) earlier than tumor uptake (% ID). Tumor uptake (% ID/cm 3 ) of 68 Ga-PSMA-11 based on functional volume correlated well with the PSMA expression in a linear manner (y=9.35x+2.59, R2 =0 0.8924, and p 68 Ga-PSMA-11. Conclusions. The 68 Ga-PSMA-11 PET-CT imaging could invasively evaluate PSMA expression during PCa progression and tumor growth with % ID/cm 3 (based on functional volume) as an important index. Low dose 2-PMPA preadministration might be a choice to decrease kidney uptake of 68 Ga-PSMA-11.


 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기