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Analytical biochemistry 12건

  1. [해외논문]   Editorial Board   SCI SCIE


    Analytical biochemistry v.538 ,pp. ii - iii , 2017 , 0003-2697 ,

    초록

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

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  2. [해외논문]   Method to minimize ozone effect on Cy5 fluorescent intensity in DNA microarrays   SCI SCIE

    Kim, Youngjun (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , Seo, Hyun Hee (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , Jeong, Mi Seon (Laboratory of Research and Development for Genomics, Cheil General Hospital, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , Lee, Ki Heon (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , Lee, In Ho (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , So, Kyeong A. (Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, 17, Seoae ro 1 Gil, Jung gu, Seoul, Republic of Korea ) , Kim, Mi Kyung (Department of Obstetrics and) , Lee, Yoo-Kyung , Kim, Seon Ah , Kim, Tae Jin
    Analytical biochemistry v.538 ,pp. 1 - 4 , 2017 , 0003-2697 ,

    초록

    Abstract Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level. Consistent results in microarray analysis were obtained using Cy5 dye under atmospheric ozone.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [해외논문]   Colorimetric detection of D-dimer in a paper-based immunodetection device   SCI SCIE

    Ruivo, Só (Corresponding author.) , nia , Azevedo, Ana M. , Prazeres, Duarte M.F.
    Analytical biochemistry v.538 ,pp. 5 - 12 , 2017 , 0003-2697 ,

    초록

    Abstract A microfluidic paper-based analytical device (μPADs) immunoassay for detection of the blood native biomarker D-dimer is reported. The μPAD is created by wax printing on a single piece of chromatographic paper and combined with an anti-D-dimer capture antibody and conjugates of anti-D-dimer antibody with 40 nm gold nanoparticles. The presence of D-dimer in buffer/simulated plasma samples is successfully reported for concentrations as low as 15 ng D-dimer/mL. Linearity between signal intensity and D-dimer concentration is observed up to 100 ng/mL. Using an appropriate dilution, the test could be used to yield positive results only for those samples with a D-dimer concentration above the clinically relevant threshold range of 250–500 ng/mL. Finally, the merits and pitfalls of using μPADs as compared to lateral flow devices in immunoassays are discussed.

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    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [해외논문]   Ultrasensitive and rapid immuno-detection of human IgE anti-therapeutic horse sera using an electrochemical immunosensor   SCI SCIE

    Prado, Isis C. (FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil ) , Souza, André (FIOCRUZ, Oswaldo Cruz Institute, Laboratory of Experimental and Computational Biochemistry of Pharmaceuticals, Rio de Janeiro, RJ, Brazil ) , L.A. (FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil ) , Provance-Jr, David W. (Federal Fluminense University, Chemistry Institute, Analytical Chemistry Department, Niterói, Rio de Janeiro, Brazil ) , Cassella, Ricardo J. (FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil) , De-Simone, Salvatore G.
    Analytical biochemistry v.538 ,pp. 13 - 19 , 2017 , 0003-2697 ,

    초록

    Abstract Antivenom allergy disease mediated by patient IgE is an important public health care concern. To improve detection of hypersensitive individuals prior to passive antibody therapy, an amperometric immunosensor was developed to detect reactive human IgE. Whole horse IgG3 (hoIgG3) was immobilized onto the surface of carbon or gold screen-printed electrodes through a cross-linking solution of glutaraldehyde on a chitosan film. Sera from persons with a known allergic response to hoIgG3 or non-allergic individuals was applied to the sensor. Bound human IgE (humIgE) was detected by an anti-humIgE antibody through a quantitative amperometric determination by tracking via the electrochemical reduction of the quinone generated from the hydroquinone with the application of a potential of 25 mV. The optimal immunosensor configuration detected reactive humIgE at a dilution of 1:1800 of the human sera that represent a detection limit of 0.5 pg/mL. Stability testing demonstrated that through 20 cycles of a scan, the specificity and performance remained robust. The new immunosensor successfully detected humIgE antibodies reactive against hoIgG3, which could allow the diagnosis of potential allergenic patients needing therapeutic antivenom preparations from a horse.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [해외논문]   Ultrasensitive microRNA-21 detection based on DNA hybridization chain reaction and SYBR Green dye   SCI SCIE

    Li, Zhi (College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China ) , Li, Bingchen (College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China ) , Zhou, Yunlei (College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China ) , Yin, Huanshun (College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China ) , Wang, Jun (College of Resources and Environment, Shandong Agricultural University, 271018, Taian, Shandong, PR China ) , Ai, Shiyun (College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China)
    Analytical biochemistry v.538 ,pp. 20 - 25 , 2017 , 0003-2697 ,

