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Archives of insect biochemistry and physiology 10건

  1. [해외논문]   Issue information  


    Archives of insect biochemistry and physiology v.98 no.4 ,pp. NA , 2018 , 0739-4462 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Effect of the insect phenoloxidase on the metabolism of l‐DOPA  

    Wu, Kai (College of Life Sciences, Shangrao Normal University, Shangrao, China) , Han, Fang (Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China) , Yuan, Yi (Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China) , Liu, Yining (Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China) , Ling, Erjun (Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences,) , Wang, Qian , Huang, Wuren
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21457 , 2018 , 0739-4462 ,

    초록

    Abstract Insect prophenoloxidase (PPO) induces melanization around pathogens. Before melanization, PPO is cleaved into phenoloxidase (PO) by serine proteases. Insect PPO can also be activated by exogenous proteases secreted by pathogens as well as by other compounds, such as ethanol and cetylpyridinium chloride (CPC). However, the effect of these activators on the activity of PO is unclear. In this study, the insect endogenous serine protease AMM1, α‐chymotrypsin, and ethanol were used to activate recombinant Drosophila PPO1 (rPPO1), and the PO activity differed depending on the activator applied. The PO‐induced intermediates during melanization also varied markedly in their numbers and abundances. Therefore, this study indicates that the mechanism of PPO activation influences PO activity. It also suggests that PO‐induced different intermediates may affect the antibacterial activity during melanization due to their toxicity.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Cellular energy metabolism maintains young status in old queen honey bees (Apis mellifera)  

    Lu, Cheng‐ (Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao‐Yuan, Taiwan) , Yen (Department of Biomedical Sciences, Chang Gung University, Tao‐Yuan, Taiwan) , Qiu, Jiantai Timothy (Department of Biomedical Sciences, Chang Gung University, Tao‐Yuan, Taiwan) , Hsu, Chin‐ , Yuan
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21468 , 2018 , 0739-4462 ,

    초록

    Abstract Trophocytes and oenocytes of queen honey bees are used in studies of cellular longevity, but their cellular energy metabolism with age is poorly understood. In this study, the molecules involved in cellular energy metabolism were evaluated in the trophocytes and oenocytes of young and old queen bees. The findings indicated that there were no significant differences between young and old queen bees in β‐oxidation, glycolysis, and protein synthesis. These results indicate that the cellular energy metabolism of trophocytes and oenocytes in old queen bees is similar to young queen bees and suggests that maintaining cellular energy metabolism in a young status may be associated with the longevity of queen bees. Fat and glycogen accumulation increased with age indicating that old queen bees are older than young queen bees.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  4. [해외논문]   Maintenance of redox balance by antioxidants in hemolymph of the greater wax moth Galleria mellonella larvae during encapsulation response  

    Grizanova, Ekaterina V. (Novosibirsk State Agrarian University, Novosibirsk, Russia ) , Semenova, Alexandra D. (Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia ) , Komarov, Denis A. (Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia ) , Chertkova, Ekaterina A. (Institute of Systematic and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia ) , Slepneva, Irina A. (Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia ) , Dubovskiy, Ivan M. (Novosibirsk State Agrarian University, Novosibirsk, Russia)
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21460 , 2018 , 0739-4462 ,

    초록

    Abstract The lipid peroxidation process in hemocytes, activities of phenoloxidase and key enzymatic antioxidants (superoxide dismutase, glutathione‐S‐transferase, catalase) and nonenzymatic antioxidants (thiols, ascorbate) in hemolymph of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) were studied during the encapsulation process of nylon implants. It has been established that as soon as 15 min after piercing a cuticle with the implant, a capsule is formed on its surface. Active melanization of the capsule has been shown to last for 4 h. During the first hours after incorporating the implant, an increase in phenoloxidase activity and lipid peroxidation in the insect hemocytes has been revealed. Adhesion and degranulation on the surface of foreign object lead to the depletion of total hemocytes count (THC). Our results indicated that thiols and ascorbate molecules take part in the immediate antioxidant response, during later stages of encapsulation process hemolymph glutathione‐S‐transferase detoxifies and protects insect organism thereby restoring the internal redox balance. We suggest that nonenzymatic and enzymatic antioxidants of hemolymph plasma play a key role in the maintenance of redox balance during encapsulation of foreign targets.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Issue Information  


