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General and comparative endocrinology 15건

  1. [해외논문]   CO2: Editorial Board  


    General and comparative endocrinology v.255 ,pp. IFC , 2018 , 0016-6480 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   CO2: Editorial Board   SCI SCIE


    General and comparative endocrinology v.255 ,pp. IFC - IFC , 2018 , 0016-6480 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   VIH from the mud crab is specifically expressed in the eyestalk and potentially regulated by transactivator of Sox9/Oct4/Oct1   SCI SCIE

    Liu, Chunyun (Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China ) , Jia, Xiwei (Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China ) , Zou, Zhihua (Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China ) , Wang, Xiaowei (Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China ) , Wang, Yilei (Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China ) , Zhang, Ziping (College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
    General and comparative endocrinology v.255 ,pp. 1 - 11 , 2018 , 0016-6480 ,

    초록

    Abstract Vitellogenesis-inhibiting hormone (VIH) is known to regulate ovarian maturation by suppressing the synthesis of vitellogenin (Vtg) in crustaceans, which belongs to a member of crustacean hyperglycemic hormone (CHH) family synthesized and secreted from the X-organ/sinus gland complex of eyestalks. In this study, the cDNA, genomic DNA (gDNA) and the 5′-upstream regulatory (promoter region) sequences of VIH gene were obtained by conventional PCR, genome walker and tail-PCR techniques according to our transcriptomic database of Scylla paramamosain . The full-length cDNA of SpVIH is 634bp including 105bp 5′UTR, 151bp 3′UTR and 378bp ORF that encodes a peptide of 125 amino acids. The full length gDNA of SpVIH is 790bp containing two exons and one intron. The 5′-flanking promoter regions of SpVIH we isolated are 3070bp from the translation initiation (ATG) and 2398bp from the predicted transcription initiation (A), which consists of putative core promoter region and multiple potential transcription factor binding sites. SpVIH was only expressed in eyestalk. The expression level of SpVIH in eyestalk of female crab decreased gradually along with the development of ovary. As there is not cell line of crabs available, we chose the mature transfection system HEK293FT cell lines to explore the mechanism of transcription regulation of SpVIH in crabs. Sequential deletion assays using luciferase reporter gene in HEK293FT cells revealed that the possible promoter activity regions (including positive and negative transcription factors binding sites simultaneously) presented between p Sp VIH-4 and p Sp VIH-6. In order to further identify the crucial transcription factors binding site in this region, the site-directed mutagenesis of Sox9/Oct4/Oct1 binding site of p Sp VIH-4 was created. The results demonstrated that the transcriptional activity of p Sp VIH-4△ decreased significantly (p SpVIH . Highlights cDNA, genomic DNA and the 5′-upstream regulatory sequences of SpVIH gene were obtained. SpVIH was only expressed in eyestalk. The expression level of SpVIH in eyestalk decreased gradually along with ovarian development. The possible promoter activity regions presented between pSpVIH-4 and pSpVIH-6. The Sox9/Oct4/Oct1 may be the essential positive transcription factor of SpVIH gene.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   Effects of thyroid hormone manipulation on pre-nuptial molt, luteinizing hormone and testicular growth in male white-crowned sparrows ( Zonotrichia leuchophrys gambelii )   SCI SCIE

    Pé (Department of Neurobiology, Physiology and Behavior, University of California, One Shields Avenue, Davis, CA 95616, USA ) , rez, Jonathan H. (The Roslin Institute, The Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG Scotland, UK ) , Meddle, Simone L. (Department of Neurobiology, Physiology and Behavior, University of California, One Shields Avenue, Davis, CA 95616, USA ) , Wingfield, John C. (Department of Neurobiology, Physiology and Behavior, University of California, One Shields Avenue, Davis, CA 95616, USA) , Ramenofsky, Marilyn
    General and comparative endocrinology v.255 ,pp. 12 - 18 , 2018 , 0016-6480 ,