    초록

    Abstract It is extremely important for quantifying trace microRNAs in the biomedical applications. In this study, an ultrasensitive, rapid and efficient label-free fluorescence method was proposed and applied for detecting microRNA-21 in serum of gastric cancer patients based on DNA hybridization chain reaction (HCR). DNA H1 and DNA H2 were designed and used as hairpin probes, the HCR was proceeded in the presence of target microRNAs. Amounts of SYBR Green І dyes were used as signal molecules to intercalate long DNA concatemers from HCR, which guaranteed the model of label-free fluorescence and strong fluorescence density. The detection method showed a wide linear region from 1 fM to 10 5 fM, and the limit of detection was 0.2554 fM (at S/N = 3) for microRNAs. The results showed that this method had an excellent specificity and reproducibility. Furthermore, the label-free fluorescence strategy exhibited a sensitive response to microRNA-21 in real serum samples of gastric cancer patients and the results obtained were in accordance with reference method (R 2 = 0.994). Overall, the proposed strategy could be satisfactory for rapid, ultrasensitive and efficient detection of microRNA-21, and held great potentials in clinic diagnosis of gastric cancer. Graphical abstract [DISPLAY OMISSION]

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    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [해외논문]   Fluorescence and magnetic nanocomposite Fe3O4@SiO2@Au MNPs as peroxidase mimetics for glucose detection   SCI SCIE

    Luo, Shajie (College of Science, Sichuan Agricultural University, Ya'an 625014, China ) , Liu, Yaqin (College of Science, Sichuan Agricultural University, Ya'an 625014, China ) , Rao, Hanbing (College of Science, Sichuan Agricultural University, Ya'an 625014, China ) , Wang, Yanying (College of Science, Sichuan Agricultural University, Ya'an 625014, China ) , Wang, Xianxiang (College of Science, Sichuan Agricultural University, Ya'an 625014, China)
    Analytical biochemistry v.538 ,pp. 26 - 33 , 2017 , 0003-2697 ,

    초록

    Abstract In this paper, multifunction nanoparticles (MNPs), Fe 3 O 4 @SiO 2 @Au MNPs, with properties of superparamagnetism, fluorescence and peroxidase-like catalytic activity were synthesized in the aqueous phase. The synthesized composites were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier translation infrared spectrum (FT-IR) and fluorometer. The results show that the multifunctional nanomaterials have good magnetic and fluorescence properties. Then, the mimetic properties of this material were investigated. The as-synthesized Fe 3 O 4 @SiO 2 @Au MNPs exhibited the best catalytic activity for peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) at the reaction temperature of 70 °C and pH of 3. Compared with free Fe 3 O 4 MNPs and BSA-Au nanoclusters (NCs), the composites have better catalytic activity at higher temperature and lower pH, indicating that Fe 3 O 4 @SiO 2 @Au MNPs can work in more severe environment. In practical application, we have successfully established the colorimetric method for the detection of H 2 O 2 and glucose with the detection range of 1 × 10 −6 ∼ 4 × 10 −5 M and 5 × 10 −6 ∼ 3.5 × 10 −4 M, and the detection limit of 6 × 10 −7 M and 3.5 × 10 −6 M, respectively. The method was also successfully applied in the detection of real samples. Furthermore, since the fluorescence of Fe 3 O 4 @SiO 2 @Au MNPs was quenched by H 2 O 2 , a method for the visual detection of glucose was established. Graphical abstract [DISPLAY OMISSION]

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   Gas chromatography tandem mass spectrometry offers advantages for urinary steroids analysis   SCI SCIE

    Homer, Natalie (Edinburgh Clinical Research Facility Mass Spectrometry Core, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK ) , Kothiya, Sanjay (University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK ) , Rutter, Alison (University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK ) , Walker, Brian R. (University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK ) , Andrew, Ruth (University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK)
    Analytical biochemistry v.538 ,pp. 34 - 37 , 2017 , 0003-2697 ,

    초록

    Abstract Gas chromatography mass spectrometry has been the lynchpin of clinical assessment of steroid profiles for ∼3 decades. The improvements in assay performance offered by tandem mass spectrometry were assessed. Across the spectrum of glucocorticoid and androgen analytes tested, limits of detection and quantitation were ∼20 fold lower with triple than single quadrupole systems, but the more noticeable improvement was that signal to noise was substantially improved and the linear range wider. These benefits allowed more reliable and concomitant measurement of steroids with substantially different abundances and in smaller volumes of urine.

