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Biomedical chromatography : BMC 27건

  1. [해외논문]   Issue information  


    Biomedical chromatography : BMC v.32 no.8 ,pp. NA , 2018 , 0269-3879 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  2. [해외논문]   Simultaneous determination of 20(S)‐protopanaxadiol and its three metabolites in rat plasma by LC–MS/MS: application to their pharmacokinetic studies  

    Li, Jin‐ (Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China) , Qi (School of Medicine, University of Electronic Science and Technology of China, Chengdu, China) , Wang, Jia‐ (School of Medicine, University of Electronic Science and Technology of China, Chengdu, China) , Feng (School of Medicine, University of Electronic Science and Technology of China, Chengdu, China) , Li, Jie (School of Medicine, University of Electronic Science and Technology of China, Chengdu, China) , Zhang, Shu‐ (Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China) , han (Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China) , He, Dan , Tong, Rong‐ , Sheng , She, Shu‐ , Ya
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4252 , 2018 , 0269-3879 ,

    초록

    Abstract The aim of this study was to develop an LC–MS/MS method for simultaneous determination of 20( S ) protopanaxadiol (PPD) and its three metabolites, PPD‐glucuronide (M1), (20 S ,24 S )‐epoxy‐dammarane‐3,12,25‐triol (M2) and (20 S ,24 R )‐epoxydammarane‐3,12,25‐triol (M3), in rat plasma. Precipitation with acetonitrile was employed for sample preparation and chromatographic separations were achieved on a C 18 column. The sample was detected using triple quadrupole tandem mass spectrometer with selected reaction monitoring mode. The monitored precursor‐to‐product ion transitions were m/z 459.4 → 375.3 for PPD, m/z 635.4 → 113.0 for M1, m/z 477.4 → 441.4 for M2 and M3 and m/z 475.4 → 391.3 for IS. The developed assay was validated according to the guidelines of the US Food and Drug Administration. The calibration curves showed good linearity over the tested concentration ranges ( r > 0.9993), with the LLOQ being 1 ng/mL for all analytes. The intra‐ and inter‐day precisions (RSD) were 80% and no obvious matrix effect was detected. The analytes were stable in rat plasma with the RE ranging from −12.34 to 9.77%. The validated assay has been successfully applied to the pharmacokinetic study of PPD as well as its metabolites in rat plasma. According to the pharmacokinetic parameters, the in vivo exposures of M1, M2 and M3 were 11.91, 47.95 and 22.62% of that of PPD, respectively.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   A validated LC–MS/MS method for the simultaneous determination of thalidomide and its two metabolites in human plasma: Application to a pharmacokinetic assay  

    Jiang, Fulin (Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, North Campus, Sun Yat‐Sen University, Guangzhou, China) , Peng, Xiang (The Sixth Affiliated Hospital of Sun Yat‐sen Univesity, Guangdong Gastrointestinal Hospital, Guangzhou, China) , Cai, Dake (Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine, Guangzhou, Guangdong, People's Republic of China) , Wen, Dingsheng (Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, North Campus, Sun Yat‐Sen University, Guangzhou, China) , Liu, Yao (Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, North Campus, Sun Yat‐Sen University, Guangzhou, China) , Zhi, Min (The Sixth Affiliated Hospital of Sun Yat‐sen Univesity, Guangdong Gastrointestinal Hos) , Chen, Jiangying , Hu, Pinjin , Wang, Xueding , Gao, Yujie , Huang, Min , Gao, Xiang , Zhong, Guoping
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4240 , 2018 , 0269-3879 ,

    초록

    Abstract An accurate and sensitive LC–MS/MS method for determining thalidomide, 5‐hydroxy thalidomide and 5′‐hydroxy thalidomide in human plasma was developed and validated using umbelliferone as an internal standard. The analytes were extracted from plasma (100 μL) by liquid–liquid extraction with ethyl acetate and then separated on a BETASIL C 18 column (4.6 × 150 mm, 5 μm) with mobile phase composed of methanol–water containing 0.1% formic acid (70:30, v /v) in isocratic mode at a flow rate of 0.5 mL/min. The detection was performed using an API triple quadrupole mass spectrometer in atmospheric pressure chemical ionization mode. The precursor‐to‐product ion transitions m / z 259.1 → 186.1 for thalidomide, m / z 273.2 → 161.3 for 5‐hydroxy thalidomide, m / z 273.2 → 146.1 for 5′‐hydroxy thalidomide and m / z 163.1 → 107.1 for umbelliferone (internal standard, IS) were used for quantification. The calibration curves were obtained in the concentrations of 10.0–2000.0 ng/mL for thalidomide, 0.2–50.0 ng/mL for 5‐hydroxy thalidomide and 1.0–200.0 ng/mL for 5′‐hydroxy thalidomide. The method was validated with respect to linear, within‐ and between‐batch precision and accuracy, extraction recovery, matrix effect and stability. Then it was successfully applied to estimate the concentration of thalidomide, 5‐hydroxy thalidomide and 5′‐hydroxy thalidomide in plasma samples collected from Crohn's disease patients after a single oral administration of thalidomide 100 mg.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   A simple and selective LC‐MS/MS method for quantification of ikarisoside A in rat plasma and its application to a pharmacokinetic study  

