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Experimental cell research 24건

  1. [해외논문]   IFC/ Editorial Board   SCI SCIE


    Experimental cell research v.361 no.1 ,pp. IFC - IFC , 2017 , 0014-4827 ,

    초록

    원문보기

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  2. [해외논문]   Translation of noncoding RNAs: Focus on lncRNAs, pri-miRNAs, and circRNAs   SCI SCIE

    Li, Lian-Ju (Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China ) , Leng, Rui-Xue (Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China ) , Fan, Yin-Guang (Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China ) , Pan, Hai-Feng (Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China ) , Ye, Dong-Qing (Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, China)
    Experimental cell research v.361 no.1 ,pp. 1 - 8 , 2017 , 0014-4827 ,

    초록

    Abstract Mammalian genome is pervasively transcribed, producing large number of noncoding RNAs (ncRNAs), including long noncoding RNAs (lncRNAs), primary miRNAs (pri-miRNA), and circular RNAs (circRNAs). The translation of these ncRNAs has long been overlooked. Increasing studies, however, based on ribosome profiling in various organisms provide important clues to unanticipated translation potential of lncRNAs. Moreover, a few functional peptides encoded by lncRNAs and pri-miRNAs underline the significance of their translation. Recently, several novel researches also evidence the translation of endogenous circRNAs. Given the functional significance exemplified by peptides translated by some ncRNAs and their pervasive translation, it is not too far-fetched to image that abnormal translation of ncRNAs may contribute to human diseases. Through challenging, deciphering ncRNA translation is required for comprehensive understanding of biology and medicine. In this review, we firstly present evidence concerning translation potential of lncRNAs and go on to introduce a few functional short peptides encoded by lncRNAs. Then, salient observations showing translation of pri-miRNAs and circRNAs are described in detail. We end by discussing the impact of ncRNA translation beyond producing peptides and referring briefly to the potential role of abnormal ncRNA translation in human diseases. Highlights Methods based on ribosome profiling highlight translation potential of lncRNAs. Peptides encoded lncRNAs and pri-miRNAs display functional importance. Translation of endogenous circRNAs has recently been discovered. The role of abnormal translation of ncRNAs in human disorders merits further investigation.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   IGF-I regulates HT1080 fibrosarcoma cell migration through a syndecan-2/Erk/ezrin signaling axis   SCI SCIE

    Mytilinaiou, Maria (Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece ) , Nikitovic, Dragana (Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece ) , Berdiaki, Aikaterini (Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece ) , Papoutsidakis, Antonis (Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece ) , Papachristou, Dionysios J. (Laboratory of Toxicology, School of Medicine, University of Crete, 71003 Heraklion, Greece ) , Tsatsakis, Aristidis (Laboratory of Anatomy-Histology-Embryology, Unit of Bone and Soft Tissue Studies, School of Medicine, University of Patras, Patras, Greece ) , Tzanakakis, George N. (Laboratory of Anatomy-Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece)
    Experimental cell research v.361 no.1 ,pp. 9 - 18 , 2017 , 0014-4827 ,

    초록

    Abstract Fibrosarcoma is a tumor of mesenchymal origin, originating from fibroblasts. IGF-I is an anabolic growth factor which exhibits significant involvement in cancer progression. In this study, we investigated the possible participation of syndecan-2 (SDC-2), a cell membrane heparan sulfate (HS) proteoglycan on IGF-I dependent fibrosarcoma cell motility. Our results demonstrate that SDC-2-deficient HT1080 cells exhibit attenuated IGF-I-dependent chemotactic migration (p IGF-I enhanced actin polymerization and ezrin/actin specific localization to cell membranes. Finally, treatment with IGF-I strongly increased SDC2 expression at both the mRNA and protein level (p contributes to actin polymerization and ezrin/actin specific localization to cell membranes, ultimately facilitating the progression of IGFI-dependent fibrosarcoma cell migration. Highlights IGF-I exhibits significant involvement in cancer progression. Syndecan-2 (SDC-2), has a discrete role in mesenchymal tumors progression. SDC2 facilitates IGF-I-dependent HT1080 fibrosarcoma cell chemotactic migration. SDC2 / IGF-IR / ezrin co-localization is demonstrated. Mediator co-localization enhances actin polymerization and membrane linking.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  4. [해외논문]   Gastric cancer tissue-derived mesenchymal stem cells impact peripheral blood mononuclear cells via disruption of Treg/Th17 balance to promote gastric cancer progression   SCI SCIE

