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Applied soil ecology : a section of Agriculture, E... 49건

  1. [해외논문]   Editorial Board   SCI SCIE


    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. ii - ii , 2018 , 0929-1393 ,

    초록

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  2. [해외논문]   In vitro effect of artemisinin on microbial biomasses, enzyme activities and composition of bacterial community   SCI SCIE

    Liu, Hai (College of Resources and Environment, Southwest University, Chongqing 400716, China ) , Huang, Jianguo (College of Resources and Environment, Southwest University, Chongqing 400716, China ) , Yuan, Ling (College of Resources and Environment, Southwest University, Chongqing 400716, China)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 1 - 6 , 2018 , 0929-1393 ,

    초록

    Abstract The anti-malarial drug artemisinin is extracted from the leaves of Artemisia annua L. The potential risk of artemisinin released from leaf debris and root exudation of this medicinal plant is unknown for microbes in soils where commercial cultivation of A. annua L. is practiced. Thus, a soil incubation experiment was conducted to compare microbial biomass, enzyme activities, and bacterial community compositions by different concentrations of artemisinin (without artemisinin, CK; 10mgkg - 1 soil, LA; and 20mgkg - 1 soil, HA). The results showed that this compound decreased significantly soil microbial biomass carbon and nitrogen, and activities of dehydrogenases and urease, implying the inhibition on the growth, reproduction, and metabolism of some bacteria, taking into account of the facts that the microbial biomass includes those of bacteria and dehydrogenases cannot act on their own without viable bacterial hosts. There were 13064, 11738, and 10107 16S rDNA sequences picked up from soils of CK, LA, and HA, respectively, by MiSeq Illumina sequencing, which were attributed into 541 (CK), 453 (LA), 414 bacterial phylotypes (HA). Soils received artemisinin showed lower bacterial richness and diversity indexes than CK. Less group and number of bacteria present in soils could encourage over reproduction of any single bacterial taxon and thus increase the risk of plant disease occurrence. All soils studied were dominated by three bacterial phyla (Acidobacteria, Proteobacteria, and Actinobacteria), with these three phyla accounting for 60.32-70.43% of the total bacteria. However, most of bacterial phyla and predominant phylotypes varied significantly in their abundances between CK and artemisinin treatment. Among the top 20 predominant bacteria, five to seven were unique in each of soils studied. Therefore, artemisinin released from A. annua L. in cultivation might exhibit selective antibacterial action towards soil bacteria and cause great changes in bacterial activities and community structure, which could influence, at least in part, soil processes and functions. Highlights Artemisinin caused changes in bacterial community structure in soil. Artemisinin decreased significantly the activities of soil dehydrogenases and urease. A. annua L. cultivation might thus result in a risk for soil environment.

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  3. [해외논문]   Diversity of plant-parasitic nematode communities associated with olive nurseries in Morocco: Origin and environmental impacts   SCI SCIE

    Aï (Université) , t Hamza, Mohamed (Ibn Zohr, Faculté) , Moukhli, Abdelmajid (des Sciences d'Agadir, Laboratoire LBVRN, BP 8106, 80000 Agadir, Morocco ) , Ferji, Zahra (INRA, CRRA, BP 513, Menara, Marrakech, Morocco ) , Fossati-Gaschignard, Odile (Institut Agronomique et Vétérinaire Hassan II, Campus d'Agadir, Département de Protection des Plantes, BP 18/S, 80000 Agadir, Morocco ) , Tavoillot, Johannes (CBGP, IRD, CIRAD, INRA, Montpellier SupAgro, Univ. Montpellier, Montpellier, France ) , Ali, Nadine (CBGP, IRD, CIRAD, INRA, Montpellier SupAgro, Univ. Montpellier, Montpellier, France ) , Boubaker, Hassan (CBGP, IRD, CIRAD, INRA, Montpellier SupAgro, Univ. Montpellier, Montpellier, France ) , El Mousadik, Abdelhamid (Université) , Mateille, Thierry (Ibn Zohr, Faculté)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 7 - 16 , 2018 , 0929-1393 ,