    Cong, Yue (Department of Ocular Fundus Disease, the Second Hospital of Jilin University, Changchun, China) , Wang, Rui (Department of Hematology, the First Hospital of Jilin University, Changchun, China) , Zhang, Chen (Department of Hematology, the First Hospital of Jilin University, Changchun, China) , Lin, Hai (Department of Hematology, the First Hospital of Jilin University, Changchun, China)
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4245 , 2018 , 0269-3879 ,

    초록

    Abstract Ikarisoside A is a natural flavonoid isolated from Epimedium plants. To further evaluate its medicinal potential, a sensitive and robust LC–MS/MS method was developed and validated for the assay of ikarisoside A in rat plasma. Orientin was used as an internal standard. The electrospray ionization was operated in its negative ion mode while ikarisoside A and IS were measured by selected reaction monitoring using precursor‐to‐product ion transitions of m/z 499.1 → 353.0 and m/z 446.9 → 327.6, respectively. This LC–MS/MS method had good sensitivity (LLOQ = 1.5 ng/mL), accuracy (both intra‐ and inter‐day RE ≤ ±11.9%) and precision (both intra‐ and inter‐day RSD ≤8.5%). The pharmacokinetics of ikarisoside A was subsequently profiled in Sprague–Dawley rats. Following oral administration (35 mg/kg), ikarisoside A reached maximum plasma concentration ( C max , 207.6 ± 96.7 ng/mL) attained at 1.10 ± 0.42 h. Following oral administration, the clearance and terminal half‐life were 42.9 ± 26.5 L/h/kg and 3.15 ± 0.80 h by oral route, respectively.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Development of new extraction method based on liquid–liquid–liquid extraction followed by dispersive liquid–liquid microextraction for extraction of three tricyclic antidepressants in plasma samples  

    Farajzadeh, Mir Ali (Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran) , Abbaspour, Maryam (Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran)
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4251 , 2018 , 0269-3879 ,

    초록

    Abstract In the present study, a new extraction method based on a three–phase system, liquid–liquid–liquid extraction, followed by dispersive liquid–liquid microextraction has been developed and validated for the extraction and preconcentration of three commonly prescribed tricyclic antidepressant drugs – amitriptyline, imipramine, and clomipramine – in human plasma prior to their analysis by gas chromatography–flame ionization detection. The three phases were an aqueous phase (plasma), acetonitrile and n –hexane. The extraction mechanism was based on the different affinities of components of the biological sample (lipids, fatty acids, pharmaceuticals, inorganic ions, etc.) toward each of the phases. This provided high selectivity toward the analytes since most interferences were transferred into n –hexane. In this procedure, a homogeneous solution of the aqueous phase (plasma) and acetonitrile (water–soluble extraction solvent) was broken by adding sodium sulfate (as a phase separating agent) and the analytes were extracted into the fine droplets of the formed acetonitrile. Next, acetonitrile phase was mixed with 1,2–dibromoethane (as a preconcentration solvent at microliter level) and then the microextraction procedure mentioned above was performed for further enrichment of the analytes. Under the optimum extraction conditions, limits of detection and lower limits of quantification for the analytes were obtained in the ranges of 0.001–0.003 and 0.003–0.010 μg mL −1 , respectively. The obtained extraction recoveries were in the range of 79–98%. Intra– and inter–day precisions were

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   Fast and sensitive HPLC/UV method for cefazolin quantification in plasma and subcutaneous tissue microdialysate of humans and rodents applied to pharmacokinetic studies in obese individuals  

