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Anaerobe 22건

  1. [해외논문]   Editorial Board   SCI SCIE


    Anaerobe v.51 ,pp. ii - ii , 2018 , 1075-9964 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  2. [해외논문]   Improving culture media for the isolation of Clostridium difficile from compost   SCI SCIE

    Dharmasena, Muthu (Corresponding author.) , Jiang, Xiuping
    Anaerobe v.51 ,pp. 1 - 7 , 2018 , 1075-9964 ,

    초록

    Abstract This study was to optimize the detection methods for Clostridium difficile from the animal manure-based composts. Both autoclaved and unautoclaved dairy composts were inoculated with a 12-h old suspension of a non-toxigenic C. difficile strain (ATCC 43593) and then plated on selected agar for vegetative cells and endospores. Six types of enrichment broths supplemented with taurocholate and L -cysteine were assessed for detecting a low level of artificially inoculated C. difficile (ca. 5 spores/g) from dairy composts. The efficacy of selected enrichment broths was further evaluated by isolating C. difficile from 29 commercial compost samples. Our results revealed that using heat-shock was more effective than using ethanol-shock for inducing endospore germination, and the highest endospore count (p C. difficile agar base, supplemented with 0.1% L -cysteine, 7% defibrinated horse blood, and cycloserine-cefoxitin (CDA-CYS-H-CC agar) was the best medium (p C. difficile endospore populations from both types of composts enumerated on both CDA-CYS-H-CC agar supplemented with 0.1% sodium taurocholate (CDA-CYS-H-CC-T agar) and brain heart infusion agar supplemented with 0.5% yeast extract, 0.1% L -cysteine, cycloserine-cefoxitin, and 0.1% sodium taurocholate (BHIA-YE-CYS-CC-T agar) media were not significantly different from each other (p > 0.05). Overall, enrichment of inoculated compost samples in broths containing moxalactam-norfloxacin (MN) produced significantly higher (p C. difficile from environmental samples with high background microflora. Highlights Sub-lethal heat treatment is more effective in inducing C. difficile endospores. Pre-reduced media with 0.1% L -cysteine recover C. difficile cells in 24 h. Solid media with L -cysteine and taurocholate recover more C. difficile endospores. BHIB-YE-CYS-MN-T broth yields the highest recovery of C. difficile from compost.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Brain abscess and cervical lymphadenitis due to Paraclostridium bifermentans: A report of two cases   SCI SCIE

    Sankar R, Udhaya (Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India ) , Biswas, Rakhi (Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India ) , Raja, S. (Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India ) , Sistla, Sujatha (Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India ) , Gopalakrishnan, M.S. (Department of Neurosurgery, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India ) , Saxena, Sunil Kumar (Department of Ear, Nose and Throat, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India)
    Anaerobe v.51 ,pp. 8 - 11 , 2018 , 1075-9964 ,

    초록

    Abstract Paraclostridium bifermentans (current nomenclature of Clostridium bifermentans since 2016) is a gram-positive, spore-forming anaerobic bacterium. Here, we describe two cases associated with this organism. The first, primarily a case of tubercular brain abscess where P. bifermentans was isolated as part of a polymicrobial flora, following a neurosurgical procedure for the same and the second, a case of cervical lymphadenitis from which it was isolated as the sole causative agent. There are only a few reported cases of P. bifermentans in literature and these cases illustrate the widening spectrum of infections related to it. Highlights First case report of cervical lymphadenitis due to Paraclostridium bifermentans . Paraclostridium bifermentans isolated from a case of brain abscess, as a part of polymicrobial causation. Pathogenic potential of Paralostridium bifermentans needs to be evaluated.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   Evaluation of the Cepheid® Xpert® C. difficile binary toxin (BT) diagnostic assay   SCI SCIE

    McGovern, Alan M. (School of Biomedical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia ) , Androga, Grace O. (School of Biomedical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia ) , Moono, Peter (School of Biomedical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia ) , Collins, Deirdre A. (School of Medical and Health Sciences, Edith Cowan University, Joondalup, Western Australia, Australia ) , Foster, Niki F. (OzFoodNet, Communicable Disease Control Directorate, Department of Health, Government of Western Australia, East Perth, Western Australia, Australia ) , Chang, Barbara J. (School of Biomedical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia ) , Riley, Thomas V. (Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia)
    Anaerobe v.51 ,pp. 12 - 16 , 2018 , 1075-9964 ,

