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Biochemical and biophysical research communication...Biochemical and biophysical research communications 14건

  1. [해외논문]   Editorial Board  


    Biochemical and biophysical research communications v.502 no.4 ,pp. ii - ii , 2018 , 0006-291x ,

    초록

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  2. [해외논문]   USP10 regulates the stability of the EMT-transcription factor Slug/SNAI2  

    Ouchida, Amanda Tomie (Corresponding author.) , Kacal, Merve , Zheng, Adi , Ambroise, Gorbatchev , Zhang, Boxi , Norberg, Erik , Vakifahmetoglu-Norberg, Helin
    Biochemical and biophysical research communications v.502 no.4 ,pp. 429 - 434 , 2018 , 0006-291x ,

    초록

    Abstract Epithelial-to-mesenchymal transition (EMT) is a fundamental mechanism governing the switch of cells from an epithelial to a motile mesenchymal-like state. This transdifferentiation is regulated by key transcription factors, including Slug. The stability and function of Slug can be regulated by multiple mechanisms, including ubiquitin-mediated post-translational modifications. Here, by using a genome wide siRNA screen for human deubiquitinating enzymes (DUBs), we identified USP10 as a deubiquitinase for Slug in cancer cells. USP10 interacts with Slug and mediates its degradation by the proteasome. Importantly, USP10 is concomitantly highly expressed with Slug in cancer biopsies. Genetic knockdown of USP10 leads to suppressed Slug levels with a decreased expression of the mesenchymal marker Vimentin. Further, it reduces the migratory capacity of cancer cells. Reversely, overexpression of USP10 elevates the level of both Slug and Vimentin. Our study identifies USP10 as a regulator of the EMT-transcription factor Slug and cell migration. Highlights By undertaking a genome-wide siRNA screen, we have identified USP10 as regulator of Slug stability. We show that USP10 binds Slug and mediates its degradation by the proteasome. We provide detailed evidences that genetic depletion of USP10 causes beyond, a reduction of Slug, also suppress Vimetin levels (a hallmark mesenchymal marker) and migration. Reversely, expression of USP10 stabilizes and elevates Slug. Importantly, we show that USP10 expression correlates with Slug expression in primary tumor biopsies.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  3. [해외논문]   Methylmercury causes epigenetic suppression of the tyrosine hydroxylase gene in an in vitro neuronal differentiation model  

    Go, Suzuna (Corresponding author.) , Kurita, Hisaka , Matsumoto, Kana , Hatano, Manami , Inden, Masatoshi , Hozumi, Isao
    Biochemical and biophysical research communications v.502 no.4 ,pp. 435 - 441 , 2018 , 0006-291x ,

    초록

    Abstract Methylmercury (MeHg) is the causative substance of Minamata disease, which is associated with various neurological disorders such as sensory disturbance and ataxia. It has been suggested low-level dietary intake of MeHg from MeHg-containing fish during gestation adversely affects the fetus. In our study, we investigated the toxicological effects of MeHg exposure on neuronal differentiation focusing on epigenetics. We used human fetal brain-derived immortalized cells (LUHMES cells) as a human neuronal differentiation model. Cell viability, neuronal, and catecholamine markers in LUHMES cells were assessed after exposure to MeHg (0–1000 nM) for 6 days (from day 2 to day 8 of neuronal differentiation). Cell viability on day 8 was not affected by exposure to 1 nM MeHg for 6 days. mRNA levels of AADC , DBH , TUJ1 , and SYN1 also were unaffected by MeHg exposure. In contrast, levels of TH , the rate-limiting enzyme for dopamine synthesis, were significantly decreased after MeHg exposure. Acetylated histone H3, acetylated histone H3 lysine 9, and tri-methyl histone H3 lysine 9 levels at the TH gene promoter were not altered by MeHg exposure. However, tri-methylation of histone H3 lysine 27 levels, related to transcriptional repression, were significantly increased at the TH gene promotor after MeHg exposure. In summary, MeHg exposure during neuronal differentiation led to epigenetic changes that repressed TH gene expression. This study provides useful insights into the toxicological mechanisms underlying the effects of developmental MeHg exposure during neuronal differentiation. Highlights The low level of methylmercury induces inhibitory epigenetic changes in LUHMES cells. Tyrosine hydroxylase is decreased by methylmercury in neuronal differentiation model. HistoneH3 lysine27 tri-methylation is increased in promoter of tyrosine hydroxylase.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  4. [해외논문]   Generation of H1 PAX6WT/EGFP reporter cells to purify PAX6 positive neural stem/progenitor cells  