    Wang, Mei (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Chen, Bin (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Sun, Xiao-Xian (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Zhao, Xiang-Dong (Zhenjiang Provincial Blood Center, Zhenjiang, Jiangsu, China ) , Zhao, Yuan-Yuan (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Sun, Li (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Xu, Chang-Gen (Zhenjiang Provincial Blood Center, Zhenjiang, Jiangsu, China ) , Shen, Bo (Department of Oncology, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, China ) , Su, Zhao-Liang (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Xu, Wen-Rong (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China ) , Zhu, Wei (School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China)
    Experimental cell research v.361 no.1 ,pp. 19 - 29 , 2017 , 0014-4827 ,

    초록

    Abstract Gastric cancer tissue-derived mesenchymal stem cells (GC-MSCs) are important resident stromal cells in the tumor microenvironment (TME) and have been shown to play a key role in gastric cancer progression. Whether GC-MSCs exert a tumor-promoting function by affecting anti-tumor immunity is still unclear. In this study, we used GC-MSC conditioned medium (GC-MSC-CM) to pretreat peripheral blood mononuclear cells (PBMCs) from healthy donors. We found that GC-MSC-CM pretreatment markedly reversed the inhibitory effect of PBMCs on gastric cancer growth in vivo, but did not affect functions of PBMCs on gastric cancer cell proliferation, cell cycle and apoptosis in vitro . PBMCs pretreated with GC-MSC-CM significantly promoted gastric cancer migration and epithelial-mesenchymal transition in vitro and liver metastases in vivo . Flow cytometry analysis showed that GC-MSC-CM pretreatment increased the proportion of Treg cells and reduced that of Th17 cells in PBMCs. CFSE labeling and naIve CD4 + T cells differentiation analysis revealed that GC-MSC-CM disrupted the Treg/Th17 balance in PBMCs by suppressing Th17 cell proliferation and inducing differentiation of Treg cells. Overall, our collective results indicate that GC-MSCs impair the anti-tumor immune response of PBMCs through disruption of Treg/Th17 balance, thus providing new evidence that gastric cancer tissue-derived MSCs contribute to the immunosuppressive TME. Highlights GC-MSCs educate PBMCs to promote gastric cancer progression. GC-MSCs disrupt the Treg/Th17 balance in PBMCs. GC-MSCs induce Treg cell differentiation and suppress Th17 cell proliferation.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  5. [해외논문]   RETRACTED: The long noncoding RNA CASC2 inhibits tumorigenesis through modulating the expression of PTEN by targeting miR-18a-5p in esophageal carcinoma   SCI SCIE

    Zhang, Wenjing (<!--organization-->School of Basic Medicine Sciences, Henan University of Chinese Medicine<!--/organization-->, <!--city-->Zhengzhou<!--/city--><!--postal-code-->450046<!--/postal-code-->, <!--country-->China<!--/country-->) , He, Wei (<!--organization-->Department of Oncology, The First Affiliated Hospital of Zhengzhou University<!--/organization-->, <!--city-->Zhengzhou<!--/city-->, <!--country-->China<!--/country-->) , Gao, Jianfeng (<!--organization-->School of Basic Medicine Sciences, Henan University of Chinese Medicine<!--/organization-->, <!--city-->Zhengzhou<!--/city--><!--postal-code-->450046<!--/postal-code-->, <!--country-->China<!--/country-->) , Wang, Yuanyuan (<!--organization-->School of Basic Medical Sciences, Zhengzhou University<!--/organization-->, <!--city-->Zhengzhou<!--/city-->, <!--country-->China<!--/country-->) , Zang, Wenqiao (<!--organization-->School of Basic Medical Sciences, Zhengzhou University<!--/organization-->, <!--city-->Zhengzhou<!--/city-->, <!--country-->China<!--/country-->) , Dong, Ziming (<!--organization-->School) , Zhao, Guoqiang
    Experimental cell research v.361 no.1 ,pp. 30 - 38 , 2017 , 0014-4827 ,