    초록

    Abstract Plant-parasitic nematodes (PPN) are key impediments to efficient global crop production and impair the quality of susceptible plants in nurseries as well. In this context, nematode communities were determined in 305 solid substrate samples collected from 25 olive ( Olea. europaea. subsp. europaea ) nurseries in Morocco. Taxonomical and functional diversity as well as the structures of PPN communities were described and then compared between regions, cultivars as well as according to biotic and abiotic factors. A high diversity of PPN was observed, with the detection of 63 species and 26 genera. The most dominant taxa detected were spiral nematodes ( Helicotylenchus spp. and Rotylenchus spp.), stunt nematodes ( Tylenchorhynchus spp.), grazer nematodes ( Tylenchus spp.) and root-knot nematodes ( Meloidogyne spp.). Hoplolaimidae nematodes ( Helicotylenchus spp. and Rotylenchus spp.) and Tylenchus spp. were better adapted to rainy conditions that prevailed in the northern regions of Morocco. Multiblock analyses demonstrated that functional diversity ( cp and trophic groups) was more affected by the environment than taxonomical diversity (total number, species richness, locale diversity and evenness). They also indicated that PPN communities were more impacted by climatic variables (rainfall and minimum temperature) and nursery substrate origins than by soil physic-chemical factors. Nevertheless, a co-inertia analysis showed that N, P and K amendments in olive nurseries enhanced the development of harmful PPN, especially root-knot nematodes. Highlights Olive nurseries in Morocco are heavily infested with plant-parasitic nematodes (PPN). PPN are introduced by substrates originating from field, forest and river banks. Their spatial distribution is driven by the annual rainfall and the min. temperature. Their diversity is especially impacted by the field and forest origin of the substrates.

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  4. [해외논문]   Assessment of chemical, biochemical, and microbiological properties in an artisanal Zn-smelting waste slag site revegetated with four native woody plant species   SCI SCIE

    Luo, Youfa (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Wu, Yonggui (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Xing, Rongrong (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Wang, Hu (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Shu, Jie (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Wu, Zhixue (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China ) , Wan, Zurong (College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, Guizhou, China)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 17 - 26 , 2018 , 0929-1393 ,

    초록

    Abstract Waste slag from artisanal Zn smelting causes serious environmental problems in China due to the dispersion of toxic metals in nearby water or agricultural ecosystems. Revegetation with native plant species stabilizes the surface of waste slag and reduces the potential risk to the ecosystem. A 4-year field investigation was conducted to examine the effect of revegetating a Zn-smelting site with four woody plant species, i.e., Robinia pseudoacacia L., Broussonetia papyrifera L., Cryptomeria fortunei L., and Arundo donax L., on the physicochemical, biochemical, and microbiological properties of the waste slag. Revegetation significantly increased the nutrient (N, P, and K) and organic matter contents, enzyme activities (invertase, neutral phosphatase, urease, and catalase), microbial biomass, and three dominant microbial (bacteria, fungi, and actinomycetes) community populations. Revegetation reduced Pb (except for R. pseudoacacia ) and increased Cd (except for B. papyrifera ) and Zn bioavailability in the rhizosphere of the plant species compared with the bare slag. In addition, there was an increase (in the presence of B. papyrifera and C. fortunei ) or decrease (in the presence of R. pseudoacacia and A. donax ) in Cu bioavailability in the plant rhizosphere. The nutrient contents were significantly positively correlated with microbial biomass and enzyme activity; however, the chemical properties (pH, EC, and available heavy metals) were poorly correlated with the biochemical and microbiological properties of the waste slag. The waste slag microenvironment was significantly improved by the four woody plant species. Furthermore, B. papyrifera exhibited the best performance with respect to enhancing the development of the micro-environmental conditions of the slag and is suitable as a native woody plant species for revegetating Zn-smelting waste slag sites. Highlights Difference in ecological restoration potential was estimated in four native woody plants. Revegetation significantly enhanced development of the microenvironment of Zn-smelting slag. Biochemical parameters are good indicators of changes in Zn-smelting slag. B. papyrifera exhibited greater potential for revegetation of Zn-smelting slag.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  5. [해외논문]   Effects of metam sodium fumigation on the abundance, activity, and diversity of soil bacterial communities   SCI SCIE

    Sederholm, Maya R. (Water & Energy Sustainable Technology (WEST) Center, The University of Arizona, 2959 W. Calle Agua Nueva, Tucson, AZ 85745, USA ) , Schmitz, Bradley W. (Department of Civil & Environmental Engineering, National University of Singapore, Block EIA, #07-03, No. 1 Engineering Drive 2, 117576, Singapore ) , Barberá (Department of Soil, Water and Environmental Science, The University of Arizona, 1177 E. 4th Street, Tucson, AZ 85721, USA ) , n, Albert (Water & Energy Sustainable Technology (WEST) Center, The University of Arizona, 2959 W. Calle Agua Nueva, Tucson, AZ 85745, USA) , Pepper, Ian L.
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 27 - 33 , 2018 , 0929-1393 ,

