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Archives of pharmacal research : a publication of ... 23건

  1. [국내논문]   Effects of Radioprotectors on DNA Repair Capacity of Tumor Cells  

    Kim, Choon-Mi (College of Pharmacy, Ewha Womans University ) , Kim, Mi-Kyung (College of Pharmacy, Ewha Womans University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 259 - 264 , 1993 , 0253-6269 ,

    초록

    Three cell lines, CHO, L929 and B16 which are non-tumorigenic and cancer cells, respectively, were first tested for their survival in the presence of radioprotective ginseng protein fraction(GPF0. The influence of three radioprotectors-CPF, cysteamine, and 1-Methyl-2-bis[(2-methylthio)vinyl] quinolinium iodide (MVQI) on DNA repair capacity of UV damaged cells survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was also investigated by measuring $^3H$ -thymidine incorporation of PUVA treated cells. In cell survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was not high enough to consider it as an antitumorigenic agent. Variable results were obtained in the effects on DNA repair capacity depending on the protectors and cell lines used. In pretreatment, the presence of GPF and MVOI brought about a sinificant increase in the capacity in both CHO and B16 cells. However, in L929, the enhancing effect was not shown. In all three cell lines, cysteamine showed lower repair capacity than control, suggesting the primary damage reduction in stronger enhancing effects in L929 and B16 cells, while it was weaker in CHO cells. Here also cystemine hsowed a very little or no increase in the capacity in all three cell lines. These results demonstrate that GPF has mild cytotoxicity in tumorignic cells and that GPF and MVQI enhance DNA repair capacity of UV damaged cells, whether they are tumorigenic or not. On the other hand, cysteamine shows only damage reduction effect. Celles of different genetic origin seem to give different responses to the modifier and different modifiers may possibly work by different mechanisms.

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  2. [국내논문]   A New Synthesis of 6,10,14,18-Tetramethyl-5,9,13,17 (E,E,E)-nonadecatetraen-2-one  

    Park, Oee-Sook (Department of Chemistry, Chungbuk National University ) , Ahn, Su-Kyung (Department of Chemistry, Chungbuk National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 265 - 270 , 1993 , 0253-6269 ,

    초록

    6, 10, 14, 18-Tetramethyl-5, 9, 131, 17(E, E, E)-nonadecatetraen-2-one was synthesized from geraniol or 6-methyl-5(E)-hepten-2-one in 6 steps, repectively. The key step in both syntheses, was the condensation of phenyl sulfone compound and allylic chloride.

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  3. [국내논문]   Design and Cloning of the Gene for a Novel Insulin Analogue, $(B^{30}$-Homoserine) Human Insulin   피인용횟수: 1

    Nam, Doo-H. (College of Pharmacy, Yeungnam University ) , Ko, Jeong-Heon (Molecular Biology Laboratory, Genetic Engineering Research Institute ) , Lee, Seung-Yup (College of Pharmacy, Yeungnam University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 271 - 275 , 1993 , 0253-6269 ,

    초록

    In order to prepare a novel human insulin analogue suhbstituted with homoserine at B $^{30}$ / position, (B $^{30}$ /-homoserine) human insulin, a synthetic gene was designed by linking directly a gene for B chain with that for A chain. This gene was constructed by enzymatic joining of 10 different synthetic oligonucleotides, and then inserted at the polylinker region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique was employed using amino terminal regions of lacZ gene up to Clal or hpal, and either of them has been located under tac promoter. The chemical induction of these fused genes by isopropyl-.betha.-D-thiogalactopyranoside (IPTG) gave a satisfactory level of expression in Escherichia coli harboring the ocnstructed plasmids. It was observed that the fused gene product as a single chain insulin precusor was produced more than 30% of total cell protein of E. coli as a form of inclusion body.

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  4. [국내논문]   Effect of 3-Methylcholanthrene on Rat Uterus: Uterine Growth and Mechanism of Action of 3-Methylcholanthrene  

    Sheen, Yhun-Y. (College of Pharmacy, Ewha Womans University ) , Kim, Sun-S. (College of Pharmacy, Ewha Womans University ) , Yun, Hea-C. (College of Pharmacy, Ewha Womans University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 276 - 282 , 1993 , 0253-6269 ,