    초록

    Abstract Gastric cancer tissue-derived mesenchymal stem cells (GC-MSCs) are important resident stromal cells in the tumor microenvironment (TME) and have been shown to play a key role in gastric cancer progression. Whether GC-MSCs exert a tumor-promoting function by affecting anti-tumor immunity is still unclear. In this study, we used GC-MSC conditioned medium (GC-MSC-CM) to pretreat peripheral blood mononuclear cells (PBMCs) from healthy donors. We found that GC-MSC-CM pretreatment markedly reversed the inhibitory effect of PBMCs on gastric cancer growth in vivo, but did not affect functions of PBMCs on gastric cancer cell proliferation, cell cycle and apoptosis in vitro . PBMCs pretreated with GC-MSC-CM significantly promoted gastric cancer migration and epithelial-mesenchymal transition in vitro and liver metastases in vivo . Flow cytometry analysis showed that GC-MSC-CM pretreatment increased the proportion of Treg cells and reduced that of Th17 cells in PBMCs. CFSE labeling and naIve CD4 + T cells differentiation analysis revealed that GC-MSC-CM disrupted the Treg/Th17 balance in PBMCs by suppressing Th17 cell proliferation and inducing differentiation of Treg cells. Overall, our collective results indicate that GC-MSCs impair the anti-tumor immune response of PBMCs through disruption of Treg/Th17 balance, thus providing new evidence that gastric cancer tissue-derived MSCs contribute to the immunosuppressive TME. Highlights GC-MSCs educate PBMCs to promote gastric cancer progression. GC-MSCs disrupt the Treg/Th17 balance in PBMCs. GC-MSCs induce Treg cell differentiation and suppress Th17 cell proliferation.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [해외논문]   CXCR5+ CD8+ T cells potently infiltrate pancreatic tumors and present high functionality   SCI SCIE

    Bai, Minghui (The Second Ward, Department of Hepatobiliary and Hernia Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China ) , Zheng, Youwei (Department of Hepatobiliary and Hernia Surgery, The First Affiliated Hospital of Henan Science and Technology Univeristy, Luoyang, Henan, China ) , Liu, Haichao (The Second Ward, Department of Hepatobiliary and Hernia Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China ) , Su, Baowei (The Second Ward, Department of Hepatobiliary and Hernia Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China ) , Zhan, Yong (The Second Ward, Department of Hepatobiliary and Hernia Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China ) , He, Hua (The Second Ward, Department of Hepatobiliary and Hernia Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China)
    Experimental cell research v.361 no.1 ,pp. 39 - 45 , 2017 , 0014-4827 ,

    초록

    Abstract Despite continued improvement in conventional therapy, pancreatic cancer continues to be one of the deadliest tumors worldwide with abysmal 5-year survival rate. New immunotherapeutic strategies that aim at improving antitumor cytotoxic CD8 + T cell responses are being developed in solid tumors. To assist the development of immunotherapies, we investigated the CD8 + T cells in pancreatic cancer patients. Compared to healthy individuals, pancreatic cancer patients presented a significant enrichment in the frequency of CD8 + CXCR5 + T cells. In the tumor microenvironment, the frequencies of CD8 + CXCR5 + T cells were further increased. In most cases, over half of tumor-infiltrating CD8 + T cells were CD8 + CXCR5 + T cells. Compared to the circulating population, the tumor-infiltrating CD8 + CXCR5 + T cells expressed higher levels of PD-1 and TIM-3. Functional analyses demonstrated that upon CD3/CD28 activation, the percentages of TNF-expressing and IFN-γ-expressing cells in CD8 + CXCR5 + T cells were significantly higher than that in CD8 + CXCR5 - T cells. CD8 + CXCR5 + T cells also presented enhanced cytotoxicity than CD8 + CXCR5 - T cells. Upon PD-1 and TIM-3 blockade, the functions of CD8 + CXCR5 + T cells were further improved. The disease-free survival of pancreatic cancer patients following tumor resection was positively correlated with the frequencies of circulating and tumor-infiltrating CD8 + CXCR5 + T cells. Together, our study identified that CD8 + CXCR5 + T cells were a potent subset of CD8 + T cells that were highly enriched in pancreatic cancer patients and could respond to anti-PD-1/anti-TIM-3 blockade by further upregulation in function.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   Treponema pallidum flagellins stimulate MMP-9 and MMP-13 expression via TLR5 and MAPK/NF-κB signaling pathways in human epidermal keratinocytes   SCI SCIE

    Jiang, Chuanhao (Institute of Pathogenic Biology, Medical College, University of South China) , Xu, Man (Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control) , Kuang, Xingxing (Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China ) , Xiao, Jinhong (Institute of Pathogenic Biology, Medical College, University of South China) , Tan, Manyi (Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control) , Xie, Yafeng (Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China ) , Xiao, Yongjian (Institute of Pathogenic Biology, Medical College, University of South China) , Zhao, Feijun (Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control) , Wu, Yimou (Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China )
    Experimental cell research v.361 no.1 ,pp. 46 - 55 , 2017 , 0014-4827 ,