    초록

    Highlights • Inhibition of thyroid hormone abolishes pre-nuptial molt and gonadal growth. • Exogenous T4 or T3 restores pre-nuptial molt. • Exogenous T4 but not T3 restores gonadal growth. • Methimazole inhibition of thyroid production increases luteinizing hormone. Most seasonal species rely on the annual change in day length as the primary cue to appropriately time major spring events such as pre-nuptial molt and breeding. Thyroid hormones are thought to be involved in the regulation of both of these spring life history stages. Here we investigated the effects of chemical inhibition of thyroid hormone production using methimazole, subsequently coupled with either triiodothyronine (T3) or thyroxine (T4) replacement, on the photostimulation of pre-nuptial molt and breeding in Gambel's white-crowned sparrows ( Zonotrichia leuchophrys gambelii ). Suppression of thyroid hormones completely prevented pre-nuptial molt, while both T3 and T4 treatment restored normal patterns of molt in thyroid hormone-suppressed birds. Testicular recrudescence was blocked by methimazole, and restored by T4 but not T3, in contrast to previous findings demonstrating central action of T3 in the photostimulation of breeding. Methimazole and replacement treatments elevated plasma luteinizing hormone levels compared to controls. These data are partially consistent with existing theories on the role of thyroid hormones in the photostimulation of breeding, while highlighting the possibility of additional feedback pathways. Thus we suggest that regulation of the hypothalamic pituitary gonad axis that controls breeding may be more complex than previously considered.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Age and socially related changes in fecal androgen metabolite concentrations in free-ranging male giraffes   SCI SCIE

    Wolf, T.E. (Endocrine Research Laboratory, Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa ) , Schaebs, F.S. (Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, Leipzig, Germany ) , Bennett, N.C. (Mammal Research Institute, Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0028, South Africa ) , Burroughs, R. (Centre of Veterinary Wildlife Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa ) , Ganswindt, A. (Endocrine Research Laboratory, Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa)
    General and comparative endocrinology v.255 ,pp. 19 - 25 , 2018 , 0016-6480 ,

    초록

    Abstract In many mammal species, androgen levels in males are elevated during periods of mating activity, often to facilitate aggressive behavior between males over access to fertile females. However, this pattern might be less obvious in species with a rather low male-male aggression rate, or in those that are not strictly seasonal breeders. A complex social structure, as well as additional social and environmental factors, might add more to the complexity. Here, we applied a non-invasive method to monitor fecal androgen metabolite (fAM) levels in free-ranging giraffe bulls over a period of months to examine longitudinal patterns of androgen metabolite concentrations in relation to observed male sexual behavior in different age classes. Giraffes are non-seasonal breeders, living in a fission–fusion social system and males show a roaming strategy to search for fertile females. Our results show that season has an impact on fAM levels in free-ranging giraffes, with respective steroid concentrations being higher in summer. In the presence of females, fAM levels of bulls are significantly higher compared to when found in all-male groups, with old adult bulls showing the highest fAM levels. In contrast, young adult bulls have overall slightly higher fAM levels compared to old adult bulls when residing in all male groups. Sexual behavior increases fAM levels only in old adult bulls. Highlights Season has an impact on fecal androgen metabolite (fAM) levels in free-ranging giraffes. In the presence of females, old adult bulls have higher fAM levels than younger adult bulls. In all male groups, young adult bulls have higher fAM levels than old adult bulls. Sexual activity increases fAM levels, but only in old adult bulls.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   The impact of age-class and social context on fecal glucocorticoid metabolite levels in free-ranging male giraffes   SCI SCIE

    Wolf, T.E. (Endocrine Research Laboratory, Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa ) , Bennett, N.C. (Mammal Research Institute, Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0028, South Africa ) , Burroughs, R. (Centre of Veterinary Wildlife Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa ) , Ganswindt, A. (Endocrine Research Laboratory, Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa)
    General and comparative endocrinology v.255 ,pp. 26 - 31 , 2018 , 0016-6480 ,

    초록

    Abstract One of the primary sources of perceived stress is the social environment of an animal and the interactions with conspecifics. An essential component of the response to a stressor is the activation of the hypothalamic–pituitary–adrenocortical axis, which results amongst others in a temporal increase in circulating glucocorticoid (GC) levels. Giraffes occur in a highly flexible fission-fusion social system and group compositions can change on a daily basis, with bulls establishing an age-related dominance hierarchy and showing a roaming strategy in the search for fertile females. The aim of this study was to non-invasively monitor the influence of different group compositions (mixed sex groups vs. all-male groups) on GC concentrations in free ranging giraffe bulls of different age classes. We collected fecal samples from free-ranging giraffe bulls for 12months in a South African Private Game Reserve to examine age- and social context-related patterns of fecal GC metabolite (fGCM) concentrations. We found that fGCM levels in giraffe bulls are age-class dependent, as well asassociated with changes in the social environment. Independently of the social setting, bulls of the youngest age class exhibited the highest fGCM levels compared to bulls of the other two older age-classes, with differences most pronounced when the bulls are associated in all-male groups. In contrast, an almost reversed picture appears when looking at the fGCM levels of sexually active individuals in mixed sex groups, where highest levels were found for the bulls in the oldest age-class, and the lowest for the bulls in the youngest age-class. The study stresses the importance to taking factors such asage-related status and social settings into account, when interpreting fGCM levels in free ranging giraffes. Highlights When in all male groups, sub-adult/juvenile bulls have the highest fGCM levels. In the presence of females, older sexually active adult bulls have the highest fGCM levels. Sexual activity leads to higher fGCM levels, but only in adult giraffe bulls.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   Seasonal ovarian immunolocalization of neuropeptide Y and its role in steriodogenesis in Asian catfish, Clarias batrachus   SCI SCIE