    초록

    Abstract Metam sodium is a fumigant used as a crop pretreatment in agriculture to control a wide array of pests, and soil borne diseases that may adversely affect plant quantity and quality. This present study utilized control and treated field plots to examine the effects of metam sodium on indigenous soil microbes in terms of numbers, activity, and diversity. Following metam sodium application, culturable heterotrophic counts in all soils increased significantly for 24h, but quickly returned back to original levels. Application resulted in decreased microbial activity, detected by ATP-based assays, that was significantly lower in treated soils than control plots, but recovered quickly. High-throughput sequencing of the 16S rRNA gene showed that treated plots contained significantly lower numbers of observed OTUs, particularly 14, 21, and 28days after treatment. Soil bacterial communities were significantly altered by MS-treatment due to increased relative abundances of Actinomycetales, Bacilli, and Chloroflexi, as well as decreased Acidobacteria. These dominant taxa observed in MS-treated plots are major contributors to biological activity in various healthy soils and rhizospheres. Therefore, the increase in relative abundance of these biologically productive phyla coupled with abundant ATP production suggests that soil health recovered following MS-treatment and remained functionally intact. Highlights Metam sodium is utilized as a soil fumigant, but has detrimental biocidal effects. Metam sodium adversely affects soil microbial bacterial communities and decreases activity. Fumigation results in a divergence of soil bacterial community composition. Productive phyla recover rapidly in terms of numbers, activity, and diversity. Following MS treatment, soil health recovered and was functionally intact.

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  6. [해외논문]   Engineered production of 2,4-diacetylphloroglucinol in the diazotrophic endophytic bacterium Pseudomonas sp. WS5 and its beneficial effect in multiple plant-pathogen systems   SCI SCIE

    Patel, Janki K. (Corresponding author.) , Archana, G.
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 34 - 44 , 2018 , 0929-1393 ,

    초록

    Abstract The 2,4-diacetylphloroglucinol (2,4-DAPG) biosynthetic gene clusters were amplified from Pseudomonas protegens Pf-5 and Pseudomonas sp. G22 (lab isolate) and cloned into pUCPM18Gm vector for constitutive expression in diazotrophic endophytic Pseudomonas sp. WS5 isolated from wheat plant. 2,4-DAPG production in recombinant strains as quantitated by HPLC analysis was 12–14μgml −1 while the native 2,4-DAPG producing strains showed −1 . The antifungal extract of the recombinant strains showed antagonistic effect against Magnaporthe oryzae B157 and Rhizoctonia solani . Endophytic colonization and plant protection ability of recombinant strains was observed in rice, sorghum, and wheat after 60 d of growth in plants challenged with the fungal pathogens, indicating the endophytic recombinant strain was effective in promoting the plant growth during infection. Pathogenesis-related genes expression in rice plants as analyzed by qPCR showed that the rice plants colonized by 2,4-DAPG producing recombinant strain when challenged with M. oryzae B157 had elevated levels of NPR1 and PR10a gene expression. Highlights Endophytic bacterial system used for expression of 2, 4- DAPG gene cluster. High 2, 4- DAPG production was found in genetically modified Pseudomonas sp. WS5. The 2, 4-DAPG extract found to inhibit the growth of two important cereal fungal pathogens. Resistance development in plants upon the Pseudomonas sp. WS5 colonization. Genetically modified strains able to sustain the plant growth during pathogen attack.

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  7. [해외논문]   The root endophytic fungus Trichoderma atroviride induces foliar herbivory resistance in maize plants   SCI SCIE

    Contreras-Cornejo, Hexon Angel (Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autónoma de México, Antigua Carretera a Pátzcuaro No. 8701, Ex-Hacienda de San José) , Mací (de La Huerta, C.P. 58190, Morelia, Michoacán, Mexico ) , as-Rodrí (Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, C.P. 58030, Morelia, Michoacán, Mexico ) , guez, Lourdes (Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autónoma de México, Antigua Carretera a Pátzcuaro No. 8701, Ex-Hacienda de San José) , del-Val, Ek (de La Huerta, C.P. 58190, Morelia, Michoacán, Mexico ) , Larsen, John (Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autónoma de México, Antigua Carretera a Pátzcuaro No. 8701, Ex-Hacienda de San José)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 45 - 53 , 2018 , 0929-1393 ,