    초록

    Abstract Strains of Clostridium difficile producing only binary toxin (CDT) are found commonly in animals but not humans. However, human diagnostic tests rarely look for CDT. The Cepheid Xpert C. difficile BT assay detects CDT with equal sensitivity (≥92%) in human and animal faecal samples. Highlights Clostridium difficile binary toxin (CDT) is not detected by most diagnostic tests. The Cepheid Xpert C. difficile BT assay reports CDT regardless of other toxins. The assay is equally sensitive (≥92%) in both human and animal samples.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Acute septic arthritis of the knee: A rare case report of infection with Parvimonas micra after an intra-articular corticosteroid injection for osteoarthritis   SCI SCIE

    Sultan, Assem A. (Corresponding author. Department of Orthopedic Surgery, Cleveland Clinic, 9500 Euclid Avenue/A40, Cleveland, OH, 44195, USA.) , Cantrell, William A. , Khlopas, Anton , Cole, Connor , Piuzzi, Nicolas S. , Sodhi, Nipun , Brooks, Peter , Mont, Michael A.
    Anaerobe v.51 ,pp. 17 - 20 , 2018 , 1075-9964 ,

    초록

    Abstract Acute bacterial mono-articular septic arthritis affects most commonly the lower extremity joints in adult population and most commonly caused by Staphylococcus aureus . Various risk factors determine susceptibility to infection including host immunity, medical co-morbidity and joint structural abnormality and other organisms may be involved. Parvimonas micra ( P. micra ) is among the rare organisms that may be associated with atypical septic arthritis and primarily affects the native knee joint. Only 3 case reports in the literature have reported on pyogenic pyogenic joint infection caused by this organism. We hereby present our experience with a case of knee septic arthritis caused by P. micra in a patient with knee osteoarthritis following a recent intra-articular injection with corticosteroids. In susceptible patients, Intra-articular corticosteroid injection and crystal induced arthritis may propose a potential risk of contracting infection with P. micra . Highlights A case report on native knee joint septic arthritis caused by rare anaerobic organism, Parvimonas Micra . Atypical clinical presentation of septic arthritis is highlighted through this case. Potential risk factors highlighted in this case is intra-articular injection with corticosteroids and the concurrent presence of calcium pyrophosphate crystals in the joint. Review of previous case reports and identified important clinical pearls are also discussed.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   Presence of Clostridium difficile in poultry and poultry meat in Egypt   SCI SCIE

    Abdel-Glil, Mostafa Y. (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96A, 07743 Jena, Germany ) , Thomas, Prasad (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96A, 07743 Jena, Germany ) , Schmoock, Gernot (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96A, 07743 Jena, Germany ) , Abou-El-Azm, Kamel (Department of Poultry Diseases, Faculty of Veterinary Medicine, Manusoura University, Egypt ) , Wieler, Lothar H. (Institute of Microbiology and Epizootics, Department of Veterinary Medicine, Freie Universität, Robert-von-Ostertag-Str. 7-13, Building 35, 14163, Berlin, Germany ) , Neubauer, Heinrich (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96A, 07743 Jena, Germany ) , Seyboldt, Christian (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Str. 96A, 07743 Jena, Germany)
    Anaerobe v.51 ,pp. 21 - 25 , 2018 , 1075-9964 ,

    초록

    Abstract C. difficile has been recognized as a potential zoonotic agent encouraging investigations of C. difficile prevalence and ribotypes in animals. Here we report the prevalence and diversity of Egyptian C. difficile in I) samples from healthy poultry (n = 50), II) samples from diseased poultry (n = 54), and III) poultry meat (n = 150). Thirteen isolates were obtained from seven healthy and five diseased animals, but no C. difficile was cultured from poultry meat. The isolated C. difficile strains belonged to 3 different PCR-ribotypes (039/2, 205 and 001/FLI01). The detection of strains related to RT 001 known for its ability to cause disease in humans makes poultry a potential reservoir for pathogenic C. difficile . Highlights 104 poultry samples and 150 meat samples from Egypt were investigated for C. difficile . C. difficile prevalence in birds was 11.5% (12 of 104), 14% (7 of 50) for healthy and 9.3% (5 of 54) for diseased poultry. Three different PCR-ribotypes RT 039/2, RT 205 and RT 001/FLI01 were found. C. difficile was not found in poultry meat samples.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   Species-specific serine-threonine protein kinase Pkb2 of Bifidobacterium longum subsp. longum: Genetic environment and substrate specificity   SCI SCIE