    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21416 , 2018 , 0739-4462 ,

    초록

    Abstract The lipid peroxidation process in hemocytes, activities of phenoloxidase and key enzymatic antioxidants (superoxide dismutase, glutathione‐S‐transferase, catalase) and nonenzymatic antioxidants (thiols, ascorbate) in hemolymph of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) were studied during the encapsulation process of nylon implants. It has been established that as soon as 15 min after piercing a cuticle with the implant, a capsule is formed on its surface. Active melanization of the capsule has been shown to last for 4 h. During the first hours after incorporating the implant, an increase in phenoloxidase activity and lipid peroxidation in the insect hemocytes has been revealed. Adhesion and degranulation on the surface of foreign object lead to the depletion of total hemocytes count (THC). Our results indicated that thiols and ascorbate molecules take part in the immediate antioxidant response, during later stages of encapsulation process hemolymph glutathione‐S‐transferase detoxifies and protects insect organism thereby restoring the internal redox balance. We suggest that nonenzymatic and enzymatic antioxidants of hemolymph plasma play a key role in the maintenance of redox balance during encapsulation of foreign targets.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  6. [해외논문]   Cover Image  

    Huang, Qing‐ , Ting , Ma, Hai‐ , Hao , Deng, Xi‐ , Le , Zhu, Hang , Liu, Jia , Zhou, Yong , Zhou, Xiao‐ , Mao
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21498 , 2018 , 0739-4462 ,

    초록

    On the cover : The cover image, by Qing‐Ting Huang et al., is based on the Article Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella , DOI: 10.1002/arch.21466 .

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   Influence of aminergic and peptidergic substances on heart beat frequency in the stick insect Carausius morosus (Insecta, Phasmatodea)  

    Marco, Heather G. (Department of Biological Sciences, University of Cape Town, Rondebosch, South Africa) , Katali, Ottilie K. H. (Department of Biological Sciences, University of Cape Town, Rondebosch, South Africa) , Gä (Department of Biological Sciences, University of Cape Town, Rondebosch, South Africa) , de, Gerd
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21469 , 2018 , 0739-4462 ,

    초록

    Abstract The dorsal heart of the Indian stick insect, Carausius morosus , is responsible for the anterograde flow of hemolymph to the aorta and into the body cavity. The contraction frequency of the insect heart is known to be influenced by several substances of neural source. Here, a semi‐exposed heart assay was employed to study the effect of an aminergic substance (octopamine) and three neuropeptides ( C. morosus hypertrehalosemic hormone [Carmo‐HrTH], crustacean cardioactive peptide [CCAP], and proctolin) on heart contraction. The contraction frequency was measured as beats per minute in adults ligated between the head and the prothorax. All three investigated neuropeptides had a stimulatory effect on heart contraction that lasted approximately 6 min, after which the normal heart beat rate was restored. Proctolin and CCAP stimulated the rate of heart beat also in unligated stick insects, whereas Carmo‐HrTH was active only in ligated insects. The latter could suggest that when the stick insect is not ligated, a competing substance may be released from the head of C. morosus ; the competing substance is, apparently, not physiologically active but it binds or blocks access to the receptor of Carmo‐HrTH‐II, thereby rendering the HrTH peptide “not active.” In ligated stick insects, 6.7 × 10 −8 M Carmo‐HrTH‐II significantly increased the heart beat rate; higher doses resulted in no further increase, suggesting the saturation of the HrTH receptor. Octopamine inhibited the rate at which the heart contracted in a dose‐dependent manner; inhibition was achieved with 10 −4 M of octopamine.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Overexpression of antimicrobial peptides contributes to aging through cytotoxic effects in Drosophila tissues  

    Badinloo, Marziyeh (Southern Methodist University, Dallas, Texas ) , Nguyen, Elizabeth (Southern Methodist University, Dallas, Texas ) , Suh, Winston (Southern Methodist University, Dallas, Texas ) , Alzahrani, Faisal (Southern Methodist University, Dallas, Texas ) , Castellanos, Jovelyn (Southern Methodist University, Dallas, Texas ) , Klichko, Vladimir I. (Southern Methodist University, Dallas, Texas ) , Orr, William C. (Southern Methodist University, Dallas, Texas ) , Radyuk, Svetlana N. (Southern Methodist University, Dallas, Texas)
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21464 , 2018 , 0739-4462 ,