    초록

    Abstract Plant roots naturally associate with Trichoderma spp., which can promote plant health and nutrition. Despite that Trichoderma spp. are well-known biocontrol agents, information on their effects against foliar insect herbivory is limited. Here, we examined the effects of T. atroviride in providing maize ( Zea mays ) resistance against the insect herbivore Spodoptera frugiperda . Increased plant growth, reduced herbivory and altered insect feeding pattern were observed after maize inoculation with T. atroviride . Plant protection was correlated with increased emission of volatile terpenes and accumulation of jasmonic acid, an activator of defense responses against herbivory. Chemical analyses revealed that T. atroviride produced the volatiles 1-octen-3-ol and 6-pentyl-2 H -pyran-2-one. Pharmacological tests showed that both compounds reduced the consumption of foliar tissue and altered the feeding pattern of S. frugiperda in a similar way to T. atroviride . These results provide new insight into the role of T. atroviride in plant health in terms of induction of resistance to insect herbivory and production of antifeedant secondary metabolites. Highlights T. atroviride induces foliar hebivore resistance in maize plants. Fungal root colonization triggers plant defense responses against the herbivore insect S. frugiperda . Fungus produces both 1-octen-3-ol and 6-PP, which in turn restrict plant damage by S. frugiperda . 1-octen-3-ol and 6-PP alter the feeding partner of S. frugiperda . Graphical abstract [DISPLAY OMISSION]

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  8. [해외논문]   Long and short term effects of solarization on soil microbiome and agricultural production   SCI SCIE

    Kanaan, Housam (Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel ) , Frenk, Sammy (Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel ) , Raviv, Michael (Institute of Plant Sciences, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel ) , Medina, Shlomit (Institute of Plant Sciences, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel ) , Minz, Dror (Institute for Soil, Water and Environmental Sciences ARO, Volcani Research Center, 68 HaMacabim Rd. P.O Box 15159, Rishon Lezion 7528809, Israel)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 54 - 61 , 2018 , 0929-1393 ,

    초록

    Abstract Soil solarization, a method of chemical-free pest treatment, is a practical and cost-effective way to treat organic farming soil. This method uses polyethylene sheets to capture solar irradiation that heats the soil. Together with heat generated during composting of fresh organic matter, this method can effectively treat many soil-borne pathogens. In this study we examined the effects of solarization with the addition of compost at three maturation levels, on soil microbial community structure and function along with plant performance. Similar hydrolytic activity among the treatments (as measured by FDA hydrolysis) was measured in the first year of solarization. High level of activity was obtained in soil amended with immature compost at the beginning of the second year, suggesting residual (carry-over) effects of previous-year treatments. Amplicon sequencing of 16S rRNA encoding genes was used to study soil bacterial community structure. Diversity and richness of bacterial communities were found to be negatively affected by solarization in all treatments. Interestingly, bacterial communities of solarized soils clustered together, regardless of compost amended and type, separated from the cluster of communities from non-solarized soils of all amendments. Specifically, long and short terms negative effects of solarization on the relative abundance of Alphaproteobacteria, Acidobacteria and Actinobacteria were noticed. In contrast, solarization positively affected Bacilli and Gammaproteobacteria abundance in both long and short term. Furthermore, solarization had long and short term positive effects on productivity of eggplant and wheat plants. This study is the first to describe in high details the combined effects of solarization and amendment of composts of different maturation levels. Highlights Solarization resulted in significant shifts in bacterial community composition: increased relative abundance of Bacilli and Gammaproteobacteria and decreased abundance of Actinobacteria, Alphaproteobacteria and Acidobacteria. Solarization reduced Shannon diversity and richness indices while increased crop productivity. Compost amendments had a significant effect on bacterial community composition, increasing diversity and richness at time 0 only. Amendment of all types of compost had no effect on plant biomass of the two test plants.

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  9. [해외논문]   Effect of black alder (Alnus glutinosa) admixture to Scots pine (Pinus sylvestris) plantations on chemical and microbial properties of sandy mine soils   SCI SCIE

    Sroka, Katarzyna (AGH University of Science and Technology, Department of Environmental Management and Protection, al. Mickiewicza 30, 30-059 Kraków, Poland ) , Chodak, Marcin (AGH University of Science and Technology, Department of Environmental Management and Protection, al. Mickiewicza 30, 30-059 Kraków, Poland ) , Klimek, Beata (Jagiellonian University, Institute of Environmental Sciences, Faculty of Biology and Earth Sciences, ul. Gronostajowa 7, 30-387 Kraków, Poland ) , Pietrzykowski, Marcin (University of Agriculture in Kraków, Department of Forest Ecology and Reclamation, Institute of Ecology and Silviculture, Faculty of Forestry, al. 29 Listopada, 31-425 Kraków, Poland)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 62 - 68 , 2018 , 0929-1393 ,