    Nezametdinova, V.Z. (Corresponding author. Laboratory of Genetics of Microorganisms, Vavilov Institute of General Genetics Russian Academy of Sciences, 3 Gubkin Street, 119991, GSP-1, Moscow, Russian Federation.) , Mavletova, D.A. , Alekseeva, M.G. , Chekalina, M.S. , Zakharevich, N.V. , Danilenko, V.N.
    Anaerobe v.51 ,pp. 26 - 35 , 2018 , 1075-9964 ,

    초록

    Abstract The objective of this study was to determine for phosphorylated substrates of the species-specific serine-threonine protein kinase (STPK) Pkb2 from Bifidobacterium longum subsp. longum GT15. Two approaches were employed: analyses of phosphorylated membrane vesicles protein spectra following kinase reactions and analyses of the genes surrounding pkb2 . A bioinformatics analysis of the genes surrounding pkb2 found a species-specific gene cluster PFNA in the genomes of 34 different bifidobacterial species. The identified cluster consisted of 5–8 genes depending on the species. The first five genes are characteristic for all considered species. These are the following genes encoding serine-threonine protein kinase ( pkb2 ), fibronectin type III domain-containing protein ( fn3 ), AAA-ATPase ( aaa-atp ), hypothetical protein with DUF58 domain ( duf58 ) and transglutaminase ( tgm ). The sixth (protein phosphatase, prpC ), seventh (hypothetical protein, BLGT_RS02790), and eighth (FHA domain-containing protein, fha ) genes are included in this cluster, but they are not found in all species. The operon organization of the PFNA gene cluster was confirmed with transcriptional analysis. AAA-ATPase, which is encoded by a gene of the PFNA gene cluster, was found to be a substrate of the STPK Pkb2. Fourteen AAA-ATPase sites (seven serine, six threonine, and one tyrosine) phosphorylated by STPK Pkb2 were revealed. Analysis of the spectra of phosphorylated membrane vesicles proteins allowed us to identify eleven proteins that were considered as possible Pkb2 substrates. They belong to several functional classes: proteins involved in transcription and translation; proteins of the F1-domain of the FoF1–ATPase; ABC-transporters; molecular chaperone GroEL; and glutamine synthase, GlnA1. All identified proteins were considered moonlighting proteins. Three out of 11 proteins (glutamine synthetase GlnA1 and FoF1-ATPase alpha and beta subunits) were selected for further in vitro phosphorylation assays and were shown to be phosphorylated by Pkb2. Four phosphorylated substrates of the species-specific STPK Pkb2 from B. longum subsp. longum GT15 were identified for the first time. They included the moonlighting protein glutamine synthase GlnA, FoF1-ATPase alpha and beta subunits, and the chaperone MoxR family of AAA-ATPase. The ability of bifidobacterial STPK to phosphorylate the substrate on serine, threonine, and tyrosine residues was shown for the first time. Highlights Genetic environment and substrates of STPK Pkb2 from B. longum were investigated. The Pkb2 operon was detected in the genomes of 34 different bifidobacterial species. Four phosphorylated substrates of the Pkb2 were identified for the first time. Pkb2 phosphorylated the substrate on serine, threonine, and tyrosine residues.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   The detection and prevalence of leukotoxin gene variant strains of Fusobacterium necrophorum in footrot lesions of sheep in Kashmir, India   SCI SCIE

    Farooq, S. (KVK-Kupwara, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Jammu and Kashmir, India ) , Wani, S.A. (Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India ) , Hassan, M.N. (KVK-Nyoma, SKUAST-K, Jammu and Kashmir, India ) , Aalamgeer, S. (Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India ) , Kashoo, Z.A. (Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India ) , Magray, S.N. (Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India ) , Bhat, M.A. (Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), R.S.Pura, Jammu and Kashmir, India)
    Anaerobe v.51 ,pp. 36 - 41 , 2018 , 1075-9964 ,