    Priyadarshini (Corresponding author.) , Lal, Bechan
    General and comparative endocrinology v.255 ,pp. 32 - 39 , 2018 , 0016-6480 ,

    초록

    Abstract The present study was undertaken to examine the cellular localization and potential steroidogenic role of neuropeptide Y (NPY) in the ovary of the freshwater catfish, Clarias batrachus . NPY-immunoreaction was observed in the follicular cells (granulosa and thecal cells) in the growing ovarian follicles, and the intensity of staining increased steadily from the initiation of follicular development until follicles were fully grown. Thereafter as follicles matured the stain intensity decreased. Positive correlations were found between NPY expression and the ovarian levels of 17β-estradiol, testosterone, and activities of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD) in the ovary. In vitro NPY treatment stimulated the production of the two steroids and the activities of two enzymes. This is the first report of NPY immunoreactivity at the cellular level in the fish ovary and implicates this orexigenic peptide in the modulation of ovarian steroidogenesis. Highlights NPY expresses in catfish granulosa and thecal cells of ovarian follicles. The expression of NPY increases in parallel with growth of ovarian follicles. NPY stimulates steroidogenesis in ovarian follicles.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Molecular mechanism of mercury-induced reproductive impairments in banded gourami, Trichogaster fasciata   SCI SCIE

    Guchhait, Rajkumar (Integrative Biology Research Unit, Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata 700073, India ) , Chatterjee, Ankit (Integrative Biology Research Unit, Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata 700073, India ) , Gupta, Shreyasi (Department of Zoology, University of Kalyani, Kalyani 741235, India ) , Debnath, Manashi (Integrative Biology Research Unit, Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata 700073, India ) , Mukherjee, Dilip (Department of Zoology, University of Kalyani, Kalyani 741235, India ) , Pramanick, Kousik (Integrative Biology Research Unit, Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata 700073, India)
    General and comparative endocrinology v.255 ,pp. 40 - 48 , 2018 , 0016-6480 ,

    초록

    Abstract Mercury is one of the key pollutants responsible for the degradation of natural aquatic ecosystems. Among the different forms of mercury that exist in the environment, mercuric chloride (HgCl 2 ) is the dominant pollutant for freshwater environments as it is used as an ingredient in antiseptics, disinfectants and preservatives, insecticides, batteries and in metallurgical and photographic operations. Pollutant may exert their action on organisms or populations by affecting their normal endocrine function as well as reproduction. Thus, the present study tried to understand the effect of mercuric chloride (HgCl 2 ) on reproductive function and to decipher the molecular mechanism of Hg-induced reproductive impairments of female Trichogaster fasciata . Both in vivo and in vitro experiments were performed by using ecologically relevant doses of HgCl 2 and the resulting effects on follicular development, steroidogenic potentiality, aromatase activity, aromatase gene expression and steroidogenic factor-1 (SF-1) expression pattern were analysed. In vivo exposure to HgCl 2 caused reproductive impairments as shown by the inhibitory role of HgCl 2 on follicular development, steroid biosynthesis and SF-1 activity. In vitro experiments revealed that aromatase activity, steroidogenesis, aromatase and SF-1 expression were blocked by HgCl 2 . The results obtained from this study contribute to understand the molecular mechanism of HgCl 2 -induced reproductive impairment of T. fasciata . Highlights Ecologically relevant doses of mercuric chloride acts as EDC in fish. HgCl 2 inhibits follicular development, steroidogenesis, aromatase activity and SF-1. Removal of mercury pollution regenerates the reproductive potentiality in T. fasciata . To conserve fish diversity, mitigation of pollution is the easiest way.