    Palma, Eduardo Celia (Pharmacokinetics and PK/PD Modeling Laboratory, Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil) , Laureano, Joã (Pharmacokinetics and PK/PD Modeling Laboratory, Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil) , o Victor (Pharmacokinetics and PK/PD Modeling Laboratory, Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil) , de Araú (Center for Obese Class III Care, Nossa Senhora Conceição Hospital (HNSC), Porto Alegre, RS, Brazil) , jo, Bibiana Verlindo (Center for Obese Class III Care, Nossa Senhora Conceição Hospital (HNSC), Porto Alegre, RS, Brazil) , Meinhardt, Nelson Guardiola (Pharmacokinetics and PK/PD Modeling Laborat) , Stein, Airton Tetelbom , Dalla Costa, Teresa
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4254 , 2018 , 0269-3879 ,

    초록

    Abstract Antimicrobial prophylactic dosing of morbidly obese patients may differ from normal weighted individuals owing to alterations in drug tissue distribution. Drug subcutaneous tissue distribution can be investigated by microdialysis patients and animals. The need for cefazolin prophylactic dose adjustment in obese patients remains under discussion. The paper describes the validation of an HPLC‐UV method for cefazolin quantification in plasma and microdialysate samples from clinical and pre‐clinical studies. A C 18 column with an isocratic mobile phase was used for drug separation, with detection at 272 nm. Total and unbound cefazolin lower limit of quantitation was 5 μg/mL in human plasma, 2 μg/mL in rat plasma, and 0.5 and 0.025 μg/mL in human and rat microdialysate samples, respectively. The maximum intra‐ and inter‐day imprecisions were 10.7 and 8.1%, respectively. The inaccuracy was

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   A robust LC–MS/MS method for the simultaneous determination of docetaxel and voriconazole in rat plasma and its application to pharmacokinetic studies  

    Pei, Xuexue (State Key Laboratory of Natural Medicines, Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China) , Li, Peijin (State Key Laboratory of Natural Medicines, Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China) , Jiang, Wenjiao (State Key Laboratory of Natural Medicines, Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China) , Sun, Yilin (State Key Laboratory of Natural Medicines, Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China) , Lu, Li (State Key Laboratory of Natural Medicines, Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China) , Ding, Fei , Wang, Guangji , Hao, Kun
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4246 , 2018 , 0269-3879 ,

    초록

    Abstract Opportunistic fungal infections are common in immunocompromised cancer patients, especially patients undergoing chemotherapy. Because antitumor agents are possible to combine with antifungal agents in clinical, it is necessary to study drug–drug interaction between antitumor agents and antifungal agents. The aim of the study was to explore a method for the simultaneous determination of voriconazole and docetaxel in plasma and investigate pharmacokinetic interaction of voriconazole and docetaxel in rats. A precise and reliable method using liquid chromatography tandem mass spectrometry (LC–MS/MS) was established for the simultaneous measure of docetaxel and voriconazole in rat plasma after liquid–liquid extraction with ethyl acetate. The method was fully validated and successfully applied to a pharmacokinetic interaction study of docetaxel and voriconazole in rats after single or combined administration. We found that the AUC of each drug after coadministration increased compared with that after the single administration, which might be caused by interaction at the absorption stage or the competitive inhibition on the metabolic enzymes. This established method can be utilized to study the detailed mechanism of the drug–drug interaction and guide rational drug use in the clinic.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Development and validation of a HPLC‐UV assay for quantification of levetiracetam concentrations in critically ill patients undergoing continuous renal replacement therapy  

    Kalaria, Shamir N. (Center for Translational Medicine, University of Maryland School of Pharmacy, Baltimore, Maryland, USA) , Dahmane, Elyes (Center for Translational Medicine, University of Maryland School of Pharmacy, Baltimore, Maryland, USA) , Armahizer, Michael (Department of Pharmacy, University of Maryland Medical Center, Baltimore, Maryland, USA) , McCarthy, Paul (Department of Nephrology, University of Maryland Medical Center, Baltimore, Maryland, USA) , Gopalakrishnan, Mathangi (Center for Translational Medicine, University of Maryland School of Pharmacy, Baltimore, Maryland, USA)
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4257 , 2018 , 0269-3879 ,

    초록

    Abstract Limited clinical data exists on the effects of continuous renal replacement therapy (CRRT) on drug pharmacokinetics. A high‐performance liquid chromatography with ultraviolet detection method was developed and validated to determine levetiracetam concentrations in human plasma and CRRT effluent samples. Five hundred microliters of human plasma and 250 μL effluent samples were used to quantify levetiracetam. Plasma samples were purified by protein precipitation, evaporated under nitrogen gas at room temperature and reconstituted in 50 m m potassium dihydrogen phosphate buffer (pH of 4.5). Reverse‐phase chromatographic separation was achieved within 20 min using a mobile phase eluting gradient of 50 m m potassium dihydrogen phosphate and acetonitrile. UV detection was set at 195 nm. The calibration curve was found to be linear over the range of 2–80μg/mL. Inter‐ and intra‐day precisions were