    초록

    Abstract The objective of this study was to determine the prevalence and identification of leukotoxin gene, lktA, variant strains of Fusobacterium necrophorum in the footrot lesions of sheep. The detection of F. necrophorum was carried out by PCR targeting the lktA gene fragment and identification of lktA variant strains was done by PCR–single stranded conformational polymorphism (PCR-SSCP) and gene sequencing. Of the 450 swabs collected from footrot lesions of sheep, 117 were lktA- positive for F. necrophorum . Of the 50 swabs collected from apparently asymptomatic sheep, only one was lktA- positive for F. necrophorum. The overall prevalence of F. necrophorum in footrot affected sheep in Kashmir valley was 26%, and ranged from 20 to 34.8%, respectively. PCR-SSCP of lktA gene fragment analysis revealed three lktA variants, designated as JKS-F1/F2/F3, while two samples (1.7%) showed multiple lktA variant strains of F. necrophorum in a single footrot-affected sheep hoof. This appears to be the first report on the presence of more than one lktA variant of F. necrophorum in a footrot lesion of sheep. The JKS-F3 lktA variant was the most frequent (75.4%), followed by JKS-F2 (14.4%) and JKS-F1 (8.4%), respectively. Among the three lktA variants identified, JKS-F3 was detected in 74 (86.0%) samples from severe footrot affected sheep with a lesion score of 4. The data suggest that JKS-F3 is the predominant lktA variant of F. necrophorum and is associated with severe footrot in sheep. Hence, JKS-F3 may be a significant variant contributing to the severity and duration of the disease in sheep. Highlights The overall prevalence of F. necrophorum in footrot lesions of sheep in Kashmir valley was 26%. Polymerase chain reaction - single stranded conformational polymorphism (PCR-SSCP) analysis of lktA gene fragment revealed the presence of three lktA variants of F. necrophorum , designated as JKS-F1/F2/F3. The JKS-F3 was the most frequent (75.4%), followed by JKS-F2 (14.4%) and JKS-F1 (8.4%) lktA variant, respectively. The study also revealed presence of multi-strains of F. necrophorum in a single footrot affected sheep hoof.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   High prevalence of Clostridium difficile PCR ribotype 078 in pigs in Korea   SCI SCIE

    Kim, Ha-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea ) , Cho, Ara (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea ) , Kim, Jong Wan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea ) , Kim, Heejung (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea ) , Kim, Bumseok (Laboratory of Pathology, College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea)
    Anaerobe v.51 ,pp. 42 - 46 , 2018 , 1075-9964 ,

    초록

    Abstract Clostridium difficile can cause neonatal enteritis, but has been isolated from both diseased and healthy pigs. C. difficile shedding by pigs is a potential source of zoonotic transmission to humans. The objectives of this study were to characterize the prevalence and genotype of C. difficile with respect to age and health status in the pig industry for the first time in Korea. Fecal samples of 910 pigs were analyzed for the presence of C. difficile . In total, 176 (19.3%) C. difficile strains were isolated. The prevalence was significantly higher ( p tcdA, tcdB, cdtA, and cdtB were all detected in 153 isolates (A + B + CDT + , 86.9%). Furthermore, the majority of toxigenic strains (86.5%) were identified as PCR ribotype 078, which is an important pathogen in both pigs and humans. These results indicated that C. difficile is a leading causative pathogen of neonatal diarrhea in piglets in Korea. Highlights This study is the first large-scale analysis of Clostridium difficile in pigs in Korea. Most isolates were toxigenic with binary toxin. Korean pigs are frequently colonized with PCR ribotype 078.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  10. [해외논문]   Type C botulism in domestic chickens, dogs and black-pencilled marmoset (Callithrix penicillata) in Minas Gerais, Brazil   SCI SCIE

    Silva, Rodrigo Otá (Corresponding author.) , vio Silveira , Martins, Ronaldo Alves , Assis, Ronnie Antunes , Oliveira Junior, Carlos Augusto , Lobato, Francisco Carlos Faria
    Anaerobe v.51 ,pp. 47 - 49 , 2018 , 1075-9964 ,

    초록

    Abstract Botulism is a well-known intoxication that affects humans and animals. The disease is endemic in cattle in Brazil and recently emerged as an important disease in commercial laying hens and broiler chickens in Europe. Dogs and other animal species can also be affected. Although antitoxins are commonly administered to humans diagnosed with botulism, in animals this is rarely the case and the treatment of botulism is still based only on support therapy. In the present work, we report an outbreak of type C botulism in Brazil that simultaneously affected domestic chickens, dogs and a black-pencilled marmoset ( Callithrix penicillata ). The successful use of Clostridium botulinum types C and D antitoxin for the treatment of an affected dog is also described.

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    원문보기
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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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