    초록

    This study has been undertaken to examine the effect of 3-methylcholanthrene (3MC) on rat uterine growth and to understand the mechanism of action of 3MC in rat uterus. After diethylstilbesterol(DES) or tamoxifen(TAM) or 3MC or DES plus TAM or DES plus 3MC was administered into immature female rats, uterine weight over corn oil-treated uteri. 3MC treatment had no effect on uterine weight but, DES stimulated uterine weight was inhibited by 3MC concomitant tratment. While TAM alone treatment showed slight increase in uterine wieght, inhibited uterine growth simulated by DES when it was adiministrated with DES condirect binding assay with $[^3H]$ estradiol and the relative binding affinities of 3MC and TAM were estimated by competetion assy. Estradiol tumed out to have high affinity for rat uterine estrogen receptor (kd = 0.4 nM). The relative binding affinities of TAM and 3MC were 1% and 4.7% that of DES for rat uterine estrogen receptor, respectively. 3MC was shown to have similar affinity for eat uterine estrogen receptor to that of TAM. Effects of DES 3MC and TAM administration in vivo on rat uterine estrogen recptor level were examined. It was confirmed that the estrogen, DES and antiestrogen, TAM decreased estrogen receptor levels from rat ulterus and also 3MC decreased rat uterine estrogen receptor level when rats were treated with DES, TAM and 3MC in vivo. Data indicates that 3MC acts as an antiestrogen mediated through estrogen receptor system.

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  5. [국내논문]   Evaluation of some flavonoids as potential bradykinin antagonists  

    Yun-Choi, Hye Sook , Chung, Sung Hyun , Kim, Young Joo
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 283 - 288 , 1993 , 0253-6269 ,

    초록

    This study has been undertaken to examine the effect of 3-methylcholanthrene (3MC) on rat uterine growth and to understand the mechanism of action of 3MC in rat uterus. After diethylstilbesterol(DES) or tamoxifen(TAM) or 3MC or DES plus TAM or DES plus 3MC was administered into immature female rats, uterine weight over corn oil-treated uteri. 3MC treatment had no effect on uterine weight but, DES stimulated uterine weight was inhibited by 3MC concomitant tratment. While TAM alone treatment showed slight increase in uterine wieght, inhibited uterine growth simulated by DES when it was adiministrated with DES condirect binding assay with $[^3H]$ estradiol and the relative binding affinities of 3MC and TAM were estimated by competetion assy. Estradiol tumed out to have high affinity for rat uterine estrogen receptor (kd = 0.4 nM). The relative binding affinities of TAM and 3MC were 1% and 4.7% that of DES for rat uterine estrogen receptor, respectively. 3MC was shown to have similar affinity for eat uterine estrogen receptor to that of TAM. Effects of DES 3MC and TAM administration in vivo on rat uterine estrogen recptor level were examined. It was confirmed that the estrogen, DES and antiestrogen, TAM decreased estrogen receptor levels from rat ulterus and also 3MC decreased rat uterine estrogen receptor level when rats were treated with DES, TAM and 3MC in vivo. Data indicates that 3MC acts as an antiestrogen mediated through estrogen receptor system.

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  6. [국내논문]   Evaluation of Some Flavonoids as Potential Bradykinin Antagonists  

    Choi, Hye-Sook (Natural Products Research Institute, Seoul National University ) , Chung, Sung-Hyun (College of Pharmacy, Kyung Hee University ) , Kim, Young-Joo (Natural Products Research Institute, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 283 - 288 , 1993 , 0253-6269 ,

    초록

    Fourteen flavonoids were evaluated for their effects as potential bradykinin (BK) antagonists. The compounds were evaluatd in several in vitro and in vivo (oral administration) systems ; inhibition of BK induced contractions in isolated rat ileum and uterus, antagonistic effects of BK induced plasma extravasation, reduction of acetic acid induced withing nociception and protection from endotoxic shock. Skullcapflavone II (3), baicalein (5), 5-methoxyflavone (11), 6-methoxyflavone (12) and 2'-methoxyflavone (14) showed effects in all the tests although the order of potency were somewhat varied.

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  7. [국내논문]   Binding of Sanjoinine-A (Frangufoline) to Calmodulin  

    Han, Yong-Nam (Natural Products Research Institute, Seoul National University ) , Kim, Geum-Yi (Natural Products Research Institute, Seoul National University ) , Hwang, Keum-Hee (Natural Products Research Institute, Seoul National University ) , Han, Byung-Hoon (Natural Products Research Institute, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 289 - 294 , 1993 , 0253-6269 ,

    초록

    A binding protein of radio-labeled sanjoinine-A (fangufoline) in rat brain cytoplasm was investigated, using an equilibrium dialysis technique. The labeled agent was bound to the cytosol fraction with two distinctly different types of sets in calclum ion-dependent manner. The bound protein was identified as calmodulin by dgel filtration of the sanjoinine-A bound cytosol fraction on a Sephadex G-75 column. Calmodulin was bound to sanjoinine-A bound at two sets of binding sites in the calculated as two at high affinity sites $(Kd=1.1\;mu{M)}$ and four at low affinity sites $(Kd=3.1\;\mu{M)}$ .