    초록

    Abstract The innate immune response tends to become hyperactive and proinflammatory in older organisms. We investigated connections between activity of the immune‐related genes and aging using the Drosophila model. A hallmark of Drosophila immunity is the production of antimicrobial peptides (AMP), whose expression is triggered via activation of the Toll and Imd immune pathways and regulated by NF‐ĸB‐like transcription factors, Dif/Dorsal and Relish. It was previously shown that overexpression of the upstream component of the immune pathways shortens lifespan via activation of the Relish‐dependent immune response. Here we show that direct overexpression of the Relish target AMP genes broadly at high levels or in the fat body induced apoptosis, elicited depolarization of the mitochondria and significantly shortened lifespan. Underexpression of Relish in the fat body beginning in the second half of lifespan prevented overactivation of AMPs and extended longevity. Unlike infection‐induced responses, the age‐related increase in AMPs does not require the upstream recognition/transduction module of the Imd pathway. It does however require downstream elements, including Relish and Ird5, a component of the downstream IKK complex. Together, these results established causal links between high‐level production of antimicrobial peptides and longevity.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

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  9. [해외논문]   Editorial Note  

    Kim, Yonggyun
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21494 , 2018 , 0739-4462 ,

    초록

    Abstract The innate immune response tends to become hyperactive and proinflammatory in older organisms. We investigated connections between activity of the immune‐related genes and aging using the Drosophila model. A hallmark of Drosophila immunity is the production of antimicrobial peptides (AMP), whose expression is triggered via activation of the Toll and Imd immune pathways and regulated by NF‐ĸB‐like transcription factors, Dif/Dorsal and Relish. It was previously shown that overexpression of the upstream component of the immune pathways shortens lifespan via activation of the Relish‐dependent immune response. Here we show that direct overexpression of the Relish target AMP genes broadly at high levels or in the fat body induced apoptosis, elicited depolarization of the mitochondria and significantly shortened lifespan. Underexpression of Relish in the fat body beginning in the second half of lifespan prevented overactivation of AMPs and extended longevity. Unlike infection‐induced responses, the age‐related increase in AMPs does not require the upstream recognition/transduction module of the Imd pathway. It does however require downstream elements, including Relish and Ird5, a component of the downstream IKK complex. Together, these results established causal links between high‐level production of antimicrobial peptides and longevity.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [해외논문]   Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella  

    Huang, Qing‐ (Long Ping Branch, Graduate School of Hunan University, Changsha, China) , Ting (Hunan Academy of Agricultural Sciences, Changsha, China) , Ma, Hai‐ (Hunan Academy of Agricultural Sciences, Changsha, China) , Hao (Hunan Academy of Agricultural Sciences, Changsha, China) , Deng, Xi‐ (Hunan Academy of Agricultural Sciences, Changsha, China) , Le (Hunan Academy of Agricultural Sciences, Changsha, China) , Zhu, Hang (Long Ping Branch, Graduate School of Hunan University, Changsha, China) , Liu, Jia , Zhou, Yong , Zhou, Xiao‐ , Mao
    Archives of insect biochemistry and physiology v.98 no.4 ,pp. e21466 , 2018 , 0739-4462 ,

    초록

    Abstract The β‐adrenergic‐like octopamine receptor (OA2B2) belongs to the class of G‐protein coupled receptors. It regulates important physiological functions in insects, thus is potentially a good target for insecticides. In this study, the putative open reading frame sequence of the Pxoa2b2 gene in Plutella xylostella was cloned. Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. PxOA2B2 was transiently expressed in HEK‐293 cells. It was found that PxOA2B2 could be activated by both octopamine and tyramine, resulting in increased intracellular cyclic AMP (cAMP) levels, whereas dopamine and serotonin were not effective in eliciting cAMP production. Further studies with series of PxOA2B2 agonists and antagonists showed that all four tested agonists (e.g., naphazoline, clonidine, 2‐phenylethylamine, and amitraz) could activate the PxOA2B2 receptor, and two of tested antagonists (e.g., phentolamine and mianserin) had significant antagonistic effects. However, antagonist of yohimbine had no effects. Quantitative real‐time polymerase chain reaction analysis showed that Pxoa2b2 gene was expressed in all developmental stages of P. xylostella and that the highest expression occurred in male adults. Further analysis with fourth‐instar P. xylostella larvae showed that the Pxoa2b2 gene was mainly expressed in Malpighian tubule, epidermal, and head tissues. This study provides both a pharmacological characterization and the gene expression patterns of the OA2B2 in P. xylostella , facilitating further research for insecticides using PxOA2B2 as a target.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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