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    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [해외논문]   Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum   SCI SCIE

    Giustarini, Daniela (Department of Medicine, Surgery and Neurosciences, University of Siena, Via A. Moro 2, I-53100 Siena, Italy ) , Galvagni, Federico (Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via A. Moro 2, I-53100 Siena, Italy ) , Colombo, Graziano (Department of Biosciences, Università) , Dalle-Donne, Isabella (degli Studi di Milano, Via Celoria 26, I-20133 Milan, Italy ) , Milzani, Aldo (Department of Biosciences, Università) , Aloisi, Anna Maria (degli Studi di Milano, Via Celoria 26, I-20133 Milan, Italy ) , Rossi, Ranieri (Department of Biosciences, Università)
    Analytical biochemistry v.538 ,pp. 38 - 41 , 2017 , 0003-2697 ,

    초록

    Abstract We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [해외논문]   Menadione-mediated WST1 reduction assay for the determination of metabolic activity of cultured neural cells   SCI SCIE

    Stapelfeldt, Karsten (Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, Bremen, Germany ) , Ehrke, Eric (Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, Bremen, Germany ) , Steinmeier, Johann (Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, Bremen, Germany ) , Rastedt, Wiebke (Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, Bremen, Germany ) , Dringen, Ralf (Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, Bremen, Germany)
    Analytical biochemistry v.538 ,pp. 42 - 52 , 2017 , 0003-2697 ,

    초록

    Abstract Cellular reduction of tetrazolium salts to their respective formazans is frequently used to determine the metabolic activity of cultured cells as an indicator of cell viability. For membrane-impermeable tetrazolium salts such as WST1 the application of a membrane-permeable electron cycler is usually required to mediate the transfer of intracellular electrons for extracellular WST1 reduction. Here we demonstrate that in addition to the commonly used electron cycler M-PMS, menadione can also serve as an efficient electron cycler for extracellular WST1 reduction in cultured neural cells. The increase in formazan absorbance in glial cell cultures for the WST1 reduction by menadione involves enzymatic menadione reduction and was twice that recorded for the cytosolic enzyme-independent WST1 reduction in the presence of M-PMS. The optimized WST1 reduction assay allowed within 30 min of incubation a highly reliable detection of compromised cell metabolism caused by 3-bromopyruvate and impaired membrane integrity caused by Triton X-100, with a sensitivity as good as that of spectrophotometric assays which determine cellular MTT reduction or lactate dehydrogenase release. The short incubation period of 30 min and the observed good sensitivity make this optimized menadione-mediated WST1 reduction assay a quick and reliable alternative to other viability and toxicity assays.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [해외논문]   Glycan profiling of proteins using lectin binding by Surface Plasmon Resonance   SCI SCIE

    Wang, Wei (Corresponding author. One Amgen Center Drive, MS 29-1-B, Thousand Oaks, CA 91320, USA. ) , Soriano, Brian (Corresponding author. One Amgen Center Drive, MS 29-1-B, Thousand Oaks, CA 91320, USA.) , Chen, Qing
    Analytical biochemistry v.538 ,pp. 53 - 63 , 2017 , 0003-2697 ,

    초록

    Abstract Glycan profiling of proteins was studied through their lectin binding activity by Surface Plasmon Resonance (SPR). To validate the method, we monitored specific lectin binding with sequential removal of sugar moieties from human transferrin using specific glycosidases. The results clearly indicated that glycans on the protein can be identified by their selective binding activity to various lectins. Using this method, we characterized Fc glycosylation profiles of therapeutic peptibodies and antibodies expressed in mammalian cells (CHO and HEK 293 6E cells), with E. coli expressed proteins as the negative controls. We observed that antibodies expressed in CHO cells did not contain any sialic acid, while antibodies expressed in 293 6E cells contained sialic acid. CHO cell expressed antibodies were also more heavily fucosylated than the ones expressed by 293 6E cells. We further applied this method to measure the fucose composition of glycan engineered mouse antibodies, as well as to determine mannose composition of human antibody variants with depletion or enrichment of high mannose. The glycan profiles generated using this method were comparable to results from 2-AB labeled glycan analysis of normal-phase separated glycans, and Fc gamma receptor binding activity of the glycan engineered antibodies were consistent with their glycan profiles. Hence, we demonstrated that SPR lectin binding analysis can be a quick alternative method to profile protein glycosylation.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

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