    초록

    Abstract Syphilis is a chronic disease caused by Treponema pallidum and the pathogenesis is still unclear. T. pallidum infection induced inflammatory responses are involved in the immunopathological damage in skin and other tissues. Flagellin, the monomeric subunit of bacterial flagella, is a classic pathogen associated molecular patterns (PAMPs) that interacts to TLR5 and induces inflammatory responses. Keratinocytes, as immune sentinels recognize the PAMPs via TLRs, play an important role in skin innate immune response. Matrix metalloproteinases (MMPs) expressed by keratinocytes are involved in skin inflammatory responses and promoting pathogens invasion. In this study, we demonstrate that FlaB1, FlaB2 and FlaB3, the flagellins of T. pallidum , induced MMP-9 and MMP-13 production in human immortalized keratinocytes cell line HaCaT. Silencing of TLR5, but not TLR2 and TLR4 attenuated MMP-9 and MMP-13 expressions induced by T. pallidum flagellins. MMP-9 and MMP-13 expressions were also be abrogated by transfection with a dominant negative (DN) plasmid of MyD88. We also found that treatment of HaCaT cells with FlaB1, FlaB2 and FlaB3 activate the MAPK and NF-κB signaling pathways. Inhibited of ERK, JNK, p38 and NF-κB suppressed MMP-9 expression induced by the FlaB1. MMP-13 expression was found to be suppressed by pretreatment with inhibitors of ERK, JNK and NF-κB, but not p38. These findings demonstrate that T. pallidum flagellins (FlaB1, FlaB2 or FlaB3) can stimulate MMP-9 and MMP-13 expression through TLR5 and MAPK/NF-κB signaling pathways in human epidermal keratinocytes, which could contribute to the pathogenesis of T. pallidum infection. Highlights T. pallidum flagellins induced MMPs expression in HaCaT cells via TLR5/MyD88 pathway. MAPK and NF-κB are involved in T. pallidum flagellins induced MMPs expression. MMPs may contribute to skin inflammatory response during T. pallidum infection.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [해외논문]   T cell responses in senior patients with community-acquired pneumonia related to disease severity   SCI SCIE

    Bian, Lu-Qin (Department of Special Procurement Ward, The First Affiliated Hospital of Soochow University, Suzhou 215006 China ) , Bi, Ying (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Zhou, Shao-Wei (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Chen, Zi-Dan (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Wen, Jun (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Shi, Jin (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Mao, Ling (Department of Pneumoconiosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433 China ) , Wang, Ling (Department of Special Procurement Ward, The First Affiliated Hospital of Soochow University, Suzhou 215006 China)
    Experimental cell research v.361 no.1 ,pp. 56 - 62 , 2017 , 0014-4827 ,

    초록

    Abstract Senior individuals older than 65 years of age are at a disproportionally higher risk of developing pneumonia. Impaired capacity to defend against airway infections may be one of the reasons. It is generally believed that weaker regulatory T cell responses may be beneficial to host defense against pathogens. In senior patients with community-acquired bacterial pneumonia, we investigated the frequencies and functions of regulatory T cells. Interestingly, we found that compared to age- and sex-matched healthy controls, senior pneumonia patients presented lower frequencies of Foxp3-expressing and Helios-expressing CD4 + T cells. The quantity of Foxp3 and Helios being expressed, measured by their mRNA transcription levels, was also lower in CD4 + T cells from pneumonia patients. Furthermore, following TCR and TGF-β stimulation, pneumonia patients presented impaired capacity to upregulate Foxp3 and Helios. Functional analyses revealed that CD4 + T cells from pneumonia patients secreted lower amounts of IL-10 and TGF-β, two cytokines critical to regulatory T cell-mediated suppression. Also, the expression of granzyme B and perforin, which were cytolytic molecules potentially utilized by regulatory T cells to mediate the elimination of antigen-presenting cells and effector T cells, were reduced in CD4 + CD25 + T cells from senior pneumonia patients. In addition, the CD4 + CD25 + T cells from senior pneumonia patients presented reduced capacity to suppress effector CD4 + and CD8 + T cell proliferation. Moreover, the value of pneumonia severity index was inversely correlated with several parameters of regulatory T cell function. Together, our results demonstrated that senior pneumonia patients presented a counterintuitive impairment in regulatory T cell responses that was associated with worse prognosis.