    Wu, Wei (Department of Pathophysiology, Key Lab for Shock and Microcirculation Research of Guangdong, Southern Medical University, Guangzhou, 510515, PR China ) , Liu, Juli (Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, PR China ) , Su, Zhenghui (Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, PR China ) , Li, Zhonghao (Department of Pathophysiology, Key Lab for Shock and Microcirculation Research of Guangdong, Southern Medical University, Guangzhou, 510515, PR China ) , Ma, Ning (Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regene) , Huang, Ke , Zhou, Tiancheng , Wang, Linli
    Biochemical and biophysical research communications v.502 no.4 ,pp. 442 - 449 , 2018 , 0006-291x ,

    초록

    Abstract Neural conversion from human pluripotent cells (hPSCs) is a potential therapy to neurological disease in the future. However, this is still limited by efficiency and stability of existed protocols used for neural induction from hPSCs. To overcome this obstacle, we developed a reporter system to screen PAX6 + neural progenitor/stem cells using transcription activator like effector nuclease (TALEN). We found that knock-in 2 A-EGFP cassette into PAX6 exon of human embryonic stem cells H1 with TALEN-based homology recombination could establish PAX6 WT/EGFP H1 reporter cell line fast and efficiently. This reporter cell line could differentiate into PAX6 and EGFP double positive neural progenitor/stem cells (NPCs/NSCs) after neural induction. Those PAX6 WT/EGFP NPCs could be purified, expanded and specified to post-mitotic neurons in vitro efficiently. With this reporter cell line, we also screened out 1 NPC-specific microRNA, hsa-miR-99a-5p, and 3 ESCs-enriched miRNAs, hsa-miR-302c-5p, hsa-miR-512–3p and hsa-miR-518 b. In conclusion, the TALEN-based neural stem cell screening system is safe and efficient and could help researcher to acquire adequate and pure neural progenitor cells for further application. Highlights We develop a talen-based gene targeting system to purify Pax6 + neural progenitor/stem cells by inserting reporter gene into endogenous pax6 gene locus of human pluripotent stem cells. The neural progenitor cell we purified possesses neural pluripotency and could be expanded and specified to post-mitotic neurons in vitro efficiently. With this reporter cell line, we also screened out 1 NPC-specific microRNA, hsa-miR-99a-5p, and 3 ESCs-enriched miRNAs, hsa-miR-302c-5p, hsa-miR-512–3p and hsa-miR-518 b.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Codon usage revisited: Lack of correlation between codon usage and the number of tRNA genes in enterobacteria  

    Rojas, Joaquí (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) , n (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) , Castillo, Gabriel (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) , Leiva, Lorenzo Eugenio (Department of Microbiology and The Center for RNA Biology, Ohio State University, Columbus, OH, 43210, USA ) , Elgamal, Sara (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile ) , Orellana, Omar (Department of Microbiology and The Center for RNA Biology, Ohio State University, Columbus, OH, 43210, USA ) , Ibba, Michael (Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile) , Katz, Assaf
    Biochemical and biophysical research communications v.502 no.4 ,pp. 450 - 455 , 2018 , 0006-291x ,

    초록

    Abstract It is widely believed that if a high number of genes are found for any tRNA in a rapidly replicating bacteria, then the cytoplasmic levels of that tRNA will be high and an open reading frame containing a higher frequency of the complementary codon will be translated faster. This idea is based on correlations between the number of tRNA genes, tRNA concentration and the frequency of codon usage observed in a limited number of strains as well as from the fact that artificially changing the number of tRNA genes alters translation efficiency and consequently the amount of properly folded protein synthesized. tRNA gene number may greatly vary in a genome due to duplications, deletions and lateral transfer which in turn would alter the levels and functionality of many proteins. Such changes are potentially deleterious for fitness and as a result it is expected that changes in tRNA gene numbers should be accompanied by a modification of the frequency of codon usage. In contrast to this model, when comparing the number of tRNA genes and the frequency of codon usage of several Salmonella enterica and Escherichia coli strains we found that changes in the number of tRNA genes are not correlated to changes in codon usage. Furthermore, these changes are not correlated with a change in the efficiency of codon translation. These results suggest that once a genome gains or loses tRNA genes, it responds by modulating the concentrations of tRNAs rather than modifying its frequency of codon usage. Highlights Average number of tRNA genes correlate with codon usage in highly expressed genes. Changes in the number of tRNA genes do not correlate to codon usage. Altered tRNA gene copy numbers do not correlate to changes of codon translation. Our data indicates that tRNA gene copies adapt to codon usage, but not the contrary.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   m6A demethylase FTO facilitates tumor progression in lung squamous cell carcinoma by regulating MZF1 expression  