    초록

    Abstract Phytomelioration and tree species selection on poor mine soils are important for reclamation and afforestation success. Black alder ( Alnus glutinosa ), as N-fixing species, is often planted on afforested post-mining barrens as an admixture to improve soil properties and enhance growth of target tree species. Objective of this study was to assess the effect of black alder admixture on chemical, physical and microbial properties of sandy mine soils afforested with Scots pine ( Pinus sylvestris ). The topsoil samples (0–5cm) were taken in areas afforested with Scots pine with black alder admixture at 1m, 2m, 4m and 8m from the black alder rows and measured for texture, maximum water holding capacity (WHC), pH, C org and N contents. The measured soil microbial properties included microbial biomass (C mic ), basal respiration rate (RESP), N mineralization rate and urease activity (URE). Community level physiological profiles (CLPP) of soil microbial communities were determined with Biolog ? Ecoplates. The highest C org , N, C mic , RESP, N mineralization rate and URE values were measured at 1m from the alders row and then decreased rapidly. The influence of alders ranged to ca 2m–3m from the alders row depending on the soil property. Soil microbial communities close to the alders row were functionally more diverse than those in soils from larger distances. There were distinct differences in CLPPs between microbial communities from soil at 1m and 8m from the alders row wherein those close to the alders were more efficient in degradation of carbohydrates and those at 8m in degradation of polymers. Results of the study clearly indicate positive influence of black alder on several soil properties crucial for their biological activity and fertility. Highlights Black alder increases C org and N content in sandy mine soils. Black alder improves microbial properties of sandy mine soils. Significant differences in soil microbial CLPPs under black alder and Scots pine. Effect of alder evident up to 2–3m from the alders row.

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  10. [해외논문]   Distribution and utilization of nitrogen on moderately and heavily grazed temperate desert steppe using the 15N tracing technique   SCI SCIE

    Jiao, Ting (College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou, People's Republic of China ) , Wu, Jianping (College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, People's Republic of China ) , Zhao, Shengguo (College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, People's Republic of China ) , Li, Yajuan (College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou, People's Republic of China ) , Zhao, Guiqin (College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou, People's Republic of China ) , Lei, Zhaomin (College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, People's Republic of China)
    Applied soil ecology : a section of Agriculture, Ecosystems & Environment v.124 ,pp. 69 - 74 , 2018 , 0929-1393 ,

    초록

    Abstract Due to overgrazing, grasslands in northern China have been severely degraded since 1980s. Grassland biomass and plant populations decreased dramatically. Application of fertilizer is a common technique for pasture management in many countries, however, it has not been widely used on China grasslands. Nitrogen (N) is an important productivity driver in grassland ecosystems, but its transportation, allocation and utilization in temperate desert steppe remained unclear. In this study, a sequential nitrogen addition experiment was conducted on a farm in southwestern Jingtai County, Gansu Province, in the transition zone between Loess Plateau and Tengger Desert. Two different grassland plots with moderate grazing (MG) and heavy grazing (HG) were fenced in 2008. N (NH 4 NO 3 ) was applied at the rate of: 12.25gN·m −2 , 24.5gN·m −2 , 36.75gN·m −2 and 49gN·m −2 , respectively. To determine the amount and distribution of nitrogen applied to the grassland, the 15 N tracer technique was adopted. The results demonstrated that the recovery rates of 15 N ranged from 3.8% to 11.7% in the plants. The nitrogen recovery amount from both shoots and roots increased significantly ( P 0.05), while the recovery rate decreased gradually with increasing nitrogen application. The 15 N recovery from shoots under HG was much less than from MG. In one growing season, only 18.2%–8.0% of labeled 15 N was derived from fertilizer, so soil nitrogen is still the main source absorbed and utilized by plants. As much as 50.4%–84.4% labeled 15 N remained in soil, which was evenly distributed throughout 0–20cm soil layer, and significantly increased with nitrogen application rates. The nitrogen remained in the top soil (0–10cm) pool was much less than that of 10–20cm layer ( P 0.05). The nitrogen loss amounts and rates were significantly different among N treatments, which indicated that there was an increasing trend in N loss with increasing N application rates. Loss/recovery analysis showed that 12.25gN·m −2 was the recommended application rate. This work is expected to provide the lowest risk/return ratio and produce both economic and environmental benefits for the degraded grassland in Gansu and analogus regions in China. Highlights Grazing intensity negatively affected the nitrogen (N) recovery by plants. Soil N was still the main N source absorbed and utilized by plants. With increasing N application, the N loss increased in temperate desert steppe. 12.25gN·m −2 was the recommended for the degraded grassland in Gansu.

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