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  9. [해외논문]   Letrozole induced low estrogen levels affected the expressions of duodenal and renal calcium-processing gene in laying hens   SCI SCIE

    Li, Qiao (Corresponding author.) , Zhao, Xingkai , Wang, Shujie , Zhou, Zhenlei
    General and comparative endocrinology v.255 ,pp. 49 - 55 , 2018 , 0016-6480 ,

    초록

    Abstract Estrogen regulates the calcium homeostasis in hens, but the mechanisms involved are still unclear fully. In this study, we investigated whether letrozole (LZ) induced low estrogen levels affected the calcium absorption and transport in layers. In the duodenum, we observed a significant decrease of mRNA expressions of Calbindin-28k (CaBP-28k) and plasma membrane Ca 2+ -ATPase (PMCA 1b) while CaBP-28k protein expression was declined in birds with LZ treatment, and the mRNA levels of duodenal transient receptor potential vanilloid 6 (TRPV6) and Na + /Ca 2+ exchanger 1 (NCX1) were not affected. Interestingly, we observed the different changes in the kidney. The renal mRNA expressions of TRPV6 and NCX1 were unregulated while the PMCA1b was down-regulated in low estrogen layers, however, the CaBP-28k gene and protein expressions were no changed in the kidney. Furthermore, it showed that the duodenal estradiol receptor 2 (ESR2) transcripts rather than parathyroid hormone 1 receptor (PTH1R) and calcitonin receptor (CALCR) played key roles to down-regulate calcium transport in LZ-treated birds. In conclusion, CaBP-28k, PMCA 1b and ESR2 genes in the duodenum may be primary targets for estrogen regulation in order to control calcium homeostasis in hens. Highlights Estrogen has an important regulatory effect on calcium homeostasis. Low estrogen affected the calcium transport in laying hens. The expression of CaBP-28k protein responses to estrogen in a tissue-specific manner in hens. CaBP-28k, PMCA 1b and ESR2 genes in the duodenum may be primary targets for estrogen regulation in hens.

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  10. [해외논문]   The evolution and functional characterization of lined seahorse (Hippocampus erectus) CCKs involved in fasting and thermal stress response   SCI SCIE

    Zhang, Huixian (CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China ) , Qin, Geng (CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China ) , Sun, Jinhui (Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, PR China ) , Zhang, Bo (CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China ) , Lin, Qiang (CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China)
    General and comparative endocrinology v.255 ,pp. 56 - 63 , 2018 , 0016-6480 ,

    초록

    Abstract The peptide cholecystokinin (CCK) plays an important role in the regulation of vertebrate appetite and feeding behaviour. In the present study, the full-length cDNA and genomic DNA sequences of two CCK precursors were cloned and analysed in the Syngnathidae fish, the lined seahorse ( Hippocampus erectus ). Both CCK1 and CCK2 in the seahorse consist of four exons. The sequence of the octapeptide of seahorse CCK1 (DYMGWMDF) was the same as that of the chicken and human, while the octapeptide of seahorse CCK2 (DYEGWMDF) was unique among vertebrates. According to the phylogenetic analysis, two types of CCKs were produced by teleost-specific genome duplication (TGD). Both CCK1 and CCK2 were highly expressed in the brain, while detectable amounts of CCK1 mRNA in the brood pouch and CCK2 mRNA in the intestine were also found. Both CCK1 and CCK2 mRNA levels significantly increased during the transition from endogenous to exogenous nutrition. Additionally, fasting induced a significant increase in the CCK1 mRNA expression in the brain of juvenile seahorses but had no effect on CCK2 transcript levels. In addition, the CCK1 and CCK2 mRNA levels in the seahorse brain significantly increased after a high-temperature treatment. Thus, the mRNA expression of CCK had obvious tissue specificities and this preliminary study opens new avenues for further functional studies on the endocrine regulations of CCK in the transition from endogenous to exogenous nutrition, food intake regulation and metabolism in the seahorse. Highlights We report the molecular characterization of the CCK genes in Syngnathidae, a family special in male pregnancy. Both CCK1 and CCK2 mRNA levels significantly increased during the transition from endogenous to exogenous nutrition. Fasting induced a significant increase in the CCK1 mRNA expression in the brain of juvenile seahorses. Both CCK1 and CCK2 mRNA levels in the seahorse brain significantly increased after a high-temperature treatment.

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