    Liu, Jiqin (Corresponding author. Department of Clinical Experiment, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, 220 Chenglin Road, Tianjin, 300162, China.) , Ren, Dangli , Du, Zhenhua , Wang, Hekong , Zhang, Hua , Jin, Ying
    Biochemical and biophysical research communications v.502 no.4 ,pp. 456 - 464 , 2018 , 0006-291x ,

    초록

    Abstract N 6 -Methyladenosine (m 6 A) represents the most prevalent internal modification in mammalian mRNAs. Emerging evidences suggest that m 6 A modification is profoundly implicated in many biological processes, including cancer development. However, limited knowledge is available about the functional importance of m 6 A in lung cancer. In this study, by data mining The Cancer Genome Atlas (TCGA) database, we first identified fat mass- and obesity-associated protein (FTO) as a prognostic factor for lung squamous cell carcinoma (LUSC). Then we showed that FTO, but not other m 6 A modification genes including METTL3, METTL14 and ALKBH5, was the major dysregulated factor responsible for aberrant m 6 A modification in LUSC. Loss-of-function studies suggested that FTO knockdown effectively inhibited cell proliferation and invasion, while promoted cell apoptosis of L78 and NCI-H520 cells. Furthermore, overexpression of FTO, but not its mutant form, facilitated the malignant phenotypes of CHLH-1 cells. Mechanistically, FTO enhanced MZF1 expression by reducing m 6 A levels and mRNA stability in MZF1 mRNA transcript, leading to oncogenic functions. Taken together, our study demonstrates the functional importance of FTO in the tumor progression of LUSC and provides a potential therapeutic target for LUSC treatment. Highlights The prognostic value of m 6 A methyltransferase and demethylase in lung cancer. Differentially expressed FTO accounts for the aberrant m 6 A modification in LUSC. FTO acts as an oncogene in LUSC. MZF1 mediates FTO regulation in LUSC.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   Overexpressing circular RNA hsa_circ_0002052 impairs osteosarcoma progression via inhibiting Wnt/β-catenin pathway by regulating miR-1205/APC2 axis  

    Wu, Zhen (The Orthopaedic Department of Tongde Hospital of Zhejiang Province, Hangzhou, 310012, PR China ) , Shi, Wangping (Department of Pharmacy, Zhejiang Medical & Health Group Hangzhou Hospital, Hangzhou, 310022, PR China ) , Jiang, Chendi (Department of Orthopaedic Surgery, The Dingli Clinical Institute of Wenzhou Medical University, Wenzhou, 325000, PR China)
    Biochemical and biophysical research communications v.502 no.4 ,pp. 465 - 471 , 2018 , 0006-291x ,

    초록

    Abstract Circular RNAs (circRNAs) are a novel class of noncoding RNAs, whose importance in cancer has been gradually acknowledged. However, the functions of circRNAs in tumorigenesis have not been fully understood. In the present study, we identified a novel circRNA hsa_circ_0002052 significantly downregulated in osteosarcoma (OS) tissues and cell lines. Moreover, we found that hsa_circ_0002052 could act as a biomarker to indicate the prognosis of OS patients. Functionally, we showed that hsa_circ_0002052 overexpression significantly suppressed OS cell proliferation, migration and invasion while promoting apoptosis in vitro . Similarly, in vivo assay indicated that ectopic expression of hsa_circ_0002052 impaired OS cell growth. In terms of mechanism, we found that hsa_circ_0002052 inhibited miR-1205 while miR1205 targeted APC2, a negative regulator of Wnt/β-catenin signaling pathway. By releasing the inhibition of miR-1205 on APC2 expression, hsa_circ_0002052 suppressed the activation of Wnt/β-catenin signaling pathway, leading to attenuated OS progression. Taken together, our study for the first time revealed a suppressive circRNA hsa_circ_0002052 involved in OS progression. Our study suggested hsa_circ_0002052/miR-1205/APC2/Wnt/β-catenin axis might be a potential target for OS therapy.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  8. [해외논문]   Anti-viral immune response in the lung and thymus: Molecular characterization and expression analysis of immunoproteasome subunits LMP2, LMP7 and MECL-1 in pigs  

    Liu, Qiang (Corresponding author. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. ) , Hu, Wei (Corresponding author. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.) , Zhang, Yong-Li , Hu, Shou-Ping , Zhang, Zhuo , He, Xi-Jun , Cai, Xue-Hui
    Biochemical and biophysical research communications v.502 no.4 ,pp. 472 - 478 , 2018 , 0006-291x ,