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  8. [국내논문]   Human renal dipeptidase from kidneys of renal stone patients: Partial purification  

    Park, Haeng Soon , Kim, Doh -Ha , Kwark, Hyun S. Ellen , Park, Sung Kwang , Kang, Sung Kyew , Chung, Byung Ho , Yoo, Gyrung Soo
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 295 - 299 , 1993 , 0253-6269 ,

    초록

    A binding protein of radio-labeled sanjoinine-A (fangufoline) in rat brain cytoplasm was investigated, using an equilibrium dialysis technique. The labeled agent was bound to the cytosol fraction with two distinctly different types of sets in calclum ion-dependent manner. The bound protein was identified as calmodulin by dgel filtration of the sanjoinine-A bound cytosol fraction on a Sephadex G-75 column. Calmodulin was bound to sanjoinine-A bound at two sets of binding sites in the calculated as two at high affinity sites $(Kd=1.1\;mu{M)}$ and four at low affinity sites $(Kd=3.1\;\mu{M)}$ .

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  9. [국내논문]   Genetic Transformation of Streptomyces caespitosus  

    Yoo, Jin-Cheol (Department of Pharmacy, Chosun University ) , Sim, Jung-Bo (Department of Pharmacy, Chosun University ) , Kim, Sung-Jin (Department of Genetic Engineering, Chosun University ) , Kim, Si-Wouk (Department of Environmental Science, KIST ) , Lee, Jung-Jun (Genetic Engineering Reserch Institute)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 300 - 304 , 1993 , 0253-6269 ,

    초록

    Genetic transformation of streptomyces gaespitosus by plasmid plJ 702 was camied out. Optimal conditions for the protoplast preparation of streptomyces casepitosus, its regeneration, and its transformation by plJ 702 were evaluated. Addition of 2% glycine to the culture broth was optimal for protoplast yield. Formation and regeneration of protoplasts were most efficient when the mycelium were harvested at between late log and stationary growth phase. The regeneration frequency of the protoplasts was 15% when the protoplats were regenerated on R2YE agar media containing 0.5M sucrose. Under the best condition for protoplats (M.W. 4,000) treatment for 2 minutes.

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  10. [국내논문]   Altered Cardiac $Na^+,K^+$-ATPase Activity in Prehypertensive Spontaneously Hypertensive Rat  

    Lee, Shin-Woong (College of Pharmacy, Yeungnam University ) , Lee, Jeung-Soo (College of Pharmacy, Yeungnam University ) , Wallick, Earl-T. (Department of Pharmacology and Cell Biophysics, University of Cincinnati, College of Medicine)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.16 no.4 ,pp. 305 - 311 , 1993 , 0253-6269 ,

    초록

    $Na^+,K^+$ -ATPase activity, $Na^+$ -dependent phosphorylation, and $[^3H]$ ouabain binding in sarcolemma prepared from 4 week old spontaneously hypertensive rat(SHR) ventricles were compared to the same parameters in sarcolemma from age matched nomotensive Wister-Kyoto (WKY) rat ventricles to examine whether the reduced myocardial $Na^+$ -pump activity in SHR is an inherited enzymatic defect or a second phenomenon due to sustained hypertension. The total body weights, ventricular weights, and blood pressures were the same for SHR and WKY. No significant differences in sarcolemmal protein content and protein recovery were noted between the two groups. Sarcolemma isolated from SHR ventricles showed significantly less $Na^+,K^+$ -ATPase activity ande number of phosphorylation sites when compared to sarcolemma from the WKY ventricles. Equilibrium binding of $[^3H]$ ouabain and the tumover number of myocardial $Na^+,K^+$ -ATPase, however, were the same for both groups. These reults indicate that the low affinity $(\alpha,\;or\;\alpha^1)\;\alpha$ isoform is the same in ventricles of SHR and WKY. The reduced amount of isoform of the $Na^+,K^+$ -ATPase inprehypetensive SHR ventricles may play some role in the development of hypertension.

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