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    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   The crosstalk between Sirt1 and Keap1/Nrf2/ARE anti-oxidative pathway forms a positive feedback loop to inhibit FN and TGF-β1 expressions in rat glomerular mesangial cells   SCI SCIE

    Huang, Kaipeng (Drug Clinical Trial Institution, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, China ) , Gao, Xiang (Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China ) , Wei, Wentao (Institute of Drug Synthesis and Pharmaceutical Process, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China)
    Experimental cell research v.361 no.1 ,pp. 63 - 72 , 2017 , 0014-4827 ,

    초록

    Abstract Oxidative stress aroused by advanced glycation-end products (AGEs) is a culprit in the pathological progression of diabetic nephropathy. Both Sirt1 and the Keap1/Nrf2/ARE anti-oxidative pathway exert crucial inhibitory effects on the development of diabetic nephropathy. Our previous study has confirmed that Sirt1 activation can inhibit the upregulation of fibronectin (FN) and transforming growth factor-β1 (TGF-β1) by promoting Keap1/Nrf2/ARE pathway in glomerular mesangial cells (GMCs) challenged with AGEs. However, the underlying mechanism needs further investigation. Here, we found that concomitant with deacetylating and reducing the ubiquitination levels of Nrf2, Sirt1 significantly enhanced the activity of Keap1/Nrf2/ARE pathway including decreasing Keap1 expression, promoting the nuclear content, ARE-binding ability, and transcriptional activity of Nrf2, augmenting the protein levels of heme oxygenase 1, a target gene of Nrf2, which eventually quenched ROS overproduction and alleviating FN and TGF-β1 accumulation in AGEs-treated GMCs. And depletion of Nrf2 blocked those renoprotective effects of Sirt1. Interestingly, Nrf2 also positively regulated Sirt1 at the protein expression and deacetylase activity levels as evidenced by tert -Butylhydroquinone and specific siRNA targeting Nrf2 to downregulate FN and TGF-β1. In conclusion, the current study basically demonstrated that the crosstalk between Sirt1 and Keap1/Nrf2/ARE anti-oxidative pathway forms a positive feedback loop to inhibit the protein expressions of FN and TGF-β1 in AGEs-treated GMCs. Highlights Sirt1 deacetylated and reduced the ubiquitination level of Nrf2 in AGEs-treated GMCs. Sirt1 promoted Keap1/Nrf2/ARE pathway activation to decrease FN and TGF-β1 levels. Nrf2 also regulated Sirt1 at protein expression and deacetylase activity levels. Sirt1 and Keap1/Nrf2/ARE pathway forms a positive feedback to prevent FN and TGF-β1 expressions.

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  10. [해외논문]   Robo signaling regulates the production of cranial neural crest cells   SCI SCIE

    Li, Yan (Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China ) , Zhang, Xiao-tan (Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China ) , Wang, Xiao-yu (Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China ) , Wang, Guang (Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China ) , Chuai, Manli (Division of Cell and Developmental Biology, University of Dundee, Dundee DD1 5EH, UK ) , Mü (School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK ) , nsterberg, Andrea (Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, C) , Yang, Xuesong
    Experimental cell research v.361 no.1 ,pp. 73 - 84 , 2017 , 0014-4827 ,

    초록

    Abstract Slit/Robo signaling plays an important role in the guidance of developing neurons in developing embryos. However, it remains obscure whether and how Slit/Robo signaling is involved in the production of cranial neural crest cells. In this study, we examined Robo1 deficient mice to reveal developmental defects of mouse cranial frontal and parietal bones, which are derivatives of cranial neural crest cells. Therefore, we determined the production of HNK1 + cranial neural crest cells in early chick embryo development after knock-down (KD) of Robo1 expression. Detection of markers for pre-migratory and migratory neural crest cells, PAX7 and AP-2α, showed that production of both was affected by Robo1 KD. In addition, we found that the transcription factor slug is responsible for the aberrant delamination/EMT of cranial neural crest cells induced by Robo1 KD, which also led to elevated expression of E- and N-Cadherin. N-Cadherin expression was enhanced when blocking FGF signaling with dominant-negative FGFR1 in half of the neural tube. Taken together, we show that Slit/Robo signaling influences the delamination/EMT of cranial neural crest cells, which is required for cranial bone development. Highlights Development of the mouse craniofacial skeleton is affected in absence of Robo1. Slit/Robo signaling influences the delamination/EMT of cranial neural crest cells. Robo1 KD promoted expression of adhesion molecules in chick neural tube.

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