    초록

    Abstract Both the lung and the thymus are vital target organ for pathogens including viruses. The immunoproteasome (i-proteasome) enhances antigen presentation for MHC class I molecules to activate CD8+T lymphocyte. These facilitate antiviral adaptive immune response. Our previous study found that, expression of i-proteasome subunits in porcine lung was altered during normal and inflammatory conditions. To date, the expression of i-proteasome subunits in porcine thymus to viruses has not been investigated. In the present study, LMP2, LMP7, and MECL-1 were cloned, identified and their sequences encoded predicted proteins of 216, 275, and 278 amino acids, respectively. Expression of LMP2, LMP7, and MECL-1, in the cytoplasm and nucleus, was markedly altered in the porcine reproductive and respiratory syndrome virus (PRRSV)-infected lung and thymus. And dendritic cells and epithelial cells readily expressed the i-proteasome subunit LMP2 in the thymus of PRRSV-infected pigs compared to that in mock-infected pigs. Additionally, the in vitro stimulation of a PAM cell line with PolyI:C for 12 and 24 h resulted in increased LMP2, LMP7, and MECL-1 expression. These results suggest a central role for these complexes in the activation of an antiviral immune response in pigs. A better understanding of the role of the i-proteasome in different cell types, tissues, and hosts could improve vaccine design and facilitate the development of effective treatment strategies for viral infections.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   Ectopic expression of the TAL effector AvrXa7 in Xanthomonas citri subsp. citri hinders citrus canker symptom formation by modulating transcriptional profile of citrus genes  

    Sun, Dongling (Corresponding author. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.) , Rou, Wei , Zhou, Yinghui , Zhuo, Tao , Fan, Xiaojing , Hu, Xun , Zou, Huasong
    Biochemical and biophysical research communications v.502 no.4 ,pp. 479 - 485 , 2018 , 0006-291x ,

    초록

    Abstract Xanthomonas citri subsp. citri ( Xcc ) is the causal agent of citrus canker, a serious bacterial disease that affects citrus trees worldwide. The ectopic expression of TAL effector AvrXa7 in Xcc suppressed canker development. The Xcc strain expressing avrXa7 induced a yellow symptom around the inoculation site. Transcriptome analysis revealed 315 differentially expressed genes, which were categorized into several functional groups. The more interesting genes were those involved in the biosynthesis of terpene and ethylene. In particular, the linoleate 13 S-lipoxygenase gene CsLOX2-1 was found to possess the AvrXa7 binding sequence in the promoter region. The recognition of AvrXa7 to the CsLOX2-1 promoter was subsequently confirmed by yeast one-hybrid and electrophoretic mobility shift experiments. This demonstrated that the TALE effector AvrXa7 promotes CsLOX2-1 expression by directly binding to the promoter sequence. Our findings contribute a valuable clue to identifying the potential genes that can be used to prevent citrus canker. Highlights Xanthomonas TAL effector AvrXa7 showed a suppression effect on citrus canker. The AvrXa7 modulated the transcription profile of citrus genes. The AvrXa7 targets CsLOX2-1 to promote citrus defense reaction. Graphical abstract [DISPLAY OMISSION]

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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    Fig. 1 이미지
  10. [해외논문]   miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse  

    Wang, Xiaodan (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Zhang, Peng (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Li, Leijie (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Che, Dongxue (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Li, Tongtong (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Li, Hao (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Li, Qun (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Jia, Haiyang (College of Computer Science and Technology, Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, Jilin University, Changchun, 130012, China ) , Tao, Shiheng (College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China ) , Hua, Jinlian (College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling,) , Zeng, Wenxian , Liao, Mingzhi
    Biochemical and biophysical research communications v.502 no.4 ,pp. 486 - 492 , 2018 , 0006-291x ,

    초록

    Abstract Spermatogenesis has a close relationship with male infertility. MicroRNAs (miRNAs) play crucial roles in their regulation of target genes during spermatogenesis. A huge dataset of high-throughput sequencing all over the world provides the basis to dig the cryptic molecular mechanism. But how to take advantage of the big data and unearth the miRNA regulation is still a challenging problem. Here we integrated transcriptome of spermatogenesis and found miRNA regulate spermatogenesis through miRNA editing. We then compared different species and found that the distributions of miRNA editing site number and editing types among different cell types during spermatogenesis are conservative. Interesting, we further found that nearly half of the editing events occurred in the seed region in both mouse and pig. Finally, we foundmiR-34c, which is edited frequently at all stages during spermatogenesis, regulates its target genes through the RNA structure changing and shows dysfunction when it is edited. Summary, we depicted the overall profile of miRNA editing during spermatogenesis in mouse and pig and reveal miR-34c may play its roles through miRNA editing. Highlights We integrated transcriptome of spermatogenesis and found miRNA regulate spermatogenesis through miRNA editing. We compared with different species and found that the distribution of miRNA editing during spermatogenesis is conservative. We identified miR-34c, which is edited frequently at all stages during spermatogenesis, regulates its target genes through the RNA structure changing and shows dysfunction when it is edited.

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