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   Development of a novel ion‐pairing HPLC‐FL method for the separation and quantification of hydroxychloroquine and its metabolites in whole blood  

    Charlier, Bruno (Postgraduate School in Hospital Pharmacy, University of Salerno, Fisciano (Salerno), Italy) , Pingeon, Marine (Department of Medicine, Surgery and Dentistry, University of Salerno, Baronissi (Salerno), Italy) , Dal Piaz, Fabrizio (Department of Medicine, Surgery and Dentistry, University of Salerno, Baronissi (Salerno), Italy) , Conti, Valeria (Department of Medicine, Surgery and Dentistry, University of Salerno, Baronissi (Salerno), Italy) , Valentini, Gabriele (Department of Clinical and Experimental Medicine, University of Campania 'Luigi Vanvitelli', Rheumatology Section, Naples, Italy) , Filippelli, Amelia (Department of Medicine, Surgery and Dentistry, University of Salerno, Baronissi (Salerno), Italy) , Izzo, Viviana (Department of Medicine, Surgery and Dentistry, University of Salerno, Baronissi (Salerno), Italy)
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4258 , 2018 , 0269-3879 ,

    초록

    Abstract Hydroxychloroquine (HCQ) is an old antimalarial drug that has proven to be a safe and effective treatment for systemic lupus erythematosus (SLE) and other autoimmune diseases. Since hematic concentration of HCQ is closely related to the therapeutic response, monitoring the levels of the drug and its metabolites in the blood of HCQ‐treated patients helps the clinician in the evaluation of partial or complete unresponsiveness to treatment. We developed and validated a novel ion‐pairing HPLC‐FL method for the simultaneous dosage of HCQ, and its major metabolites desethylhydroxychloroquine, desethylchloroquine and bisdesethylchloroquine, after extraction from whole blood. This methodological approach was used for the analysis of real samples obtained from patients affected by SLE and undergoing HCQ treatment. The same samples were also analyzed using a previously validated LC/MS/MS method and data obtained with the two approaches were in substantial agreement with each other. Results presented in this work indicate that this approach can be successfully used to monitor the level of HCQ and its metabolites in the blood of various categories of patients (i.e. low and high responders, or those not adhering to the therapy). Comparison of HPLC‐FL and LC/MS/MS data confirmed the efficacy of the proposed method for routine clinical analyses.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [해외논문]   Simultaneous determination of twelve polar pteridines including dihydro‐ and tetrahydropteridine in human urine by hydrophilic interaction liquid chromatography with tandem mass spectrometry  

    Xiong, Xin (Department of Pharmacy, Peking University Third Hospital, Beijing, China) , Zhang, Yuanyuan (Department of Pharmacy, Peking University Third Hospital, Beijing, China) , Zhang, Wenjing (Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China)
    Biomedical chromatography : BMC v.32 no.8 ,pp. e4244 , 2018 , 0269-3879 ,

    초록

    Abstract Pteridines and their derivatives are important cofactors in the process of cell metabolism, and the level of urinary excretion of these compounds is considered as an important clinical criterion. In this work, a new separation method involving hydrophilic interaction chromatography (HILIC) with tandem mass spectrometric detection has been developed for the simultaneous analysis of 12 pteridines including oxidized, di‐ and tetrahydroforms, namely neopterin, 7,8‐dihydroneopterin, biopterin, 7,8‐dihydrobiopterin, 5,6,7,8‐tetrahydrobiopterin, dimethylpterin, dimethyltetrahydropterin, pterin, isoxanthopterin, xanthopterin, sepiapterin and pterin‐6‐carboxylic acid, in human urine without oxidative pretreatments. The stabilizing agent (dithiothreitol) at various concentrations and the stability of oxidized, di‐ and tetrahydroforms during the sample's short‐term storage and processing and of the extracts were tested. In the developed method, 12 pteridines were chromatographically separated on an ZIC‐HILIC column by gradient elution, and the run time was 20 min. Matrix effect was evaluated and several dilutions of urine were tested in order to study the evolution of signal suppression. Spiked recovery studies demonstrated that the technique was both accurate (83.1–116.7%) and precise (RSD 1.4–15.6%). Finally, several clinical urine specimens without oxidative pretreatments were examined with the new technique and compared with previous reports.

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